Human IL-1 beta /IL-1F2 Propeptide Antibody

Catalog # Availability Size / Price Qty
MAB6964
MAB6964-SP
Detection of Human IL‑1 beta /IL‑1F2 Propeptide by Western Blot.
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Product Details
Citations (6)
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Human IL-1 beta /IL-1F2 Propeptide Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-1 beta /IL-1F2 Propeptide in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-1 alpha, rhIL-1F7, rhIL-1F10, rhIL-18, rhIL-36 alpha, rhIL-36 beta, IL-36 gamma, rhIL-36Ra, recombinant mouse IL-1Ra, or the mature peptides of recombinant IL-1 beta from canine, cotton rat, equine, feline, guinea pig, human, mouse, porcine, rabbit, rat, or rhesus is observed.
Source
Monoclonal Mouse IgG1 Clone # 615417
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IL-1 beta /IL-1F2 Propeptide
Met1-Asp116
Accession # P01584
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human IL-1 beta /IL-1F2 Propeptide antibody by Western Blot. View Larger

Detection of Human IL‑1 beta /IL‑1F2 Propeptide by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 80 nM PMA for 48 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IL-1 beta /IL-1F2 Propeptide Monoclonal Antibody (Catalog # MAB6964) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-1 beta /IL-1F2 Propeptide at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunocytochemistry IL-1 beta /IL-1F2 antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

IL‑1 beta /IL‑1F2 in Human PBMCs. IL-1 beta /IL-1F2 was detected in immersion fixed human peripheral blood mononuclear cells using Mouse Anti-Human IL-1 beta /IL-1F2 Propeptide Monoclonal Antibody (Catalog # MAB6964) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI(blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Simple Western Detection of Human IL-1 beta /IL-1F2 Propeptide antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human IL‑1 beta /IL‑1F2 Propeptide by Simple WesternTM. Simple Western lane view shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with PMA and LPS, loaded at 0.2 mg/mL. A specific band was detected for IL‑1 beta /IL‑1F2 Propeptide at approximately 40 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human IL‑1 beta /IL‑1F2 Propeptide Monoclonal Antibody (Catalog # MAB6964). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 beta/IL-1F2

IL-1 beta, also known as IL1F2, is a prototypical member of the IL-1 superfamily. It is produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. The biological activity of IL-1 beta is mediated by the heterodimeric receptor complex containing IL-1 R1 (IL-1 RI) and IL-1 R3 (IL-1 RAcP). The IL-1 beta propeptide is cleaved intracellularly by caspase-1/ICE to generate a 17 kDa active cytokine. The propeptide of human IL-1 beta shares approximately 55% aa sequence identity with the propeptides of mouse and rat IL-1 beta.

Long Name
Interleukin 1 beta
Entrez Gene IDs
3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Alternate Names
catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta

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Citations for Human IL-1 beta /IL-1F2 Propeptide Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Levistilide A Ameliorates NLRP3 Expression Involving the Syk-p38/JNK Pathway and Peripheral Obliterans in Rats
    Authors: H Guo, L Sun, S Ling, JW Xu
    Mediators Inflamm., 2018-08-12;2018(0):7304096.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Azithromycin decreases NALP3 mRNA stability in monocytes to limit inflammasome-dependent inflammation
    Authors: EA Lendermon, TA Coon, JS Bednash, NM Weathingto, JF McDyer, RK Mallampall
    Respir. Res., 2017-06-28;18(1):131.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. MicroRNA-9 Inhibits NLRP3 Inflammasome Activation in Human Atherosclerosis Inflammation Cell Models through the JAK1/STAT Signaling Pathway
    Authors: Y Wang, Z Han, Y Fan, J Zhang, K Chen, L Gao, H Zeng, J Cao, C Wang
    Cell. Physiol. Biochem, 2017-03-27;41(4):1555-1571.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  4. NLRP3 inflammasome: a novel link between lipoproteins and atherosclerosis
    Arch Med Sci, 2016-07-20;12(5):950-958.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  5. Unsaturated fatty acids prevent activation of NLRP3 inflammasome in human monocytes/macrophages.
    Authors: L'homme L, Esser N, Riva L, Scheen A, Paquot N, Piette J, Legrand-Poels S
    J Lipid Res, 2013-09-03;54(11):2998-3008.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Propionibacterium acnes Induces IL-1beta secretion via the NLRP3 inflammasome in human monocytes.
    Authors: Qin M, Pirouz A, Kim M, Krutzik S, Garban H, Kim J
    J Invest Dermatol, 2013-07-24;134(2):381-8.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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