Human IGF-I/IGF-1 Antibody

Catalog # Availability Size / Price Qty
AF-291-NA
AF-291-SP
Human IGF-I/IGF-1 Antibody in Data
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Product Details
Citations (22)
FAQs
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Reviews (1)

Human IGF-I/IGF-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IGF-I/IGF-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse IGF-I/IGF-1 and recombinant rat IGF-I/IGF-1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human IGF-I/IGF-1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1)
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize IGF-I/IGF-1-induced proliferation in the MCF‑7 human breast cancer cell line. Karey, K.P. et al. (1988) Cancer Research 48:4083. The Neutralization Dose (ND50) is typically 3-12 µg/mL in the presence of 6 ng/mL Recombinant Human IGF-I/IGF-1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Neutralization Cell Proliferation Induced by IGF-I/IGF-1 and Neutralization by Human IGF-I/IGF-1 Antibody. View Larger

Cell Proliferation Induced by IGF-I/IGF-1 and Neutralization by Human IGF-I/IGF-1 Antibody. Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IGF-I/IGF-1 (6 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA). The ND50is typically 3-12 µg/mL.

Immunocytochemistry IGF-I/IGF-1 in MDA‑MB‑231 Human Cell Line. View Larger

IGF-I/IGF-1 in MDA‑MB‑231 Human Cell Line. IGF-I/IGF-1 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry IGF-I/IGF-1 in Human Placenta. View Larger

IGF-I/IGF-1 in Human Placenta. IGF-I/IGF-1 was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblasts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGF-I/IGF-1

IGF-I/IGF-1 belongs to the family of insulin-like growth factors and circulates in complex with IGF binding proteins. It is a potent mitogenic growth factor that binds the heteromeric type I and type II IGF receptors. Essentially, all of the biological activities of IGF-I/IGF-1 are mediated by IGF-I R.

Long Name
Insulin-like Growth Factor I/Insulin-like Growth Factor 1
Entrez Gene IDs
3479 (Human); 16000 (Mouse); 24482 (Rat)
Alternate Names
IBP1; IGF1; IGF-1; IGF1A; IGFI; IGF-I; IGF-IA; IGF-IB; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor 1; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; Mechano growth factor; MGF; Somatomedin A; Somatomedin C; somatomedin-C

