Human IL‑10 Antibody

Detection of IL-10 by Immunocytochemistry/ Immunofluorescence

Effects of topo I-reactive B cells on the differentiation of CD4+ T cells in systemic sclerosis (SSc) patients.B cells with low affinity for topo I and those with high affinity for topo I as well as topo I-non-reactive B cells were obtained from anti-topo I antibody-positive SSc patients (n = 111). These B cells were co-cultured with CD4+ T cells. After 48 hr of co-culture, mRNA was extracted from these cells and FoxP3 and ROR gamma t expression levels were examined by real-time RT-PCR. The results were presented when 96-well plates were used as a co-culture site (A) and when microculture plates (B) were used (C). These cells were further co-cultured on microculture plates, and the protein expression of CD4, CD20, FoxP3, and ROR gamma t was confirmed by fluorescent cell staining and signal intensity was determined by ImageJ (D). Similarly, co-culture in microculture plates in the presence of anti-IL-10 (10 μg/ml), anti-IL-35 (5 μg/ml), anti-IL-6 (1 μg/ml), or anti-IL-23 (5 μg/ml) antibodies (Abs) was conducted, followed by fluorescent cell staining (E). These results represented seven experiments. The bar graphs show the mean + SD. Original magnification, ×1000. *p<0.05.Figure 3—source data 1.Source file for the effects of topo I-reactive B cells on the differentiation of CD4+ T cells in systemic sclerosis (SSc) patients.This archive contains all data used for the quantitative analysis shown in Figure 3.Source file for the effects of topo I-reactive B cells on the differentiation of CD4+ T cells in systemic sclerosis (SSc) patients.This archive contains all data used for the quantitative analysis shown in Figure 3. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34854378), licensed under a CC-BY license. Not internally tested by R&D Systems.