Human IL-1 RII Antibody

(2 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human IL-1 RII in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-1 RI, rhIL‑18 R, and recombinant mouse IL-18 R is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 32437
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑1 RII
    Phe14-Glu343 (Glu297Gly)
    Accession # P27930
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human IL‑1 RII (Catalog # 263-2R) under non-reducing conditions only
  • Immunocytochemistry
    8-25 µg/mL
    Immersion fixed human peripheral blood mononuclear cells
  • Neutralization
    Measured by its ability to neutralize IL‑1 RII-mediated inhibition of proliferation in the D10.G4.1 mouse helper T cell line. The Neutralization Dose (ND50) is typically 2-6 µg/mL in the presence of 2 µg/mL Recombinant Human IL‑1 RII, 50 pg/mL Recombinant Human IL‑1 beta /IL‑1F2, and 1.25 µg/mL concanavalin A.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
IL‑1 RII Inhibition of IL‑1 beta /IL‑1F2‑dependent Cell Proliferation and Neutralization by Human IL‑1 RII Antibody. Recombinant Human IL‑1 RII (Catalog # 263-2R) inhibits Recombinant Human IL‑1 beta /
IL‑1F2 (Catalog # 201‑LB) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL‑1 beta /IL‑1F2 (50 pg/mL) activity elicited by Recombinant Human IL‑1 RII (2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑1 RII Monoclonal Antibody (Catalog # MAB263). The ND50 is typically 2‑6 µg/mL in the presence of concanavalin A (1.25 µg/mL).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 RII

Two distinct types of receptors that bind the pleiotropic cytokines IL-1 alpha and IL-1 beta have been described. The IL-1 receptor type I is an 80 kDa transmembrane protein that is expressed predominantly by T cells, fibroblasts and endothelial cells. IL-1 receptor type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence similarity in their extracellular domains. The two receptor types do not heterodimerize in a receptor complex.

An IL-1 receptor accessory protein that can heterodimerize with the type I receptor in the presence of IL-1 alpha or IL-1 beta but not IL-1ra, was identified (1). This type I receptor complex appears to mediate all the known IL-1 biological responses. The receptor type II has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling type I receptor complex.

  • References:
    1. Greenfeder, S. et al. (1995) J. Biol. Chem. 270:13757.
  • Long Name:
    Interleukin 1 Receptor II
  • Entrez Gene IDs:
    7850 (Human); 16178 (Mouse)
  • Alternate Names:
    beta; CD121b antigen; CD121b; IL-1 R beta; IL-1 RII; IL1R2; IL1RBCD121 antigen-like family member B; IL-1R-beta; IL1RII; IL-1RII; IL-1RT2; IL-1RT-2; interleukin 1 receptor, type II; Interleukin-1 receptor beta; MGC47725
Related Research Areas
Citations:

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2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Isolation and characterization of human trophoblast side-population (SP) cells in primary villous cytotrophoblasts and HTR-8/SVneo cell line.
    Authors: Takao T, Asanoma K, Kato K
    PLoS ONE, 2011;6(7):e21990.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Immunolocalization of interleukin-1 receptors in the sarcolemma and nuclei of skeletal muscle in patients with idiopathic inflammatory myopathies.
    Authors: Grundtman C, Salomonsson S, Dorph C, Bruton J, Andersson U, Lundberg IE
    Arthritis Rheum., 2007;56(2):674-87.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
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