Human IGF-I/IGF-1 Antibody

Catalog # Availability Size / Price Qty
MAB291-SP
MAB291-100
MAB291-500
Human IGF-I/IGF-1 Antibody in Data
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Product Details
Citations (4)
FAQs
Supplemental Products
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Human IGF-I/IGF-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IGF-I/IGF-1 in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant mouse (rm) IGF-I/IGF-1, rmIGF‑II/IGF-2, or recombinant human IGF‑II/IGF-2.
Source
Monoclonal Mouse IgG1 Clone # 56408
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human IGF-I/IGF-1
Gly49-Ala118
Accession # P01343
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Human IGF-I/IGF-1 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human IGF-I/IGF-1 Biotinylated Antibody (Catalog # BAF291)

Standard: Recombinant Human IGF-I/IGF-1 Protein, CF (Catalog # 291-G1)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Recombinant Human IGF-I/IGF-1 by Western Blot. View Larger

Detection of Recombinant Human IGF-I/IGF-1 by Western Blot. Western blot shows 25 ng of Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1), Recombinant Mouse IGF-I/IGF-1 (Catalog # 791-MG), Recombinant Rat IGF-I/IGF-1 (Catalog # 4326-RG), and Recombinant Human IGF-II (Catalog # 292-G2). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human IGF-I/IGF-1 Monoclonal Antibody (Catalog # MAB291) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IGF-I/IGF-1 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGF-I/IGF-1

IGF-I/IGF-1 belongs to the family of insulin-like growth factors and circulates in complex with IGF binding proteins. It is a potent mitogenic growth factor that binds the heteromeric type I and type II IGF receptors. Essentially, all of the biological activities of IGF-I/IGF-1 are mediated by IGF-I R.

Long Name
Insulin-like Growth Factor I/Insulin-like Growth Factor 1
Entrez Gene IDs
3479 (Human); 16000 (Mouse); 24482 (Rat)
Alternate Names
IBP1; IGF1; IGF-1; IGF1A; IGFI; IGF-I; IGF-IA; IGF-IB; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor 1; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; Mechano growth factor; MGF; Somatomedin A; Somatomedin C; somatomedin-C

Product Datasheets

Citations for Human IGF-I/IGF-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Enabling Low Cost Biopharmaceuticals: A Systematic Approach to Delete Proteases from a Well-Known Protein Production Host Trichoderma reesei.
    Authors: Landowski C, Huuskonen A, Wahl R, Westerholm-Parvinen A, Kanerva A, Hanninen A, Salovuori N, Penttila M, Natunen J, Ostermeier C, Helk B, Saarinen J, Saloheimo M
    PLoS ONE, 2015;10(8):e0134723.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  2. ATM regulates insulin-like growth factor 1-secretory clusterin (IGF-1-sCLU) expression that protects cells against senescence.
    Authors: Luo X, Suzuki M, Ghandhi S, Amundson S, Boothman D
    PLoS ONE, 2014;9(6):e99983.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  3. Development and validation of sandwich ELISA microarrays with minimal assay interference.
    Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
    J. Proteome Res., 2008;7(6):2406-14.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Microarray Development
  4. Clinical pharmacodynamic effects of the growth hormone receptor antagonist pegvisomant: implications for cancer therapy.
    Authors: Yin D, Vreeland F, Schaaf LJ, Millham R, Duncan BA, Sharma A
    Clin. Cancer Res., 2007;13(3):1000-9.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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