Human GATA-4 Antibody

Catalog # Availability Size / Price Qty
MAB2606
MAB2606-SP
Detection of Human GATA‑4 by Western Blot.
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Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (2)

Human GATA-4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human GATA-4 in Western blots.
Source
Monoclonal Mouse IgG2B Clone # 532020
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human GATA-4
Met27-Phe211
Accession # P43694
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

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Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human GATA‑4 antibody by Western Blot. View Larger

Detection of Human GATA‑4 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and human liver tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human GATA-4 Monoclonal Antibody (Catalog # MAB2606) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GATA-4 at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GATA-4

GATA-4 belongs to the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. It is widely expressed in mesodermal- and endodermal-derived tissues. GATA-4 interacts with other transcription factors such as NKX2.5, and myocyte enhancer factor 2 to regulate cardiac myocyte-specific gene expression. GATA-4 contains 2 zinc finger-binding domains and migrates at 56 kDa in SDS-PAGE gels. Human GATA-4 share 93%, 93% and 92% amino acid sequence identity with porcine, rat and mouse GATA-4, respectively.

Entrez Gene IDs
2626 (Human)
Alternate Names
GATA binding protein 4; GATA4; GATA-4; GATA-binding factor 4; GATA-binding protein 4; MGC126629; transcription factor GATA-4

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Citations for Human GATA-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. GATA4 induces liver fibrosis regression by deactivating hepatic stellate cells
    Authors: N Arroyo, L Villamayor, I Díaz, R Carmona, M Ramos-Rodr, R Muñoz-Chap, L Pasquali, MG Toscano, F Martin, DA Cano, A Rojas
    JCI Insight, 2021;0(0):.
    Species: Mouse
    Sample Types: Tissue Lysates
    Applications: Western Blot
  2. Electromechanical Conditioning of Adult Progenitor Cells Improves Recovery of Cardiac Function After Myocardial Infarction
    Authors: A Llucià-Val, C Soler-Boti, C Gálvez-Mon, S Roura, C Prat-Vidal, I Perea-Gil, B Sanchez, R Bragos, G Vunjak-Nov, A Bayes-Geni
    Stem Cells Transl Med, 2017;6(3):970-981.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human GATA-4 Antibody

Average Rating: 5 (Based on 2 Reviews)

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Human GATA-4 Antibody
By Anonymous on 12/10/2018
Application: IHC Sample Tested: Adult pancreas Species: Mouse

Paraffin-embedded sections
Antigen retrieval: Citrate pH=6
Mouse pancreas


Human GATA-4 Antibody
By Anonymous on 03/31/2018
Application: WB Sample Tested: Mesenchymal stem cells Species: Human