Human ICAT Antibody (536017)

R&D Systems | Catalog # MAB3016

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry

Cited:

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 536017
View all ICAT Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ICAT
Met1-Gln81
Accession # Q9NSA3

Specificity

Detects human ICAT in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human ICAT Antibody (536017)

ICAT antibody in Human Fibrocystic Breast Tissue by Immunohistochemistry (IHC-P).

ICAT in Human Fibrocystic Breast Tissue.

ICAT was detected in immersion fixed paraffin-embedded sections of human fibrocystic breast tissue using Mouse Anti-Human ICAT Monoclonal Antibody (Catalog # MAB3016) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human ICAT Antibody (536017)

Application
Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human fibrocystic breast tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: ICAT

Human ICAT is a physiological inhibitor of the beta -Catenin/Wnt signaling pathway. It is 9 kDa in size and found in both cytosol and nucleus. The molecule contains an alpha ‑helical N-terminal region (aa 9‑54) and an acidic, extended C-terminal region (aa 55‑78). The alpha -helical region binds to beta -Catenin, displacing CBP/p300, while the acidic C-terminus interacts with the beta -Catenin N-terminus, blocking Tcf interaction. Human ICAT shares 97% aa sequence identity with mouse and bovine ICAT, respectively.

Long Name

Beta-Catenin-Interacting Protein

Alternate Names

CTNNBIP1

Entrez Gene IDs

56998 (Human)

Gene Symbol

CTNNBIP1

UniProt

Q9NSA3

Additional ICAT Products

Product Documents for Human ICAT Antibody (536017)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human ICAT Antibody (536017)

For research use only

Citations for Human ICAT Antibody (536017)

Customer Reviews for Human ICAT Antibody (536017)

There are currently no reviews for this product. Be the first to review Human ICAT Antibody (536017) and earn rewards!

Have you used Human ICAT Antibody (536017)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies