Detects human IL-11 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant mouse IL-11 is observed and no cross-reactivity with recombinant human (rh) IL-6, rhCNTF, rhLIF, rhOSM, rhCLC, or rhCT-1 is observed.
Monoclonal Mouse IgG2A Clone # 22626
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human IL‑11 Pro22-Leu199 Accession # P20809
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
MRC‑5 human embryonic lung fibroblast cell line treated with histamine and Recombinant Human TGF-beta (Catalog # 240-B), fixed with paraformaldehyde, and permeabilized with saponin
Measured by its ability to neutralize IL‑11-induced proliferation in the T11 mouse plasmacytoma cell line. Nordan, R. P. et al. (1987) J. Immunol. 139:813. The Neutralization Dose (ND50) is typically 5-15 µg/mL in the presence of 1 ng/mL Recombinant Human IL‑11.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by IL‑11 and Neutralization by Human IL‑11 Antibody. Recombinant Human IL‑11 (Catalog # 218-IL) stimulates proliferation in the T11 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑11 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑11 Monoclonal Antibody (Catalog # MAB218). The ND50 is typically 5-15 µg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
(IL-11) is a pleiotropic cytokine produced by stromal cells. It binds to a dimeric
receptor complex consisting of the IL-11 Ra
chain and the gp130 chain shared by the receptors for the IL-6 cytokine family.
IL-11 has multiple effects on hematopoietic cells and osteoblasts.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.