Human IL-3R alpha Antibody (32703)

R&D Systems | Catalog # MAB301

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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 32703
View all IL-3R alpha Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑3 R alpha /CD123
Lys20-Arg305, predicted
Accession # P26951

Specificity

Detects human IL‑3 R alpha /CD123 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human IL-3R alpha Antibody (32703)

IL-3 Ra/CD123 antibody in Human Peripheral Blood Lymphocytes by Immunocytochemistry (ICC).

IL‑3 R alpha /CD123 in Human Peripheral Blood Lymphocytes.

IL‑3 R alpha /CD123 was detect-ed in immersion fixed human peripheral blood lymphocytes using 2 µg/mL Mouse Anti-Human IL‑3 R alpha /CD123 Monoclonal Antibody (Catalog # MAB301) for 3 hours at room temperature. Cells were stained (red) and counter-stained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
IL-3 Ra/CD123 antibody in Human Tonsil by Immunohistochemistry (IHC-P).

IL‑3 R alpha /CD123 in Human Tonsil.

IL-3 Ra/CD123 was detected in immersion fixed paraffin-embedded sections of human tonsil using 15 µg/mL Mouse Anti-Human IL-3 Ra/CD123 Monoclonal Antibody (Catalog # MAB301) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; CTS002) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Cell Proliferation Induced by IL‑3 and Neutralization by Human IL‑3 R alpha /CD123 Antibody.

Cell Proliferation Induced by IL‑3 and Neutralization by Human IL‑3 R alpha /CD123 Antibody.

Recombinant Human IL-3 (203-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-3 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-3 Ra/CD123 Monoclonal Antibody (Catalog # MAB301). The ND50 is typically 0.6-1.2 µg/mL.

Detection of IL-3R alpha/CD123 in THP-1 cells by Flow Cytometry.

THP-1 cells were stained with Mouse Anti-Human IL-3R alpha/CD123 Monoclonal Antibody (Catalog # MAB301, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human IL-3R alpha Antibody (32703)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: THP‑1 human acute monocytic leukemia cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human peripheral blood lymphocytes

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human tonsil

Western Blot

1 µg/mL
Sample: Recombinant Human IL‑3 R alpha /CD123 (Catalog # 301-R3) under non-reducing conditions only

Neutralization

Measured by its ability to neutralize IL‑3-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.6-1.2 µg/mL in the presence of 0.5 ng/mL Recombinant Human IL‑3.

Reviewed Applications

Read 4 reviews rated 4 using MAB301 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-3R alpha

IL-3 is a pleiotropic cytokine that can stimulate proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors (1, 2). IL-3 exerts its activity through binding to a specific cell surface receptor known as IL-3 R. IL-3 R is a heterodimeric structure composed of a 70 kDa IL-3 R alpha subunit (CD123) and a 120-140 kDa IL-3 R beta subunit (CD131) (3, 4). IL-3 R alpha binds IL-3 with relatively low affinity. In the presence of IL-3 R beta, however, IL-3 R alpha has a much higher affinity for IL-3. It is not clear how signal transduction occurs following IL-3 binding. The IL-3 R alpha chain has a very short intracellular domain while the IL‑3 R beta chain has a very large cytoplasmic domain. The IL‑3 R beta chain is also shared by the receptors for IL-5 and GM-CSF. Cells known to express IL-3 receptors include hematopoietic progenitors, epithelial cells, double negative T cells, mast cells, basophils and blood monocytes (5).

References

  1. Moore, M.A.S. et al. (1991) Blood 72:944.
  2. Warren, D.J. et al. (1988) J. Immunol. 140:94.
  3. Plant M. et al. (1989) Nature 339:150.
  4. Budel, L.M. et al. (1990) Blood 75:1439.
  5. Schrader, J.W. et al. (1988) In Interleukin-3: The Panspecific hemopoietin (ed. J.W. Schrader), Academic Press, San Diego, CA.

Long Name

Interleukin 3 Receptor alpha

Alternate Names

CD123, IL-3 R alpha, IL-3Ra, IL3R alpha, IL3RA

Entrez Gene IDs

3563 (Human); 16188 (Mouse); 102138639 (Cynomolgus Monkey)

Gene Symbol

IL3RA

UniProt

P26951

Additional IL-3R alpha Products

Product Documents for Human IL-3R alpha Antibody (32703)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IL-3R alpha Antibody (32703)

For research use only

Citations for Human IL-3R alpha Antibody (32703)

Customer Reviews for Human IL-3R alpha Antibody (32703) (4)

4 out of 5
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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • Human IL-3R alpha /CD123 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 06/10/2020
    Human IL-3R alpha Antibody (32703) MAB301
  • Human IL-3 R alpha /CD123 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/20/2018
  • Human IL-3 R alpha /CD123 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/07/2018
  • Name: Mehdi Alavi
    Application: Immunohistochemistry
    Sample Tested: DX-3 human melanoma cell line
    Species: Human
    Verified Customer | Posted 03/28/2018

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Protocols

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