Human IGF-I/IGF-1 Antibody Summary
Accession # P01343
Human IGF-I/IGF-1 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Recombinant Human IGF-I/IGF-1 by Western Blot. Western blot shows 25 ng of Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1), Recombinant Mouse IGF-I/IGF-1 (Catalog # 791-MG), Recombinant Rat IGF-I/IGF-1 (Catalog # 4326-RG), and Recombinant Human IGF-II (Catalog # 292-G2). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human IGF-I/IGF-1 Monoclonal Antibody (Catalog # MAB291) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IGF-I/IGF-1 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
IGF-I/IGF-1 belongs to the family of insulin-like growth factors and circulates in complex with IGF binding proteins. It is a potent mitogenic growth factor that binds the heteromeric type I and type II IGF receptors. Essentially, all of the biological activities of IGF-I/IGF-1 are mediated by IGF-I R.
Citations for Human IGF-I/IGF-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 5
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Enabling Low Cost Biopharmaceuticals: A Systematic Approach to Delete Proteases from a Well-Known Protein Production Host Trichoderma reesei.
Authors: Landowski C, Huuskonen A, Wahl R, Westerholm-Parvinen A, Kanerva A, Hanninen A, Salovuori N, Penttila M, Natunen J, Ostermeier C, Helk B, Saarinen J, Saloheimo M
PLoS ONE, 2015;10(8):e0134723.
Sample Types: Protein
Applications: Western Blot
ATM regulates insulin-like growth factor 1-secretory clusterin (IGF-1-sCLU) expression that protects cells against senescence.
Authors: Luo X, Suzuki M, Ghandhi S, Amundson S, Boothman D
PLoS ONE, 2014;9(6):e99983.
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Capture)
Development and validation of sandwich ELISA microarrays with minimal assay interference.
Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
J. Proteome Res., 2008;7(6):2406-14.
Sample Types: Serum
Applications: ELISA Microarray Development
Clinical pharmacodynamic effects of the growth hormone receptor antagonist pegvisomant: implications for cancer therapy.
Authors: Yin D, Vreeland F, Schaaf LJ, Millham R, Duncan BA, Sharma A
Clin. Cancer Res., 2007;13(3):1000-9.
Sample Types: Plasma
Applications: ELISA Development
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