Human IL-10 Antibody

(20 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human IL-10 in Western blots. In Western blots, this antibody shows approximately 5% cross-reactivity with recombinant mouse
    IL-10.
  • Source
    Monoclonal Mouse IgG2B Clone # 23738
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human IL‑10
    Ser19-Asn178
    Accession # P22301
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize IL‑10-induced proliferation in the MC/9‑2 mouse mast cell line. Thompson-Snipes, L.  et al. (1991) J. Exp. Med. 173:507. At 2 µg/mL, this antibody will neutralize >60% of the bioactivity due to 5 ng/mL Recombinant Human IL‑10.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human IL‑10 by Western Blot. Western blot shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IL‑10 Monoclonal Antibody (Catalog # MAB217) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL‑10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Cell Proliferation Induced by IL‑10 and Neutralization by Human IL-10 Antibody. Recombinant Human IL‑10 (Catalog # 217-IL) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑10 (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑10 Monoclonal Antibody (Catalog # MAB217). At 5 µg/mL, this antibody will neutralize 60% Recombinant Human IL-10 induced bioactivity.
Detection of Human IL‑10 by Simple WesternTM. Simple Western lane view shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10, loaded at 0.2 mg/mL. A specific band was detected for IL‑10 at approximately 23 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human IL‑10 Monoclonal Antibody (Catalog # MAB217). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-10
Interleukin 10 (IL-10) is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is produced predominantly by Th2 lymphocytes and other activated lymphoid cells.
  • Long Name:
    Interleukin 10
  • Entrez Gene IDs:
    3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral)
  • Alternate Names:
    CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. The therapeutic HIV Env C5/gp41 vaccine candidate Vacc-C5 induces specific T cell regulation in a phase I/II clinical study
    Authors: K Brekke, M Sommerfelt, M Ökvist, AM Dyrhol-Rii, D Kvale
    BMC Infect. Dis, 2017;17(1):228.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  2. Idelalisib and caffeine reduce suppression of T cell responses mediated by activated chronic lymphocytic leukemia cells
    Authors: BD Hock, SA MacPherson, JL McKenzie
    PLoS ONE, 2017;12(3):e0172858.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  3. Opposing regulation of the late phase TNF response by mTORC1-IL-10 signaling and hypoxia in human macrophages
    Sci Rep, 2016;6(0):31959.
    Species: Human
    Sample Type: Whole Cells
    Application: Blocking
  4. Fusion of ubiquitin to HIV gag impairs human monocyte-derived dendritic cell maturation and reduces ability to induce gag T cell responses.
    Authors: Herath S, Benlahrech A, Papagatsias T, Athanasopoulos T, Bouzeboudjen Z, Hervouet C, Klavinskis L, Meiser A, Kelleher P, Dickson G, Patterson S
    PLoS ONE, 2014;9(2):e88327.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Capture
  5. TNF-alpha blockade induces IL-10 expression in human CD4+ T cells.
    Authors: Evans H, Roostalu U, Walter G, Gullick N, Frederiksen K, Roberts C, Sumner J, Baeten D, Gerwien J, Cope A, Geissmann F, Kirkham B, Taams L
    Nat Commun, 2014;5(0):3199.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  6. Autocrine IL-10 promotes human B-cell differentiation into IgM- or IgG-secreting plasmablasts.
    Authors: Heine G, Drozdenko G, Grun J, Chang H, Radbruch A, Worm M
    Eur J Immunol, 2014;44(6):1615-21.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  7. Differential signaling of cmvIL-10 through common variants of the IL-10 receptor 1.
    Authors: Gruber SG, Gloria Luciani M, Grundtner P, Zdanov A, Gasche C
    Eur. J. Immunol., 2008;38(12):3365-75.
    Species: Human
    Sample Type: Recombinant Protein
    Application: WB
  8. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
    Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
    Blood, 2007;109(10):4272-9.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: Luminex Development
