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Human IGF-I/IGF-1 Antibody

Catalog #: AF-291-NA
42 Citations 2 Reviews Datasheet / COA / SDS
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AF-291-NA
AF-291-SP

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Cell Proliferation Induced by IGF-I/IGF-1 and Neutralization by Human IGF-I/IGF-1 Antibody.
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Citations (42)
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Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IGF-I/IGF-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IGF-I/IGF-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse IGF-I/IGF-1 and recombinant rat IGF-I/IGF-1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human IGF-I/IGF-1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1)
Simple Western
50 µg/mL
Recombinant Human IGF-1
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize IGF-I/IGF-1-induced proliferation in the MCF‑7 human breast cancer cell line. Karey, K.P. et al. (1988) Cancer Research 48:4083. The Neutralization Dose (ND50) is typically 3-12 µg/mL in the presence of 6 ng/mL Recombinant Human IGF-I/IGF-1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IGF-I/IGF-1 and Neutralization by Human IGF-I/IGF-1 Antibody. View Larger

Cell Proliferation Induced by IGF-I/IGF-1 and Neutralization by Human IGF-I/IGF-1 Antibody. Recombinant Human IGF-I/IGF-1 (291-G1) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IGF-I/IGF-1 (6 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA). The ND50 is typically 3-12 µg/mL.

Immunocytochemistry IGF-I/IGF-1 antibody in MDA-MB-231 Human Cell Line by Immunocytochemistry (ICC). View Larger

IGF-I/IGF-1 in MDA‑MB‑231 Human Cell Line. IGF-I/IGF-1 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry IGF-I/IGF-1 antibody in Human Placenta by Immunohistochemistry (IHC-P). View Larger

IGF-I/IGF-1 in Human Placenta. IGF-I/IGF-1 was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblasts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western View Larger

Detection of Human IGF-I/IGF-1 by Simple WesternTM. Simple Western lane view shows lysates of Recombinant Human IGF-1, loaded at 0.2 mg/mL. A specific band was detected for IGF-I/IGF-1 at approximately 6 kDa (as indicated) using 50 µg/mL of Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA). This experiment was conducted under reducing conditions and using the 2-40kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGF-I/IGF-1

IGF-I/IGF-1 belongs to the family of insulin-like growth factors and circulates in complex with IGF binding proteins. It is a potent mitogenic growth factor that binds the heteromeric type I and type II IGF receptors. Essentially, all of the biological activities of IGF-I/IGF-1 are mediated by IGF-I R.

Long Name
Insulin-like Growth Factor I/Insulin-like Growth Factor 1
Entrez Gene IDs
3479 (Human); 16000 (Mouse); 24482 (Rat)
Alternate Names
IBP1; IGF1; IGF-1; IGF1A; IGFI; IGF-I; IGF-IA; IGF-IB; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor 1; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; Mechano growth factor; MGF; Somatomedin A; Somatomedin C; somatomedin-C

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Citations for Human IGF-I/IGF-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

