Human IL-17 RA/IL-17 R Antibody

(5 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human IL‑17 RA/IL‑17 R in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse IL‑17 RA/IL‑17 R is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human IL‑17 RA/IL‑17 R
    Leu33-Trp320
    Accession # Q96F46
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Flow Cytometry
    2.5 µg/106 cells
    K562 human chronic myelogenous leukemia cell line
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Neutralization
    Measured by its ability to neutralize IL‑17-induced IL‑6 secretion in normal human dermal fibroblasts. Yao, Z. et al. (1995) Immunity 3:811. The Neutralization Dose (ND50) is typically 0.1-0.5 µg/mL in the presence of 25 ng/mL Recombinant Human IL‑17.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human IL‑17 RA/IL‑17 R by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human IL‑17 RA/IL‑17 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF177) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL‑17 RA/IL‑17 R at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
IL‑6 secretion Induced by IL‑17 and Neutralization by Human IL‑17 RA/IL‑17 R Antibody. Recombinant Human IL‑17 (Catalog # 317-ILB) stimulates IL‑6 secretion in normal human dermal fibroblasts in a dose-dependent manner (orange line), as measured by the Human IL‑6 Quantikine ELISA Kit (Catalog # D6050). IL‑6 secretion elicited by Recombinant Human IL‑17 (25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑17 RA/IL‑17 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF177). The ND50 is typically 0.1-0.5 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-17 RA/IL-17 R

Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleotropic cytokine. IL-17 binds to IL-17 receptor (IL-17 R) which shares no homology with any known family of receptors. While the expression of IL-17 is restricted to activated T cells, the IL-17 R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested. The human IL‑17 R gene was localized to chromosome 22. Human IL-17 R is an 866 amino acid (aa) type I membrane glycoprotein with a 293 aa extracellular domain, a 21 aa carboxy-proximal transmembrane domain, and a 525 aa cytoplasmic tail. The aa sequence of human IL‑17 R is 69% identical to the mouse IL‑17 R. The signaling events of IL-17 includes activation of NF-kappa B and JNK, and require TNF receptor-associated factors 6 (TRAF6) in the signaling pathway.

  • References:
    1. Yao, Z. et al. (1997) Cytokine 9:794.
    2. Schwander, R. et al. (2000) J. Exp. Med. 191:1233.
  • Long Name:
    Interleukin 17 Receptor
  • Entrez Gene IDs:
    23765 (Human); 16172 (Mouse); 312679 (Rat)
  • Alternate Names:
    CD217 antigen; CD217; Cdw217; CDw217interleukin 17 receptor; hIL-17R; IL-17 R; IL-17 RA; IL-17 receptor A; IL17RA; IL-17RA; IL-17RAMGC10262; IL17Rinterleukin-17 receptor A; interleukin 17 receptor A
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Intratumor IL-17-positive mast cells are the major source of the IL-17 that is predictive of survival in gastric cancer patients.
    Authors: Liu, Xiaosun, Jin, Hailong, Zhang, Geer, Lin, Xianke, Chen, Chao, Sun, Jianyi, Zhang, Yu, Zhang, Qing, Yu, Jiren
    PLoS ONE, 2014;9(9):e106834.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  2. Functional coexpression of Interleukin (IL)-7 and its receptor (IL-7R) on Hodgkin and Reed-Sternberg cells: Involvement of IL-7 in tumor cell growth and microenvironmental interactions of Hodgkin's lymphoma.
    Authors: Cattaruzza L, Gloghini A, Olivo K, Di Francia R, Lorenzon D, De Filippi R, Carbone A, Colombatti A, Pinto A, Aldinucci D
    Int. J. Cancer, 2009;125(5):1092-101.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. The human IL-17F/IL-17A heterodimeric cytokine signals through the IL-17RA/IL-17RC receptor complex.
    Authors: Wright JF, Bennett F, Li B, Brooks J, Luxenberg DP, Whitters MJ, Tomkinson KN, Fitz LJ, Wolfman NM, Collins M, Dunussi-Joannopoulos K, Chatterjee-Kishore M, Carreno BM
    J. Immunol., 2008;181(4):2799-805.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. Identification of functional roles for both IL-17RB and IL-17RA in mediating IL-25-induced activities.
    Authors: Rickel EA, Siegel LA, Yoon BR, Rottman JB, Kugler DG, Swart DA, Anders PM, Tocker JE, Comeau MR, Budelsky AL
    J. Immunol., 2008;181(6):4299-310.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  5. IL-17 markedly up-regulates beta-defensin-2 expression in human airway epithelium via JAK and NF-kappaB signaling pathways.
    Authors: Kao CY, Chen Y, Thai P, Wachi S, Huang F, Kim C, Harper RW, Wu R
    J. Immunol., 2004;173(5):3482-91.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

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Goat IgG HRP-conjugated Antibody

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Goat IgG (H+L) PE-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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