Human IL-11 Antibody

(4 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human IL-11 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant mouse IL-11 is observed and no cross-reactivity with recombinant human (rh) IL-6, rhCNTF, rhLIF, rhOSM, rhCLC, or rhCT-1 is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 22626
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human IL‑11
    Pro22-Leu199
    Accession # P20809
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human IL‑11 (Catalog # 218-IL)
  • Neutralization
    Measured by its ability to neutralize IL‑11-induced proliferation in the T11 mouse plasmacytoma cell line. Nordan, R. P. et al. (1987) J. Immunol. 139:813. The Neutralization Dose (ND50) is typically 5-15 µg/mL in the presence of 1 ng/mL Recombinant Human IL‑11.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by IL‑11 and Neutralization by Human IL‑11 Antibody. Recombinant Human IL‑11 (Catalog # 218-IL) stimulates proliferation in the T11 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑11 (1 ng/mL) is neutralized (green line) by increasing concen­trations of Mouse Anti-Human IL‑11 Monoclonal Antibody (Catalog # MAB218). The ND50 is typically 5-15 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-11
Interleukin-11 (IL-11) is a pleiotropic cytokine produced by stromal cells. It binds to a dimeric receptor complex consisting of the IL-11 Ra chain and the gp130 chain shared by the receptors for the IL-6 cytokine family. IL-11 has multiple effects on hematopoietic cells and osteoblasts.
  • Long Name:
    Interleukin 11
  • Entrez Gene IDs:
    3589 (Human); 16156 (Mouse); 171040 (Rat)
  • Alternate Names:
    Adipogenesis inhibitory factor; AGIF; AGIFoprelvekin; IL11; IL-11; IL-11Oprelvekin; interleukin 11; interleukin-11
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. MTA2 enhances colony formation and tumor growth of gastric cancer cells through IL-11.
    Authors: Zhou C, Ji J, Cai Q, Shi M, Chen X, Yu Y, Zhu Z, Zhang J
    BMC Cancer, 2015;15(0):343.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  2. MicroRNA-30c inhibits human breast tumour chemotherapy resistance by regulating TWF1 and IL-11.
    Authors: Bockhorn, Jessica, Dalton, Rachel, Nwachukwu, Chika, Huang, Simo, Prat, Aleix, Yee, Kathy, Chang, Ya-Fang, Huo, Dezheng, Wen, Yujia, Swanson, Kaitlin, Qiu, Tyler, Lu, Jun, Park, Seo Youn, Dolan, M Eileen, Perou, Charles, Olopade, Olufunmi, Clarke, Michael, Greene, Geoffrey, Liu, Huiping
    Nat Commun, 2013;4(0):1393.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  3. Autocrine production of IL-11 mediates tumorigenicity in hypoxic cancer cells.
    Authors: Onnis, Barbara, Fer, Nicole, Rapisarda, Annamari, Perez, Victor S, Melillo, Giovanni
    J Clin Invest, 2013;123(4):1615-29.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  4. IL-11 is required for A1 adenosine receptor-mediated protection against ischemic AKI.
    Authors: Kim J, Kim M, Ham A, Brown K, Greene R, D'Agati V, Lee H
    J Am Soc Nephrol, 213;24(10):1558-70.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A Isotype Control

Ctrl MAB003 32  
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Mouse IgG2A Isotype Control

Ctrl MAB0031 7  
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Mouse IgG2A Isotype Control

Ctrl MAB003R
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse IgG Antibody

WB AF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Rat Anti-Mouse IgG2A PE-conjugated Antibody

Flow F0129 1  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Rat Anti-Mouse IgG2A PerCP-conjugated Antibody

Flow F0131  
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Mouse IgG2A Antibody

Flow MAB0032  
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Rat Anti-Mouse IgG2A APC-conjugated Antibody

Flow F0130  
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