Human IL-17RA/IL-17R Antibody

Catalog # Availability Size / Price Qty
Detection of Human IL‑17 RA/IL‑17 R by Western Blot.
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Product Details
Citations (8)
Supplemental Products

Human IL-17RA/IL-17R Antibody Summary

Species Reactivity
Detects human IL‑17 RA/IL‑17 R in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse IL‑17 RA/IL‑17 R is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant human IL‑17 RA/IL‑17 R
Accession # Q96F46
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.


Recommended Concentration
Western Blot
0.5 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
K562 human chronic myelogenous leukemia cell line
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Measured by its ability to neutralize IL‑17-induced IL‑6 secretion in normal human dermal fibroblasts. Yao, Z. et al. (1995) Immunity 3:811. The Neutralization Dose (ND50) is typically 0.1-0.5 µg/mL in the presence of 25 ng/mL Recombinant Human IL‑17.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human IL-17 RA/IL-17 R antibody by Western Blot. View Larger

Detection of Human IL‑17 RA/IL‑17 R by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human IL-17 RA/IL-17 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF177) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL-17 RA/IL-17 R at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Neutralization IL‑6 secretion Induced by IL‑17 and Neutralization by Human IL‑17 RA/IL‑17 R Antibody. View Larger

IL‑6 secretion Induced by IL‑17 and Neutralization by Human IL‑17 RA/IL‑17 R Antibody. Recombinant Human IL-17 (Catalog # 317-ILB) stimulates IL-6 secretion in normal human dermal fibroblasts in a dose-dependent manner (orange line), as measured by the Human IL-6 Quantikine ELISA Kit (Catalog # D6050). IL-6 secretion elicited by Recombinant Human IL-17 (25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-17 RA/IL-17 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF177). The ND50 is typically 0.1-0.5 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17RA/IL-17R

Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleotropic cytokine. IL-17 binds to IL-17 receptor (IL-17 R) which shares no homology with any known family of receptors. While the expression of IL-17 is restricted to activated T cells, the IL-17 R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested. The human IL‑17 R gene was localized to chromosome 22. Human IL-17 R is an 866 amino acid (aa) type I membrane glycoprotein with a 293 aa extracellular domain, a 21 aa carboxy-proximal transmembrane domain, and a 525 aa cytoplasmic tail. The aa sequence of human IL‑17 R is 69% identical to the mouse IL‑17 R. The signaling events of IL-17 includes activation of NF-kappa B and JNK, and require TNF receptor-associated factors 6 (TRAF6) in the signaling pathway.

  1. Yao, Z. et al. (1997) Cytokine 9:794.
  2. Schwander, R. et al. (2000) J. Exp. Med. 191:1233.
Long Name
Interleukin 17 Receptor
Entrez Gene IDs
23765 (Human); 16172 (Mouse); 312679 (Rat); 486759 (Canine); 101925679 (Cynomolgus Monkey)
Alternate Names
CD217 antigen; CD217; Cdw217; CDw217interleukin 17 receptor; hIL-17R; IL-17 R; IL-17 RA; IL-17 receptor A; IL17RA; IL-17RA; IL-17RAMGC10262; IL17Rinterleukin-17 receptor A; interleukin 17 receptor A

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Citations for Human IL-17RA/IL-17R Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Impaired IL-17 Signaling Pathway Contributes to the Increased Collagen Expression in Scleroderma Fibroblasts
    Authors: Taiji Nakashima, Masatoshi Jinnin, Keitaro Yamane, Noritoshi Honda, Ikko Kajihara, Takamitsu Makino et al.
    The Journal of Immunology
  2. IL-17 Promotes p38 MAPK-Dependent Endothelial Activation Enhancing Neutrophil Recruitment to Sites of Inflammation
    Authors: Lucie Roussel, François Houle, Carlos Chan, Yu Yao, Julie Bérubé, Ron Olivenstein et al.
    The Journal of Immunology
  3. A bi-specific inhibitor targeting IL-17A and MMP-9 reduces invasion and motility in MDA-MB-231 cells
    Authors: D Koslawsky, M Zaretsky, R Alcalay, O Mazor, A Aharoni, N Papo
    Oncotarget, 2018-06-19;9(47):28500-28513.
    Species: Human
    Sample Types: Protein
  4. Intratumor IL-17-positive mast cells are the major source of the IL-17 that is predictive of survival in gastric cancer patients.
    Authors: Liu, Xiaosun, Jin, Hailong, Zhang, Geer, Lin, Xianke, Chen, Chao, Sun, Jianyi, Zhang, Yu, Zhang, Qing, Yu, Jiren
    PLoS ONE, 2014-09-08;9(9):e106834.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  5. Functional coexpression of Interleukin (IL)-7 and its receptor (IL-7R) on Hodgkin and Reed-Sternberg cells: Involvement of IL-7 in tumor cell growth and microenvironmental interactions of Hodgkin's lymphoma.
    Authors: Cattaruzza L, Gloghini A, Olivo K, Di Francia R, Lorenzon D, De Filippi R, Carbone A, Colombatti A, Pinto A, Aldinucci D
    Int. J. Cancer, 2009-09-01;125(5):1092-101.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Identification of functional roles for both IL-17RB and IL-17RA in mediating IL-25-induced activities.
    Authors: Rickel EA, Siegel LA, Yoon BR, Rottman JB, Kugler DG, Swart DA, Anders PM, Tocker JE, Comeau MR, Budelsky AL
    J. Immunol., 2008-09-15;181(6):4299-310.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. The human IL-17F/IL-17A heterodimeric cytokine signals through the IL-17RA/IL-17RC receptor complex.
    Authors: Wright JF, Bennett F, Li B, Brooks J, Luxenberg DP, Whitters MJ, Tomkinson KN, Fitz LJ, Wolfman NM, Collins M, Dunussi-Joannopoulos K, Chatterjee-Kishore M, Carreno BM
    J. Immunol., 2008-08-15;181(4):2799-805.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. IL-17 markedly up-regulates beta-defensin-2 expression in human airway epithelium via JAK and NF-kappaB signaling pathways.
    Authors: Kao CY, Chen Y, Thai P, Wachi S, Huang F, Kim C, Harper RW, Wu R
    J. Immunol., 2004-09-01;173(5):3482-91.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization


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