Human GRK3 Antibody

Catalog # Availability Size / Price Qty
MAB47851-100
MAB47851-SP
Detection of Human GRK3 by Western Blot.
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Human GRK3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human GRK3 in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 497007
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human GRK3
Pro469-Leu688
Accession # P35626
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-25 µg/mL
See below
Immunocytochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human GRK3 antibody by Western Blot. View Larger

Detection of Human GRK3 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and CEM human T-lymphoblastoid cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human GRK3 Monoclonal Antibody (Catalog # MAB47851) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for GRK3 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry GRK3 antibody in CEM-NKr Human Cell Line by Immunocytochemistry (ICC). View Larger

GRK3 in CEM-NKr Human Cell Line. GRK3 was detected in immersion fixed CEM-NKr human Natural Killer cell resistant T-lymphoblastoid cell line using Mouse Anti-Human GRK3 Monoclonal Antibody (Catalog # MAB47851) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry GRK3 antibody in Human Prostate Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

GRK3 in Human Prostate Cancer Tissue. GRK3 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-Human GRK3 Monoclonal Antibody (Catalog # MAB47851) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GRK3

G protein-coupled receptor kinases (GRKs) are important modulators of G protein-coupled receptor (GPCR) signaling. Receptor phosphorylation by specific GRKs plays a key role in triggering rapid desensitization. The GRK family consists of 7 isoforms that share a central catalytic domain with homology to other serine/threonine kinases. The catalytic domain is flanked by an amino-terminal RGS domain of 183‑188 amino acids and a carboxyl-terminus of variable length. GRK3 (also known as beta -adrenergic receptor kinase 2, or beta ARK2) and GRK2 ( beta ARK1) are members of the beta -adrenergic receptor kinase subfamily. GRK3 may phosphorylate alpha -synuclein at S129, suggesting a potential involvement for this kinase in Parkinson’s disease.

Long Name
G protein-coupled Receptor Kinase 3
Entrez Gene IDs
157 (Human); 320129 (Mouse); 25372 (Rat)
Alternate Names
ADRBK2; adrenergic, beta, receptor kinase 2; BARK2; BARK2beta-adrenergic receptor kinase 2; Beta-ARK-2; EC 2.7.11; EC 2.7.11.15; G-protein-coupled receptor kinase 3; GRK3; GRK3Beta-ARK-2

Product Datasheets

FAQs

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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