Human IL-17RA/IL-17R Antibody Summary
Accession # Q96F46
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
IL‑6 secretion Induced by IL‑17 and Neutralization by Human IL‑17 RA/IL‑17 R Antibody. Recombinant Human IL-17 (Catalog # 317-ILB) stimulates IL-6 secretion in normal human dermal fibroblasts in a dose-dependent manner (orange line), as measured by the Human IL-6 Quantikine ELISA Kit (Catalog # D6050). IL-6 secretion elicited by Recombinant Human IL-17 (25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-17 RA/IL-17 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF177). The ND50 is typically 0.1-0.5 µg/mL.
Detection of Human IL‑17 RA/IL‑17 R by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human IL-17 RA/IL-17 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF177) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL-17 RA/IL-17 R at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleotropic cytokine. IL-17 binds to IL-17 receptor (IL-17 R) which shares no homology with any known family of receptors. While the expression of IL-17 is restricted to activated T cells, the IL-17 R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested. The human IL‑17 R gene was localized to chromosome 22. Human IL-17 R is an 866 amino acid (aa) type I membrane glycoprotein with a 293 aa extracellular domain, a 21 aa carboxy-proximal transmembrane domain, and a 525 aa cytoplasmic tail. The aa sequence of human IL‑17 R is 69% identical to the mouse IL‑17 R. The signaling events of IL-17 includes activation of NF-kappa B and JNK, and require TNF receptor-associated factors 6 (TRAF6) in the signaling pathway.
Citations for Human IL-17RA/IL-17R Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 6
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A bi-specific inhibitor targeting IL-17A and MMP-9 reduces invasion and motility in MDA-MB-231 cells
Authors: D Koslawsky, M Zaretsky, R Alcalay, O Mazor, A Aharoni, N Papo
Sample Types: Protein
Intratumor IL-17-positive mast cells are the major source of the IL-17 that is predictive of survival in gastric cancer patients.
Authors: Liu, Xiaosun, Jin, Hailong, Zhang, Geer, Lin, Xianke, Chen, Chao, Sun, Jianyi, Zhang, Yu, Zhang, Qing, Yu, Jiren
PLoS ONE, 2014;9(9):e106834.
Sample Types: Whole Tissue
Functional coexpression of Interleukin (IL)-7 and its receptor (IL-7R) on Hodgkin and Reed-Sternberg cells: Involvement of IL-7 in tumor cell growth and microenvironmental interactions of Hodgkin's lymphoma.
Authors: Cattaruzza L, Gloghini A, Olivo K, Di Francia R, Lorenzon D, De Filippi R, Carbone A, Colombatti A, Pinto A, Aldinucci D
Int. J. Cancer, 2009;125(5):1092-101.
Sample Types: Whole Cells
Applications: Flow Cytometry
The human IL-17F/IL-17A heterodimeric cytokine signals through the IL-17RA/IL-17RC receptor complex.
Authors: Wright JF, Bennett F, Li B, Brooks J, Luxenberg DP, Whitters MJ, Tomkinson KN, Fitz LJ, Wolfman NM, Collins M, Dunussi-Joannopoulos K, Chatterjee-Kishore M, Carreno BM
J. Immunol., 2008;181(4):2799-805.
Sample Types: Cell Lysates
Applications: Western Blot
Identification of functional roles for both IL-17RB and IL-17RA in mediating IL-25-induced activities.
Authors: Rickel EA, Siegel LA, Yoon BR, Rottman JB, Kugler DG, Swart DA, Anders PM, Tocker JE, Comeau MR, Budelsky AL
J. Immunol., 2008;181(6):4299-310.
Sample Types: Whole Cells
IL-17 markedly up-regulates beta-defensin-2 expression in human airway epithelium via JAK and NF-kappaB signaling pathways.
Authors: Kao CY, Chen Y, Thai P, Wachi S, Huang F, Kim C, Harper RW, Wu R
J. Immunol., 2004;173(5):3482-91.
Sample Types: Whole Cells
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