Detection of Human FHL1 by Western Blot.
Western blot shows lysates of human skeletal muscle tissue and human heart tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human FHL1 Monoclonal Antibody (Catalog # MAB5938) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FHL1 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
FHL1 in SH‑SY5Y Human Cell Line.
FHL1 was detected in immersion fixed SH‑SY5Y human neuroblastoma cell line using Mouse Anti-Human FHL1 Monoclonal Antibody (Catalog # MAB5938) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
FHL1 (Four-and-a-half LIM domains protein 1; also SLIM1 and KyoT1) is an approximately 35 kDa member of the four-and-a half class of the LIM domain-only protein family. It is highly expressed in myocardium and skeletal muscle, and is now known to bind NFATc1, promoting muscle hypertrophy. It is also noted to be a RIP140 binding partner that inhibits estrogen receptor signaling. Human FHL1 is 323 amino acids (aa) in length. It contains one C4-type Zn-finger region (aa 7-31), three distinct LIM domains (aa 40-77; 101-158; 162-212) and a bipartite NLS (aa 231-246). There is one 32 kDa splice variant that shows a 50 aa substitution for aa 231-323. This creates a fourth LIM domain. There are also alternative start sites 29 aa and 16 aa upstream of the standard site, a deletion of aa 168-296, and an 11 aa substitution for aa 81-91. Over aa 93-166, human FHL1 is 97% aa identical to mouse FHL1.
Four and a Half LIM Domains Protein 1
Entrez Gene IDs:
2273 (Human); 14199 (Mouse); 25177 (Rat)
bA535K18.1; FHL1; four and a half LIM domains 1; HLH1; HPLH1; KYOT; KYO-T; LIM protein SLIMMER; skeletal muscle LIM-protein1; SLIM1; SLIM1Isl-1 and Mec-3 domains 1; SLIMMER; XMPMA
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