42 Citations: Showing 1 - 10
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  1. Overexpression of human IGF-I via direct rAAV-mediated gene transfer improves the early repair of articular cartilage defects in vivo.
    Authors: Cucchiarini M, Madry H
    Gene Ther, 2014-07-03;21(9):811-9.
  2. Malignant Ascites Promote Adhesion of Ovarian Cancer Cells to Peritoneal Mesothelium and Fibroblasts
    Authors: P Uruski, J Miku?a-Pie, M Paku?a, S Budkiewicz, M Drzewiecki, AN Gaiday, M Wierzowiec, E Naumowicz, R Moszy?ski, A Tykarski, K Ksi??ek
    International Journal of Molecular Sciences, 2021-04-19;22(8):.
  3. Local autocrine plasticity signaling in single dendritic spines by insulin-like growth factors
    Authors: Tu, X;Jain, A;Parra Bueno, P;Decker, H;Liu, X;Yasuda, R;
    Science advances
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Reversible Effects of Functional Mandibular Lateral Shift on Masticatory Muscles in Growing Rats
    Authors: Guan, H;Yonemitsu, I;Ikeda, Y;Ono, T;
    Biomedicines
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  5. Stress Granules Determine the Development of Obesity-Associated Pancreatic Cancer
    Authors: Guillaume Fonteneau, Alexandra Redding, Hannah Hoag-Lee, Edward S. Sim, Stefan Heinrich, Matthias M. Gaida et al.
    Cancer Discovery
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Primary Head and Neck Tumour-Derived Fibroblasts Promote Lymphangiogenesis in a Lymphatic Organotypic Co-culture Model
    Authors: KM Lugo-Cintr, JM Ayuso, M Humayun, MM Gong, SC Kerr, SM Ponik, PM Harari, M Virumbrale, DJ Beebe
    EBioMedicine, 2021-10-18;73(0):103634.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. lncRNA NR2F1-AS1 promotes breast cancer angiogenesis through activating IGF-1/IGF-1R/ERK pathway
    Authors: Q Zhang, T Li, Z Wang, X Kuang, N Shao, Y Lin
    J. Cell. Mol. Med., 2020-06-17;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Controlled Release of rAAV Vectors from APMA-Functionalized Contact Lenses for Corneal Gene Therapy
    Authors: F Alvarez-Ri, A Rey-Rico, JK Venkatesan, L Diaz-Gomez, M Cucchiarin, A Concheiro, C Alvarez-Lo
    Pharmaceutics, 2020-04-09;12(4):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  9. IGFBP-1 in Cardiometabolic Pathophysiology—Insights From Loss-of-Function and Gain-of-Function Studies in Male Mice
    Authors: Natalie J Haywood, Thomas A Slater, Michael Drozd, Nele Warmke, Connor Matthews, Paul A Cordell et al.
    Journal of the Endocrine Society
  10. Molecular Basis of The Retinal Pigment Epithelial Changes That Characterize The Ocular Lesion in Toxoplasmosis
    Authors: S Lie, BR Vieira, S Arruda, M Simões, LM Ashander, JM Furtado, JR Smith
    Microorganisms, 2019-09-29;7(10):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  11. Remodeling of Human Osteochondral Defects via rAAV-Mediated Co-Overexpression of TGF-beta and IGF-I from Implanted Human Bone Marrow-Derived Mesenchymal Stromal Cells
    Authors: Stephanie Morscheid, Jagadeesh Kumar Venkatesan, Ana Rey-Rico, Gertrud Schmitt, Magali Cucchiarini
    Journal of Clinical Medicine
  12. Therapeutic Effects of rAAV-Mediated Concomittant Gene Transfer and Overexpression of TGF-? and IGF-I on the Chondrogenesis of Human Bone-Marrow-Derived Mesenchymal Stem Cells
    Authors: S Morscheid, A Rey-Rico, G Schmitt, H Madry, M Cucchiarin, JK Venkatesan
    Int J Mol Sci, 2019-05-27;20(10):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  13. A Unique Pattern of Mesothelial-Mesenchymal Transition Induced in the Normal Peritoneal Mesothelium by High-Grade Serous Ovarian Cancer
    Authors: M Paku?a, P Uruski, A Niklas, A Wo?niak, D Szpurek, A Tykarski, J Miku?a-Pie, K Ksi??ek
    Cancers (Basel), 2019-05-13;11(5):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  14. Dopamine neuron-derived IGF-1 controls dopamine neuron firing, skill learning, and exploration
    Authors: A Pristerà, C Blomeley, E Lopes, S Threlfell, E Merlini, D Burdakov, S Cragg, F Guillemot, SL Ang
    Proc. Natl. Acad. Sci. U.S.A., 2019-02-11;116(9):3817-3826.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  15. The Epithelial-Mesenchymal Transition Initiated by Malignant Ascites Underlies the Transmesothelial Invasion of Ovarian Cancer Cells
    Authors: M Paku?a, J Miku?a-Pie, A Witucka, K Kostka-Jez, P Uruski, R Moszy?ski, E Naumowicz, S Sajdak, A Tykarski, K Ksi??ek
