Detects human IL‑17 RA/IL‑17 R in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse IL‑17 RA/IL‑17 R is observed.
Polyclonal Goat IgG
Mouse myeloma cell line NS0-derived recombinant human IL‑17 RA/IL‑17 R Leu33-Trp320 Accession # Q96F46
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
2.5 µg/106 cells
K562 human chronic myelogenous leukemia cell line
Measured by its ability to neutralize IL‑17-induced IL‑6 secretion in normal human dermal fibroblasts. Yao, Z. et al. (1995) Immunity 3:811. The Neutralization Dose (ND50) is typically 0.1-0.5 µg/mL in the presence of 25 ng/mL Recombinant Human IL‑17.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human IL‑17 RA/IL‑17 R by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human IL‑17 RA/IL‑17 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF177) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL‑17 RA/IL‑17 R at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
IL‑6 secretion Induced by IL‑17 and Neutralization by Human IL‑17 RA/IL‑17 R Antibody. Recombinant Human IL‑17 (Catalog # 317-ILB) stimulates IL‑6 secretion in normal human dermal fibroblasts in a dose-dependent manner (orange line), as measured by the Human IL‑6 Quantikine ELISA Kit (Catalog # D6050). IL‑6 secretion elicited by Recombinant Human IL‑17 (25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑17 RA/IL‑17 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF177). The ND50 is typically 0.1-0.5 µg/mL.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-17 RA/IL-17 R
Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleotropic cytokine. IL-17 binds to IL-17 receptor (IL-17 R) which shares no homology with any known family of receptors. While the expression of IL-17 is restricted to activated T cells, the IL-17 R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested. The human IL‑17 R gene was localized to chromosome 22. Human IL-17 R is an 866 amino acid (aa) type I membrane glycoprotein with a 293 aa extracellular domain, a 21 aa carboxy-proximal transmembrane domain, and a 525 aa cytoplasmic tail. The aa sequence of human IL‑17 R is 69% identical to the mouse IL‑17 R. The signaling events of IL-17 includes activation of NF-kappa B and JNK, and require TNF receptor-associated factors 6 (TRAF6) in the signaling pathway.
Yao, Z. et al. (1997) Cytokine 9:794.
Schwander, R. et al. (2000) J. Exp. Med. 191:1233.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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