Human HNF-4 alpha /NR2A1 Antibody

Catalog # Availability Size / Price Qty
MAB4605
MAB4605-SP
Detection of Human HNF‑4 alpha /NR2A1 by Western Blot.
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Product Details
Citations (1)
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Reviews (1)

Human HNF-4 alpha /NR2A1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human HNF‑4 alpha /NR2A1 in ELISAs and Western blots.
Source
Monoclonal Mouse IgG2B Clone # 843716
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human HNF‑4 alpha /NR2A1
Val130-Ser330
Accession # P41235
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human HNF-4a/NR2A1 antibody by Western Blot. View Larger

Detection of Human HNF‑4 alpha /NR2A1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. Gels were loaded with 10 µg of cytoplasmic (Cyto) and 5 µg of nuclear (Nuc) extracts. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human HNF-4a/ NR2A1 Monoclonal Antibody (Catalog # MAB4605) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for HNF-4a/NR2A1 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry HNF-4a/NR2A1 antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC). View Larger

HNF-4 alpha /NR2A1 in HepG2 Human Cell Line. HNF-4a/NR2A1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human HNF-4a/NR2A1 Monoclonal Antibody (Catalog # MAB4605) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western Detection of Human HNF-4a/NR2A1 antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human HNF‑4 alpha /NR2A1 by Simple WesternTM. Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for HNF‑4 alpha /NR2A1 at approximately 62 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human HNF‑4 alpha /NR2A1 Monoclonal Antibody (Catalog # MAB4605). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Intracellular Staining by Flow Cytometry Detection of HNF-4 alpha/NR2A1 antibody in HepG2 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of HNF-4 alpha/NR2A1 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human HNF-4 alpha/NR2A1 Monoclonal Antibody (Catalog # MAB4605, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Intracellular Staining by Flow Cytometry Detection of HNF-4 alpha/NR2A1 antibody in Human Endodermal Cells antibody by Flow Cytometry. View Larger

Detection of HNF-4 alpha/NR2A1 in Human Endodermal Cells by Flow Cytometry. BG01V human embryonic stem cell line differentiated to endodermal cells (StemXVivo Endoderm Kit, Catalog # SC019B) was stained with Mouse Anti-Human HNF-4 alpha/NR2A1 Monoclonal Antibody (Catalog # MAB4605, filled histogram or isotype control antibody (Catalog # MAB0041, open histogram) followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HNF-4 alpha/NR2A1

HNF-4 alpha is a transcription factor that binds DNA as a homodimer. HNF‑4 alpha is important in liver, kidney, and intestinal development. It has also been intensely studied as one of a variety of genes responsible for diabetes mellitus. HNF-4 alpha has been shown in knock out mice to be essential for the morphogenic and functional differentiation of hepatocytes. HNF-4 alpha is a dominant regulator of epithelial phenotypes able to drive the mesenchymal-to-epithelial transition when expressed in fibroblasts.

Long Name
Hepatocyte Nuclear Factor-4, alpha
Entrez Gene IDs
3172 (Human)
Alternate Names
FLJ39654; hepatic nuclear factor 4 alpha; hepatocyte nuclear factor 4, alpha; hepatocyte nuclear factor 4-alpha; HNF4 alpha; HNF-4 alpha; HNF4A; HNF4a7; HNF-4-alpha; HNF4alpha10/11/12; HNF4HNF4a8; MODY; MODY1; NR2A1; NR2A1HNF4a9; NR2A21; Nuclear receptor subfamily 2 group A member 1; TCF; TCF14; TCF-14; TCF14HNF4alpha; Transcription factor 14; Transcription factor HNF-4; transcription factor-14

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Citation for Human HNF-4 alpha /NR2A1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Synergy of 5-aminolevulinate supplement and CX3CR1 suppression promotes liver regeneration via elevated IGF-1 signaling
    Authors: Chen, L;Zhang, L;Jin, G;Liu, Y;Guo, N;Sun, H;Jiang, Y;Zhang, X;He, G;Lv, G;Yang, J;Tu, X;Dong, T;Liu, H;An, J;Si, G;Kang, Z;Li, H;Yi, S;Chen, G;Liu, W;Yang, Y;Ou, J;
    Cell reports
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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Human HNF-4 alpha /NR2A1 Antibody
By Anonymous on 02/10/2022
Application: Immunocytochemistry/Immunofluorescence Sample Tested: HepG2 Human Cell Line Species: Human