    Int J Mol Sci, 2019-01-02;20(1):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  16. The effect of cinnamon on polycystic ovary syndrome in a mouse model
    Authors: L Dou, Y Zheng, L Li, X Gui, Y Chen, M Yu, Y Guo
    Reprod. Biol. Endocrinol., 2018-10-19;16(1):99.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  17. The crosstalk between breast carcinoma-associated fibroblasts and cancer cells promotes RhoA-dependent invasion via IGF-1 and PAI-1
    Authors: Julien Daubriac, Shiwei Han, Jelena Grahovac, Eve Smith, Abdel Hosein, Marguerite Buchanan et al.
    Oncotarget
  18. Protein C receptor stimulates multiple signaling pathways in breast cancer cells
    Authors: D Wang, C Liu, J Wang, Y Jia, X Hu, H Jiang, ZM Shao, YA Zeng
    J. Biol. Chem., 2017-12-07;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  19. Adipose stem cells from chronic pancreatitis patients improve mouse and human islet survival and function
    Authors: L Song, Z Sun, DS Kim, W Gou, C Strange, H Dong, W Cui, G Gilkeson, KA Morgan, DB Adams, H Wang
    Stem Cell Res Ther, 2017-08-30;8(1):192.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  20. Peripheral blood aspirates overexpressing IGF-I via rAAV gene transfer undergo enhanced chondrogenic differentiation processes
    Authors: J Frisch, P Orth, A Rey-Rico, JK Venkatesan, G Schmitt, H Madry, D Kohn, M Cucchiarin
    J. Cell. Mol. Med., 2017-05-03;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  21. IGF-1 protects dopamine neurons against oxidative stress: association with changes in phosphokinases
    Authors: Amina El El Ayadi, Michael J. Zigmond, Amanda D. Smith
    Experimental Brain Research
  22. rAAV-mediated overexpression of sox9, TGF-beta and IGF-I in minipig bone marrow aspirates to enhance the chondrogenic processes for cartilage repair
    Authors: J Frisch, A Rey-Rico, J K Venkatesan, G Schmitt, H Madry, M Cucchiarini
    Gene Therapy
  23. Chondrogenic Differentiation Processes in Human Bone Marrow Aspirates upon rAAV-Mediated Gene Transfer and Overexpression of the Insulin-Like Growth Factor I
    Authors: Magali Cucchiarin
    Tissue Eng Part A, 2015-07-22;21(17):2460-71.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  24. Influence of insulin-like growth factor I overexpression via recombinant adeno-associated vector gene transfer upon the biological activities and differentiation potential of human bone marrow-derived mesenchymal stem cells
    Authors: Janina Frisch, Jagadeesh Kumar Venkatesan, Ana Rey-Rico, Gertrud Schmitt, Henning Madry, Magali Cucchiarini
    Stem Cell Research & Therapy
  25. A subset of bone marrow stromal cells regulate ATP-binding cassette gene expression via insulin-like growth factor-I in a leukemia cell line.
    Authors: Benabbou N, Mirshahi P, Bordu C, Faussat A, Tang R, Therwath A, Soria J, Marie J, Mirshahi M
    Int J Oncol, 2014-07-29;45(4):1372-80.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  26. ATM regulates insulin-like growth factor 1-secretory clusterin (IGF-1-sCLU) expression that protects cells against senescence.
    Authors: Luo X, Suzuki M, Ghandhi S, Amundson S, Boothman D
    PLoS ONE, 2014-06-17;9(6):e99983.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  27. Hypoxia enhances protective effect of placental-derived mesenchymal stem cells on damaged intestinal epithelial cells by promoting secretion of insulin-like growth factor-1.
    Authors: Du, Lili, Yu, Yanqiu, Ma, Haiying, Lu, Xiaomei, Ma, Ling, Jin, Yunan, Zhang, Haipeng
    Int J Mol Sci, 2014-01-27;15(2):1983-2002.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  28. Cartilage constructs engineered from chondrocytes overexpressing IGF-I improve the repair of osteochondral defects in a rabbit model
    Authors: H Madry, Centre of Experimental Orthopaedics, Medical Faculty, Building 37, Saarland University, D-66421 Homburg, Germany, G Kaul, D Zurakowski, G Vunjak-Novakovic, M Cucchiarini
    European Cells and Materials
  29. IGF-1 promotes the development and cytotoxic activity of human NK cells.
    Authors: Ni, Fang, Sun, Rui, Fu, Binqing, Wang, Fuyan, Guo, Chuang, Tian, Zhigang, Wei, Haiming
    Nat Commun, 2013-01-01;4(0):1479.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  30. BRCA1 negatively regulates IGF-1 expression through an estrogen-responsive element-like site