  9. Hepatitis C virus (HCV)-specific CD8+ cells produce transforming growth factor beta that can suppress HCV-specific T-cell responses.
    Authors: Alatrakchi N, Graham CS, van der Vliet HJ, Sherman KE, Exley MA, Koziel MJ
    J. Virol., 2007;81(11):5882-92.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  10. Induction of host chemotactic response by Encephalitozoon spp.
    Authors: Fischer J, West J, Agochukwu N, Suire C, Hale-Donze H
    Infect. Immun., 2007;75(4):1619-25.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  11. Human dendritic cells acquire a semimature phenotype and lymph node homing potential through interaction with CD4+CD25+ regulatory T cells.
    Authors: Bayry J, Triebel F, Kaveri SV, Tough DF
    J. Immunol., 2007;178(7):4184-93.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  12. Increased frequency of CD4+ cells expressing CD161 in cancer patients.
    Authors: Iliopoulou EG, Karamouzis MV, Missitzis I, Ardavanis A, Sotiriadou NN, Baxevanis CN, Rigatos G, Papamichail M, Perez SA
    Clin. Cancer Res., 2006;12(23):6901-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  13. Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.
    Authors: Ebert EC, Mehta V
    Cell. Immunol., 2006;241(1):7-13.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  14. Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function.
    Authors: Godfrey WR, Spoden DJ, Ge YG, Baker SR, Liu B, Levine BL, June CH, Blazar BR, Porter SB
    Blood, 2005;105(2):750-8.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: Neut
  15. Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells.
    Authors: Sen Y, Chunsong H, Baojun H, Linjie Z, Qun L, San J, Qiuping Z, Junyan L, Zhang X, Jinquan T
    Immunology, 2004;112(2):274-89.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  16. Rapid analysis of inflammatory cytokines in cerebrospinal fluid using chip-based immunoaffinity electrophoresis.
    Authors: Phillips TM
    Electrophoresis, 2004;25(10):1652-9.
    Species: Human
    Sample Type: Serum
    Application: Affinity Chromatography
  17. Distinct transcriptional programs activated by interleukin-10 with or without lipopolysaccharide in dendritic cells: induction of the B cell-activating chemokine, CXC chemokine ligand 13.
    Authors: Perrier P, Martinez FO, Locati M, Bianchi G, Nebuloni M, Vago G, Bazzoni F, Sozzani S, Allavena P, Mantovani A
    J. Immunol., 2004;172(11):7031-42.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  18. In vitro-expanded human CD4(+)CD25(+) T-regulatory cells can markedly inhibit allogeneic dendritic cell-stimulated MLR cultures.
    Authors: Godfrey WR, Ge YG, Spoden DJ, Levine BL, June CH, Blazar BR, Porter SB
    Blood, 2004;104(2):453-61.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  19. IL-15 and cognate antigen successfully expand de novo-induced human antigen-specific regulatory CD4+ T cells that require antigen-specific activation for suppression.
    Authors: Koenen HJ, Fasse E, Joosten I
    J. Immunol., 2003;171(12):6431-41.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  20. Regulatory activity of autocrine IL-10 on dendritic cell functions.
    Authors: Corinti S, la Sala A, Pastore S
    J. Immunol., 2001;166(7):4312-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
Expand to show all 20 Citations
Isotype Controls
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Mouse IgG2B Isotype Control

Ctrl MAB004 32  
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Mouse IgG2B Isotype Control

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Mouse IgG2B Isotype Control

Ctrl MAB0042 6
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Primary Antibodies
Description Application Cat# Citations Images  

Human IL-10 Antibody

WB, Neut MAB217R  
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Secondary Antibodies
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Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133 1  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

Flow F0134 1
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Rat Anti-Mouse IgG2B PE-conjugated Antibody

Flow F0132  
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Mouse IgG2B Antibody

Flow MAB0043  
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