Product Datasheets

Citations for Human IGF-I/IGF-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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  1. Molecular Basis of The Retinal Pigment Epithelial Changes That Characterize The Ocular Lesion in Toxoplasmosis
    Authors: S Lie, BR Vieira, S Arruda, M Simões, LM Ashander, JM Furtado, JR Smith
    Microorganisms, 2019;7(10):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Therapeutic Effects of rAAV-Mediated Concomittant Gene Transfer and Overexpression of TGF-? and IGF-I on the Chondrogenesis of Human Bone-Marrow-Derived Mesenchymal Stem Cells
    Authors: S Morscheid, A Rey-Rico, G Schmitt, H Madry, M Cucchiarin, JK Venkatesan
    Int J Mol Sci, 2019;20(10):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. Dopamine neuron-derived IGF-1 controls dopamine neuron firing, skill learning, and exploration
    Authors: A Pristerà, C Blomeley, E Lopes, S Threlfell, E Merlini, D Burdakov, S Cragg, F Guillemot, SL Ang
    Proc. Natl. Acad. Sci. U.S.A., 2019;116(9):3817-3826.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. The Epithelial-Mesenchymal Transition Initiated by Malignant Ascites Underlies the Transmesothelial Invasion of Ovarian Cancer Cells
    Authors: M Paku?a, J Miku?a-Pie, A Witucka, K Kostka-Jez, P Uruski, R Moszy?ski, E Naumowicz, S Sajdak, A Tykarski, K Ksi??ek
    Int J Mol Sci, 2019;20(1):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. The effect of cinnamon on polycystic ovary syndrome in a mouse model
    Authors: L Dou, Y Zheng, L Li, X Gui, Y Chen, M Yu, Y Guo
    Reprod. Biol. Endocrinol., 2018;16(1):99.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Aberrant expression of NKL homeobox gene HLX in Hodgkin lymphoma
    Authors: S Nagel, C Pommerenke, C Meyer, M Kaufmann, RAF MacLeod, HG Drexler
    Oncotarget, 2018;9(18):14338-14353.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Peripheral blood aspirates overexpressing IGF-I via rAAV gene transfer undergo enhanced chondrogenic differentiation processes
    Authors: J Frisch, P Orth, A Rey-Rico, JK Venkatesan, G Schmitt, H Madry, D Kohn, M Cucchiarin
    J. Cell. Mol. Med., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. Adipose stem cells from chronic pancreatitis patients improve mouse and human islet survival and function
    Authors: L Song, Z Sun, DS Kim, W Gou, C Strange, H Dong, W Cui, G Gilkeson, KA Morgan, DB Adams, H Wang
    Stem Cell Res Ther, 2017;8(1):192.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  9. Protein C receptor stimulates multiple signaling pathways in breast cancer cells
    Authors: D Wang, C Liu, J Wang, Y Jia, X Hu, H Jiang, ZM Shao, YA Zeng
    J. Biol. Chem., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. Chondrogenic Differentiation Processes in Human Bone Marrow Aspirates upon rAAV-Mediated Gene Transfer and Overexpression of the Insulin-Like Growth Factor I
    Authors: Magali Cucchiarin
    Tissue Eng Part A, 2015;21(17):2460-71.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  11. A subset of bone marrow stromal cells regulate ATP-binding cassette gene expression via insulin-like growth factor-I in a leukemia cell line.
    Authors: Benabbou N, Mirshahi P, Bordu C, Faussat A, Tang R, Therwath A, Soria J, Marie J, Mirshahi M
    Int J Oncol, 2014;45(4):1372-80.
  12. Hypoxia enhances protective effect of placental-derived mesenchymal stem cells on damaged intestinal epithelial cells by promoting secretion of insulin-like growth factor-1.
    Authors: Du, Lili, Yu, Yanqiu, Ma, Haiying, Lu, Xiaomei, Ma, Ling, Jin, Yunan, Zhang, Haipeng
    Int J Mol Sci, 2014;15(2):1983-2002.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  13. IGF-1 promotes the development and cytotoxic activity of human NK cells.
    Authors: Ni, Fang, Sun, Rui, Fu, Binqing, Wang, Fuyan, Guo, Chuang, Tian, Zhigang, Wei, Haiming
    Nat Commun, 2013;4(0):1479.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  14. Benefits of recombinant adeno-associated virus (rAAV)-mediated insulinlike growth factor I (IGF-I) overexpression for the long-term reconstruction of human osteoarthritic cartilage by modulation of the IGF-I axis.
    Authors: Weimer A, Madry H, Venkatesan JK
    Mol. Med., 2012;18(1):346-58.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. Growth hormone promotes lymphangiogenesis.
    Authors: Banziger-Tobler NE, Halin C, Kajiya K, Detmar M
    Am. J. Pathol., 2008;173(2):586-97.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  16. Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor.
    Authors: Skibinski G, Elborn JS, Ennis M
    Am. J. Physiol. Lung Cell Mol. Physiol., 2007;293(1):L69-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  17. Mining the acute respiratory distress syndrome proteome: identification of the insulin-like growth factor (IGF)/IGF-binding protein-3 pathway in acute lung injury.
    Authors: Schnapp LM, Donohoe S, Chen J, Sunde DA, Kelly PM, Ruzinski J, Martin T, Goodlett DR
    Am. J. Pathol., 2006;169(1):86-95.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  18. Effect of construct properties on encapsulated chondrocyte expression of insulin-like growth factor-1.
    Authors: Yoon DM, Hawkins EC, Francke-Carroll S, Fisher JP
    Biomaterials, 2006;28(2):299-306.
    Species: Bovine
    Sample Types: Cell Lysates
    Applications: Western Blot
  19. The role of matrix metalloproteinase-7 in redefining the gastric microenvironment in response to Helicobacter pylori.
    Authors: McCaig C, Duval C, Hemers E, Steele I, Pritchard DM, Przemeck S, Dimaline R, Ahmed S, Bodger K, Kerrigan DD, Wang TC, Dockray GJ, Varro A
    Gastroenterology, 2006;130(6):1754-63.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  20. Gene induction during differentiation of human pulmonary type II cells in vitro.
    Authors: Wade KC, Guttentag SH, Gonzales LW, Maschhoff KL, Gonzales J, Kolla V, Singhal S, Ballard PL
    Am. J. Respir. Cell Mol. Biol., 2006;34(6):727-37.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  21. Induced expression of insulin-like growth factor-1 by amniotic membrane-conditioned medium in cultured human corneal epithelial cells.
    Authors: Lee JH, Ryu IH, Kim EK, Lee JE, Hong S, Lee HK
    Invest. Ophthalmol. Vis. Sci., 2006;47(3):864-72.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  22. Quantification, localization, and expression of IGF-I and TGF-beta1 during growth factor-stimulated fracture healing.
    Authors: Brenner N, Huning M, Stange R
    Calcif. Tissue Int., 2004;74(4):388-97.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P

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Reviews for Human IGF-I/IGF-1 Antibody

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Human IGF-I Antibody
By Anonymous on 10/27/2017
Application: Siteclcik antibody biotinylation Sample Tested: B16-F1 mouse melanoma cell line Species: Mouse