Human IL-17C Antibody

Catalog # Availability Size / Price Qty
MAB1234
MAB1234-SP
IL‑17C in Human Skin.
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Product Details
Citations (1)
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Human IL-17C Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-17C in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human IL-17A, B, D, E or F is observed.
Source
Monoclonal Mouse IgG2A Clone # 177114
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IL‑17C
His19-Val197
Accession # Q9P0M4.1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human IL‑17C (Catalog # 1234-IL)
Immunohistochemistry
5-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Human IL-17C Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human IL‑17C Biotinylated Antibody (Catalog # BAF1234)

Standard: Recombinant Human IL-17C Protein (Catalog # 1234-IL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry IL-17C antibody in Human Skin by Immunohistochemistry (IHC-P). View Larger

IL‑17C in Human Skin. IL-17C was detected in immersion fixed paraffin-embedded sections of human psoriatic skin using Mouse Anti-Human IL-17C Monoclonal Antibody (Catalog # MAB1234) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to keratinocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Intracellular Staining by Flow Cytometry Detection of IL‑17C antibody in PC‑3 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of IL‑17C in PC‑3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Mouse Anti-Human IL-17C Monoclonal Antibody (Catalog # MAB1234, filled histogram) or isotype control antibody (MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17C

The Interleukin-17 (IL-17) family proteins, comprising six members (IL-17, IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cysteine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus (1, 2). With the exception of IL-17B, which exists as a non‑covalently linked dimer, all IL‑17 family members are disulfide-linked dimers (3). IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2). Two receptors (IL-17 R, and IL-17B R), which are activated by IL-17 family members, have been identified. In addition, at least three additional orphan type I transmembrane receptors with homology to IL-17 R, including IL-17 RL (IL-17 RC), IL-17 RD, and IL‑17 RE, have also been reported (1‑4). Human IL-17C cDNA encodes a 197 amino acid (aa) residues protein with a putative 18 aa signal peptide (5). IL-17C shares from 15%‑30% aa sequence identity with other IL-17 family members. Human and mouse IL-17C also share 83% aa sequence identity. IL-17C has a very restricted expression pattern and was detected as a rare expressed sequence tag (EST) sequence in an adult prostate and fetal kidney libraries (2). IL-17C has been shown to stimulate the release of TNF-alpha and IL-1 beta from the monocytic cell line THP-1, a property it shares with IL-17B (5, 6).

References
  1. Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
  2. Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155.
  3. Hymowitz, S.G. et al. (2001) EMBO J. 20:5332.
  4. Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309.
  5. Li, H. et al. (2000) Proc. Natl. Acad. Sci. USA 97:773.
  6. Shi, Y. et al. (2000) J. Biol. Chem. 275:19167.
Long Name
Interleukin 17C
Entrez Gene IDs
27189 (Human); 234836 (Mouse)
Alternate Names
CX2; Cytokine CX2; IL17C; IL-17C; IL-17CMGC126884; interleukin 17C; interleukin-17C; MGC138401

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Citation for Human IL-17C Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Unveiling the Ability of Witch Hazel (Hamamelis virginiana L.) Bark Extract to Impair Keratinocyte Inflammatory Cascade Typical of Atopic Eczema
    Authors: S Piazza, G Martinelli, A Magnavacca, M Fumagalli, C Pozzoli, M Terno, L Canilli, M Angarano, N Maranta, M Dell'Agli, E Sangiovann
    International Journal of Molecular Sciences, 2022-08-17;23(16):.
    Species: Human
    Sample Types: Cell Culture Superantes
    Applications: ELISA Capture

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