Human IFN-epsilon Antibody

Catalog # Availability Size / Price Qty
MAB9147-100
MAB9147-SP
IFN-epsilon in Human Endometrial Cancer Tissue.
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Human IFN-epsilon Antibody Summary

Species Reactivity
Human
Specificity
Detects human IFN-epsilon in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 983338
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IFN-epsilon
Leu22-Arg208
Accession # Q86WN2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human IFN-epsilon Monoclonal Antibody (Catalog # MAB91471).

This product is intended for assay development on various assay platforms requiring antibody pairs.

 
Immunohistochemistry
1-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry IFN-epsilon antibody in Human Endometrial Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

IFN-epsilon in Human Endometrial Cancer Tissue. IFN-epsilon was detected in immersion fixed paraffin-embedded sections of human endometrial cancer tissue using Mouse Anti-Human IFN-epsilon Monoclonal Antibody (Catalog # MAB9147) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cancer cell cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-epsilon

Interferon-epsilon (IFNe) is a type I interferon with expression limited to the brain, lung, intestine and reproductive tract. It has been proposed that unlike other type I interferons, IFNe plays a role in mucosal immunity and protects against pathogens including chlamidia, HIV and herpes simplex virus 2. Its expression is upregulated by TNF alpha. Unlike other type-1 interferons, IFNe is also hormonally regulated, and mouse and human IFNe have been shown to have a binding site in their promoters for progesterone receptor. Epithelial cells isolated from uterine endometrium have higher IFNee expression in the proliferative phase, when estrogen levels are highest. IFNe is the only IFN isoform constituitively expressed in HeLa cells.

Long Name
Interferon epsilon
Entrez Gene IDs
338376 (Human); 230405 (Mouse); 298225 (Rat)
Alternate Names
IFNE; IFN-E; IFNE1PRO655; IFNepsilon; IFN-epsilon; IFNT1; Ifn-tau-11; INFE1; interferon epsilon 1; interferon epsilon; Interferon epsilon-1; interferon tau-1; interferon, epsilon; interferon-epsilon; MGC119018; MGC119020

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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