Human IGF-I/IGF-1 Antibody

Name: Human IGF-I/IGF-1 Antibody
Brand: R&D Systems
SKU: AF-291-SP
Rating: 4.5 (2 reviews)

Catalog #: AF-291-NA
26 Citations 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF-291-NA
AF-291-SP

Cell Proliferation Induced by IGF-I/IGF-1 and Neutralization by Human IGF-I/IGF-1 Antibody.

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Citations (26)

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Reviews (2)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IGF-I/IGF-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human IGF-I/IGF-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse IGF-I/IGF-1 and recombinant rat IGF-I/IGF-1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human IGF-I/IGF-1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1)

Simple Western

50 µg/mL

Recombinant Human IGF-1

Immunohistochemistry

5-15 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

See below

Neutralization

Measured by its ability to neutralize IGF-I/IGF-1-induced proliferation in the MCF‑7 human breast cancer cell line. Karey, K.P. et al. (1988) Cancer Research 48:4083. The Neutralization Dose (ND₅₀) is typically 3-12 µg/mL in the presence of 6 ng/mL Recombinant Human IGF-I/IGF-1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by IGF-I/IGF-1 and Neutralization by Human IGF-I/IGF-1 Antibody. Recombinant Human IGF-I/IGF-1 (291-G1) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IGF-I/IGF-1 (6 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA). The ND₅₀ is typically 3-12 µg/mL.

Immunocytochemistry

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IGF-I/IGF-1 in MDA‑MB‑231 Human Cell Line. IGF-I/IGF-1 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry

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IGF-I/IGF-1 in Human Placenta. IGF-I/IGF-1 was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblasts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western

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Detection of Human IGF-I/IGF-1 by Simple Western^TM. Simple Western lane view shows lysates of Recombinant Human IGF-1, loaded at 0.2 mg/mL. A specific band was detected for IGF-I/IGF-1 at approximately 6 kDa (as indicated) using 50 µg/mL of Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA). This experiment was conducted under reducing conditions and using the 2-40kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGF-I/IGF-1

IGF-I/IGF-1 belongs to the family of insulin-like growth factors and circulates in complex with IGF binding proteins. It is a potent mitogenic growth factor that binds the heteromeric type I and type II IGF receptors. Essentially, all of the biological activities of IGF-I/IGF-1 are mediated by IGF-I R.

Long Name

Insulin-like Growth Factor I/Insulin-like Growth Factor 1

Entrez Gene IDs

3479 (Human); 16000 (Mouse); 24482 (Rat)

Alternate Names

IBP1; IGF1; IGF-1; IGF1A; IGFI; IGF-I; IGF-IA; IGF-IB; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor 1; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; Mechano growth factor; MGF; Somatomedin A; Somatomedin C; somatomedin-C

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human IGF-I/IGF-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
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Primary Head and Neck Tumour-Derived Fibroblasts Promote Lymphangiogenesis in a Lymphatic Organotypic Co-culture Model
Authors: KM Lugo-Cintr, JM Ayuso, M Humayun, MM Gong, SC Kerr, SM Ponik, PM Harari, M Virumbrale, DJ Beebe
EBioMedicine, 2021-10-18;73(0):103634.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
lncRNA NR2F1-AS1 promotes breast cancer angiogenesis through activating IGF-1/IGF-1R/ERK pathway
Authors: Q Zhang, T Li, Z Wang, X Kuang, N Shao, Y Lin
J. Cell. Mol. Med., 2020-06-17;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Controlled Release of rAAV Vectors from APMA-Functionalized Contact Lenses for Corneal Gene Therapy
Authors: F Alvarez-Ri, A Rey-Rico, JK Venkatesan, L Diaz-Gomez, M Cucchiarin, A Concheiro, C Alvarez-Lo
Pharmaceutics, 2020-04-09;12(4):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
Molecular Basis of The Retinal Pigment Epithelial Changes That Characterize The Ocular Lesion in Toxoplasmosis
Authors: S Lie, BR Vieira, S Arruda, M Simões, LM Ashander, JM Furtado, JR Smith
Microorganisms, 2019-09-29;7(10):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Therapeutic Effects of rAAV-Mediated Concomittant Gene Transfer and Overexpression of TGF-? and IGF-I on the Chondrogenesis of Human Bone-Marrow-Derived Mesenchymal Stem Cells
Authors: S Morscheid, A Rey-Rico, G Schmitt, H Madry, M Cucchiarin, JK Venkatesan
Int J Mol Sci, 2019-05-27;20(10):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
A Unique Pattern of Mesothelial-Mesenchymal Transition Induced in the Normal Peritoneal Mesothelium by High-Grade Serous Ovarian Cancer
Authors: M Paku?a, P Uruski, A Niklas, A Wo?niak, D Szpurek, A Tykarski, J Miku?a-Pie, K Ksi??ek
Cancers (Basel), 2019-05-13;11(5):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Dopamine neuron-derived IGF-1 controls dopamine neuron firing, skill learning, and exploration
Authors: A Pristerà, C Blomeley, E Lopes, S Threlfell, E Merlini, D Burdakov, S Cragg, F Guillemot, SL Ang
Proc. Natl. Acad. Sci. U.S.A., 2019-02-11;116(9):3817-3826.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
The Epithelial-Mesenchymal Transition Initiated by Malignant Ascites Underlies the Transmesothelial Invasion of Ovarian Cancer Cells
Authors: M Paku?a, J Miku?a-Pie, A Witucka, K Kostka-Jez, P Uruski, R Moszy?ski, E Naumowicz, S Sajdak, A Tykarski, K Ksi??ek
Int J Mol Sci, 2019-01-02;20(1):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
The effect of cinnamon on polycystic ovary syndrome in a mouse model
Authors: L Dou, Y Zheng, L Li, X Gui, Y Chen, M Yu, Y Guo
Reprod. Biol. Endocrinol., 2018-10-19;16(1):99.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Protein C receptor stimulates multiple signaling pathways in breast cancer cells
Authors: D Wang, C Liu, J Wang, Y Jia, X Hu, H Jiang, ZM Shao, YA Zeng
J. Biol. Chem., 2017-12-07;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Adipose stem cells from chronic pancreatitis patients improve mouse and human islet survival and function
Authors: L Song, Z Sun, DS Kim, W Gou, C Strange, H Dong, W Cui, G Gilkeson, KA Morgan, DB Adams, H Wang
Stem Cell Res Ther, 2017-08-30;8(1):192.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization

Peripheral blood aspirates overexpressing IGF-I via rAAV gene transfer undergo enhanced chondrogenic differentiation processes
Authors: J Frisch, P Orth, A Rey-Rico, JK Venkatesan, G Schmitt, H Madry, D Kohn, M Cucchiarin
J. Cell. Mol. Med., 2017-05-03;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC

Chondrogenic Differentiation Processes in Human Bone Marrow Aspirates upon rAAV-Mediated Gene Transfer and Overexpression of the Insulin-Like Growth Factor I
Authors: Magali Cucchiarin
Tissue Eng Part A, 2015-07-22;21(17):2460-71.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

A subset of bone marrow stromal cells regulate ATP-binding cassette gene expression via insulin-like growth factor-I in a leukemia cell line.
Authors: Benabbou N, Mirshahi P, Bordu C, Faussat A, Tang R, Therwath A, Soria J, Marie J, Mirshahi M
Int J Oncol, 2014-07-29;45(4):1372-80.
Species: Human
Sample Types: Whole Cells
Applications: ICC

ATM regulates insulin-like growth factor 1-secretory clusterin (IGF-1-sCLU) expression that protects cells against senescence.
Authors: Luo X, Suzuki M, Ghandhi S, Amundson S, Boothman D
PLoS ONE, 2014-06-17;9(6):e99983.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Detection)

Hypoxia enhances protective effect of placental-derived mesenchymal stem cells on damaged intestinal epithelial cells by promoting secretion of insulin-like growth factor-1.
Authors: Du, Lili, Yu, Yanqiu, Ma, Haiying, Lu, Xiaomei, Ma, Ling, Jin, Yunan, Zhang, Haipeng
Int J Mol Sci, 2014-01-27;15(2):1983-2002.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

IGF-1 promotes the development and cytotoxic activity of human NK cells.
Authors: Ni, Fang, Sun, Rui, Fu, Binqing, Wang, Fuyan, Guo, Chuang, Tian, Zhigang, Wei, Haiming
Nat Commun, 2013-01-01;4(0):1479.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Benefits of recombinant adeno-associated virus (rAAV)-mediated insulinlike growth factor I (IGF-I) overexpression for the long-term reconstruction of human osteoarthritic cartilage by modulation of the IGF-I axis.
Authors: Weimer A, Madry H, Venkatesan JK
Mol. Med., 2012-05-09;18(1):346-58.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot

Growth hormone promotes lymphangiogenesis.
Authors: Banziger-Tobler NE, Halin C, Kajiya K, Detmar M
Am. J. Pathol., 2008-06-26;173(2):586-97.
Species: Human
Sample Types: Cell Culture Supernates, Cell Lysates, Whole Cells
Applications: ELISA Development, Immunoprecipitation, Neutralization, Western Blot

Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor.
Authors: Skibinski G, Elborn JS, Ennis M
Am. J. Physiol. Lung Cell Mol. Physiol., 2007-03-23;293(1):L69-76.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Effect of construct properties on encapsulated chondrocyte expression of insulin-like growth factor-1.
Authors: Yoon DM, Hawkins EC, Francke-Carroll S, Fisher JP
Biomaterials, 2006-09-18;28(2):299-306.
Species: Bovine
Sample Types: Cell Lysates
Applications: Western Blot

Mining the acute respiratory distress syndrome proteome: identification of the insulin-like growth factor (IGF)/IGF-binding protein-3 pathway in acute lung injury.
Authors: Schnapp LM, Donohoe S, Chen J, Sunde DA, Kelly PM, Ruzinski J, Martin T, Goodlett DR
Am. J. Pathol., 2006-07-01;169(1):86-95.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

The role of matrix metalloproteinase-7 in redefining the gastric microenvironment in response to Helicobacter pylori.
Authors: McCaig C, Duval C, Hemers E, Steele I, Pritchard DM, Przemeck S, Dimaline R, Ahmed S, Bodger K, Kerrigan DD, Wang TC, Dockray GJ, Varro A
Gastroenterology, 2006-03-06;130(6):1754-63.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Induced expression of insulin-like growth factor-1 by amniotic membrane-conditioned medium in cultured human corneal epithelial cells.
Authors: Lee JH, Ryu IH, Kim EK, Lee JE, Hong S, Lee HK
Invest. Ophthalmol. Vis. Sci., 2006-03-01;47(3):864-72.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Gene induction during differentiation of human pulmonary type II cells in vitro.
Authors: Wade KC, Guttentag SH, Gonzales LW, Maschhoff KL, Gonzales J, Kolla V, Singhal S, Ballard PL
Am. J. Respir. Cell Mol. Biol., 2006-02-10;34(6):727-37.
Species: Human
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC-Fr, Western Blot

Quantification, localization, and expression of IGF-I and TGF-beta1 during growth factor-stimulated fracture healing.
Authors: Brenner N, Huning M, Stange R
Calcif. Tissue Int., 2004-04-01;74(4):388-97.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-P