Human HNF-4 alpha/NR2A1 Antibody (843716)

R&D Systems | Catalog # MAB4605

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Intracellular Staining by Flow Cytometry, Immunocytochemistry, Simple Western

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 843716
View all HNF-4 alpha/NR2A1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human HNF‑4 alpha /NR2A1
Val130-Ser330
Accession # P41235

Specificity

Detects human HNF‑4 alpha /NR2A1 in ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human HNF-4 alpha/NR2A1 Antibody (843716)

Detection of Human HNF-4a/NR2A1 antibody by Western Blot.

Detection of Human HNF‑4 alpha /NR2A1 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. Gels were loaded with 10 µg of cytoplasmic (Cyto) and 5 µg of nuclear (Nuc) extracts. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human HNF-4a/ NR2A1 Monoclonal Antibody (Catalog # MAB4605) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for HNF-4a/NR2A1 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
HNF-4a/NR2A1 antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC).

HNF-4 alpha /NR2A1 in HepG2 Human Cell Line.

HNF-4a/NR2A1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human HNF-4a/NR2A1 Monoclonal Antibody (Catalog # MAB4605) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human HNF-4a/NR2A1 antibody by Simple WesternTM.

Detection of Human HNF‑4 alpha /NR2A1 by Simple WesternTM.

Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for HNF‑4 alpha /NR2A1 at approximately 62 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human HNF‑4 alpha /NR2A1 Monoclonal Antibody (Catalog # MAB4605). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Detection of HNF-4 alpha/NR2A1 antibody in HepG2 Human Cell Line antibody by Flow Cytometry.

Detection of HNF-4 alpha/NR2A1 in HepG2 Human Cell Line by Flow Cytometry.

HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human HNF-4 alpha/NR2A1 Monoclonal Antibody (Catalog # MAB4605, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Detection of HNF-4 alpha/NR2A1 antibody in Human Endodermal Cells antibody by Flow Cytometry.

Detection of HNF-4 alpha/NR2A1 in Human Endodermal Cells by Flow Cytometry.

BG01V human embryonic stem cell line differentiated to endodermal cells (StemXVivo Endoderm Kit, Catalog # SC019B) was stained with Mouse Anti-Human HNF-4 alpha/NR2A1 Monoclonal Antibody (Catalog # MAB4605, filled histogram or isotype control antibody (Catalog # MAB0041, open histogram) followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Applications for Human HNF-4 alpha/NR2A1 Antibody (843716)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: BG01V human embryonic stem cell line differentiated to endodermal cells (StemXVivo Endoderm Kit, Catalog # SC019B) and HepG2 human hepatocellular carcinoma cell line fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Permeabilization Buffer Kit (Catalog # FC012).

Simple Western

10 µg/mL
Sample: Human liver tissue

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line

Reviewed Applications

Read 2 reviews rated 5 using MAB4605 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HNF-4 alpha/NR2A1

HNF-4 alpha is a transcription factor that binds DNA as a homodimer. HNF‑4 alpha is important in liver, kidney, and intestinal development. It has also been intensely studied as one of a variety of genes responsible for diabetes mellitus. HNF-4 alpha has been shown in knock out mice to be essential for the morphogenic and functional differentiation of hepatocytes. HNF-4 alpha is a dominant regulator of epithelial phenotypes able to drive the mesenchymal-to-epithelial transition when expressed in fibroblasts.

Long Name

Hepatocyte Nuclear Factor-4, alpha

Alternate Names

HNF4 alpha, HNF4A, MODY1, NR2A1, TCF14

Entrez Gene IDs

3172 (Human)

Gene Symbol

HNF4A

UniProt

P41235

Additional HNF-4 alpha/NR2A1 Products

Product Documents for Human HNF-4 alpha/NR2A1 Antibody (843716)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human HNF-4 alpha/NR2A1 Antibody (843716)

For research use only

Citations for Human HNF-4 alpha/NR2A1 Antibody (843716)

Customer Reviews for Human HNF-4 alpha/NR2A1 Antibody (843716) (2)

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Customer Images

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  • Human HNF-4 alpha /NR2A1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: HepG2 Human Cell Line
    Species: Human
    Verified Customer | Posted 02/10/2022
    Human HNF-4 alpha/NR2A1 Antibody (843716) MAB4605
  • Human HNF-4 alpha /NR2A1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Pig trabecular meshwork cells
    Species: Pig
    Verified Customer | Posted 12/05/2016
    Detection of HNF-4a in cultured pig trabecular meshwork cells using Human HNF-4a/NR2A1 Antibody (MAB4605) at 1:500 dilution overnight and donkey anti-mouse Alexa 488 (1:1000) for 1 hour.
    Human HNF-4 alpha/NR2A1 Antibody (843716) MAB4605

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Protocols

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FAQs

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Associated Pathways

TGF-beta Signaling Pathways
TGF-beta Signaling Pathway Thumbnail