Human Hormone-sensitive Lipase/HSL Antibody

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Detection of Human and Mouse Hormone-sensitive Lipase/HSL by Western Blot.
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Human Hormone-sensitive Lipase/HSL Antibody Summary

Species Reactivity
Detects human Hormone-sensitive Lipase/HSL in direct ELISAs.
Monoclonal Mouse IgG2B Clone # 729923
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Hormone-sensitive Lipase/HSL
Accession # Q05469
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse Hormone-sensitive Lipase/HSL antibody by Western Blot. View Larger

Detection of Human and Mouse Hormone-sensitive Lipase/HSL by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, NTera-2 human testicular embryonic carcinoma cell line, SVEC4-10 mouse vascular endothelial cell line, and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Hormone-sensitive Lipase/HSL Monoclonal Antibody (Catalog # MAB7104) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for Hormone-sensitive Lipase/HSL at approximately 70 kDa and 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Hormone-sensitive Lipase/HSL antibody in Human Testis by Immunohistochemistry (IHC-P). View Larger

Hormone-sensitive Lipase/HSL in Human Testis. Hormone-sensitive Lipase/HSL was detected in immersion fixed paraffin-embedded sections of human testis using Mouse Anti-Human Hormone-sensitive Lipase/HSL Monoclonal Antibody (Catalog # MAB7104) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of spermatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

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Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Hormone-sensitive Lipase/HSL

HSL (hormone-sensitive lipase), also called LIPE, is an 84 kDa phosphoprotein that is the rate-limiting enzyme in the catalytic breakdown of triglycerides. The human 775 amino acid (aa) form is active in adipose tissue and skeletal muscle. Within the region used as an immunogen, human HSL shares 92% and 93% aa sequence identity with mouse and rat HSL, respectively. HSL translocates to triglyceride-metabolizing lipid droplets in response to epinephrine or contraction in skeletal muscle, but to the cytosol following insulin treatment of adipocytes. A 120 kDa isoform of human HSL with a 301 aa N-terminal extension is present in the testes, localized to elongating spermatids and spermatozoa.

Entrez Gene IDs
3991 (Human); 16890 (Mouse); 25330 (Rat)
Alternate Names
hormone-sensitive lipase testicular isoform; Hormonesensitive Lipase; Hormone-sensitive Lipase; HSL; HSLEC; LHS; lipase, hormone-sensitive; LIPE

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