Human GRK3 Antibody Summary
Accession # P35626
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human GRK3 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and CEM human T-lymphoblastoid cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human GRK3 Monoclonal Antibody (Catalog # MAB47851) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for GRK3 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
GRK3 in CEM-NKr Human Cell Line. GRK3 was detected in immersion fixed CEM-NKr human Natural Killer cell resistant T-lymphoblastoid cell line using Mouse Anti-Human GRK3 Monoclonal Antibody (Catalog # MAB47851) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
GRK3 in Human Prostate Cancer Tissue. GRK3 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-Human GRK3 Monoclonal Antibody (Catalog # MAB47851) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
G protein-coupled receptor kinases (GRKs) are important modulators of G protein-coupled receptor (GPCR) signaling. Receptor phosphorylation by specific GRKs plays a key role in triggering rapid desensitization. The GRK family consists of 7 isoforms that share a central catalytic domain with homology to other serine/threonine kinases. The catalytic domain is flanked by an amino-terminal RGS domain of 183‑188 amino acids and a carboxyl-terminus of variable length. GRK3 (also known as beta -adrenergic receptor kinase 2, or beta ARK2) and GRK2 ( beta ARK1) are members of the beta -adrenergic receptor kinase subfamily. GRK3 may phosphorylate alpha -synuclein at S129, suggesting a potential involvement for this kinase in Parkinson’s disease.
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human GRK3 Antibody
There are currently no reviews for this product. Be the first to review Human GRK3 Antibody and earn rewards!
Have you used Human GRK3 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image