    Authors: H J Kang, Y W Yi, H J Kim, Y B Hong, Y S Seong, I Bae
    Cell Death & Disease
  31. Benefits of recombinant adeno-associated virus (rAAV)-mediated insulinlike growth factor I (IGF-I) overexpression for the long-term reconstruction of human osteoarthritic cartilage by modulation of the IGF-I axis.
    Authors: Weimer A, Madry H, Venkatesan JK
    Mol. Med., 2012-05-09;18(1):346-58.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  32. Enhanced Athletic Performance on Multisite AAV-IGF1 Gene Transfer Coincides with Massive Modification of the Muscle Proteome
    Authors: Antero Macedo, Manuela Moriggi, Michele Vasso, Sara De Palma, Mauro Sturnega, Giorgio Friso et al.
    Human Gene Therapy
  33. Cytokines and Growth Factors Expressed by Human Cutaneous Melanoma
    Authors: Elias G. Elias, Joanne H. Hasskamp, Bhuvnesh K. Sharma
    Cancers (Basel)
  34. FOXO transcription factors and VEGF neutralizing antibody enhance antiangiogenic effects of resveratrol
    Authors: Rakesh K. Srivastava, Terry G. Unterman, Sharmila Shankar
    Molecular and Cellular Biochemistry
  35. Growth hormone promotes lymphangiogenesis.
    Authors: Banziger-Tobler NE, Halin C, Kajiya K, Detmar M
    Am. J. Pathol., 2008-06-26;173(2):586-97.
    Species: Human
    Sample Types: Cell Culture Supernates, Cell Lysates, Whole Cells
    Applications: ELISA Development, Immunoprecipitation, Neutralization, Western Blot
  36. Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor.
    Authors: Skibinski G, Elborn JS, Ennis M
    Am. J. Physiol. Lung Cell Mol. Physiol., 2007-03-23;293(1):L69-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  37. Effect of construct properties on encapsulated chondrocyte expression of insulin-like growth factor-1.
    Authors: Yoon DM, Hawkins EC, Francke-Carroll S, Fisher JP
    Biomaterials, 2006-09-18;28(2):299-306.
    Species: Bovine
    Sample Types: Cell Lysates
    Applications: Western Blot
  38. Mining the acute respiratory distress syndrome proteome: identification of the insulin-like growth factor (IGF)/IGF-binding protein-3 pathway in acute lung injury.
    Authors: Schnapp LM, Donohoe S, Chen J, Sunde DA, Kelly PM, Ruzinski J, Martin T, Goodlett DR
    Am. J. Pathol., 2006-07-01;169(1):86-95.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  39. The role of matrix metalloproteinase-7 in redefining the gastric microenvironment in response to Helicobacter pylori.
    Authors: McCaig C, Duval C, Hemers E, Steele I, Pritchard DM, Przemeck S, Dimaline R, Ahmed S, Bodger K, Kerrigan DD, Wang TC, Dockray GJ, Varro A
    Gastroenterology, 2006-03-06;130(6):1754-63.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  40. Induced expression of insulin-like growth factor-1 by amniotic membrane-conditioned medium in cultured human corneal epithelial cells.
    Authors: Lee JH, Ryu IH, Kim EK, Lee JE, Hong S, Lee HK
    Invest. Ophthalmol. Vis. Sci., 2006-03-01;47(3):864-72.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  41. Gene induction during differentiation of human pulmonary type II cells in vitro.
    Authors: Wade KC, Guttentag SH, Gonzales LW, Maschhoff KL, Gonzales J, Kolla V, Singhal S, Ballard PL
    Am. J. Respir. Cell Mol. Biol., 2006-02-10;34(6):727-37.
    Species: Human
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-Fr, Western Blot
  42. Quantification, localization, and expression of IGF-I and TGF-beta1 during growth factor-stimulated fracture healing.
    Authors: Brenner N, Huning M, Stange R
    Calcif. Tissue Int., 2004-04-01;74(4):388-97.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P

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Reviews for Human IGF-I/IGF-1 Antibody

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Human IGF-I/IGF-1 Antibody
By Hannah Wickham on 03/08/2022
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Pancreas tissue Species: Canine

Used at 1:50 concentration in Casein in PBS overnight at 4 C. HIER was performed for two hours at 75 C in a citrate buffer.

Immunocytochemistry/Immunofluorescence Human IGF-I/IGF-1 Antibody AF-291-NA
Human IGF-I Antibody
By Alisha Freeman on 10/27/2017
Application: Siteclcik antibody biotinylation Sample Tested: B16-F1 mouse melanoma cell line Species: Mouse