Human ICAT Antibody

Catalog # Availability Size / Price Qty
MAB3016-100
MAB3016-SP

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ICAT in Human Fibrocystic Breast Tissue.
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Human ICAT Antibody Summary

Species Reactivity
Human
Specificity
Detects human ICAT in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 536017
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human ICAT
Met1-Gln81
Accession # Q9NSA3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry ICAT antibody in Human Fibrocystic Breast Tissue by Immunohistochemistry (IHC-P). View Larger

ICAT in Human Fibrocystic Breast Tissue. ICAT was detected in immersion fixed paraffin-embedded sections of human fibrocystic breast tissue using Mouse Anti-Human ICAT Monoclonal Antibody (Catalog # MAB3016) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAT

Human ICAT is a physiological inhibitor of the beta -Catenin/Wnt signaling pathway. It is 9 kDa in size and found in both cytosol and nucleus. The molecule contains an alpha ‑helical N-terminal region (aa 9‑54) and an acidic, extended C-terminal region (aa 55‑78). The alpha -helical region binds to beta -Catenin, displacing CBP/p300, while the acidic C-terminus interacts with the beta -Catenin N-terminus, blocking Tcf interaction. Human ICAT shares 97% aa sequence identity with mouse and bovine ICAT, respectively.

Long Name
Beta-Catenin-Interacting Protein
Entrez Gene IDs
56998 (Human)
Alternate Names
beta-catenin-interacting protein ICAT; catenin, beta interacting protein 1; CTNNBIP1; ICAT; ICATbeta-interacting protein 1; Inhibitor of beta-catenin and Tcf-4

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Citation for Human ICAT Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. miR-26b-5p/TCF-4 Controls the Adipogenic Differentiation of Human Adipose-derived Mesenchymal Stem Cells
    Authors: Y Luo, H Ji, Y Cao, X Ding, M Li, H Song, S Li, C WaTableng, H Wu, J Meng, H Du
    Cell Transplant, 2020;29(0):9636897209344.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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