Human IL-10 Antibody (25209R)

Name: Human IL-10 Antibody (25209R)
Brand: R&D Systems
SKU: MAB2171R
Price: 399 USD
Availability: InStock

R&D Systems | Catalog # MAB2171R

Submit a review

Product Documents

Recombinant Monoclonal Antibody.

View Available Conjugates

100% Guarantee

Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Neutralization

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG_2B Clone # 25209R

View all IL-10 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IL-10
Leu29-Ala216
Accession # P22301

Specificity

Detects human IL-10 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human IL-10 Antibody (25209R)

Detection of Human IL‑10 by Western Blot.

Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB2171R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL-10 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Cell Proliferation Induced by IL-10 and Neutralization by Human IL-10 Antibody.

Recombinant Human IL-10 (Catalog # 217-IL) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL-10 (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB2171R). The ND₅₀ is typically 0.005-0.03 µg/mL.

Applications for Human IL-10 Antibody (25209R)

Application

Recommended Usage

Western Blot

2 µg/mL
Sample: HEK293 human embryonic kidney cell line transfected with human IL-10

Neutralization

Measured by its ability to neutralize IL‑10-induced proliferation in the MC/9-2 mouse mast cell line. Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507. The Neutralization Dose (ND₅₀) is typically 0.005-0.03 µg/mL in the presence of 5 ng/mL Recombinant Human IL‑10.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IL-10

IL-10 is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is produced predominantly by Th2 lymphocytes and other activated lymphoid cells.

Long Name

Interleukin 10

Alternate Names

CSIF, GVHDS, IL10, IL10A, TGIF

Entrez Gene IDs

3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 102133450 (Cynomolgus Monkey); 100034187 (Equine); 493683 (Feline); 100715618 (Guinea Pig); 2949786 (Viral)