Human IL-4 Antibody

Catalog # Availability Size / Price Qty
MAB304-SP
MAB304-100
MAB304-500

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IL‑4 in Human PBMCs.
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Product Details
Citations (7)
FAQs
Supplemental Products
Reviews (1)

Human IL-4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-4 in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse IL‑4.
Source
Monoclonal Mouse IgG1 Clone # 3007
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human IL‑4
His25-Ser153
Accession # P05112.1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human IL‑4 (Catalog # 204-IL)
Immunocytochemistry
8-25 µg/mL
See below
Neutralization
Measured by its ability to neutralize IL‑4-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.03-0.1 µg/mL in the presence of 0.5 ng/mL Recombinant Human IL‑4.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry IL-4 antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

IL‑4 in Human PBMCs. IL-4 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PHA using Mouse Anti-Human IL-4 Monoclonal Antibody (Catalog # MAB304) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Neutralization Cell Proliferation Induced by IL‑4 and Neutralization by Human IL‑4 Antibody. View Larger

Cell Proliferation Induced by IL‑4 and Neutralization by Human IL‑4 Antibody. Recombinant Human IL-4 (Catalog # 204-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-4 Monoclonal Antibody (Catalog # MAB304). The ND50 is typically 0.03-0.1 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-4

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Human IL-4 is synthesized with a 24 aa signal sequence. Alternate splicing generates an isoform with a 16 aa internal deletion. Mature human IL-4 shares 55%, 39%, and 43% aa sequence identity with bovine, mouse, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (5-7). IL-4 exerts its effects through two receptor complexes (8, 9). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma  chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on non-hematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (10-13). IL-4 plays a dominant role in the development of allergic inflammation and asthma (12, 14).

References
  1. Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
  2. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
  3. Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. USA 83:5894.
  4. Redfield, C. et al. (1991) Biochemistry 30:11029.
  5. Ramirez, F. et al. (1988) J. Immunol. Meth. 221:141.
  6. Leitenberg, D. and T.L. Feldbush (1988) Cell. Immunol. 111:451.
  7. Mosman, T.R. et al. (1987) J. Immunol. 138:1813.
  8. Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
  9. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
  10. Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
  11. Corthay, A. (2006) Scand. J. Immunol. 64:93.
  12. Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
  13. Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
  14. Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.
Long Name
Interleukin 4
Entrez Gene IDs
3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit)
Alternate Names
B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; IL4E12; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra

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Citations for Human IL-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Dual vaccination against IL-4 and IL-13 protects against chronic allergic asthma in mice
    Authors: E Conde, R Bertrand, B Balbino, J Bonnefoy, J Stackowicz, N Caillot, F Colaone, S Hamdi, R Houmadi, A Loste, JBJ Kamphuis, F Huetz, L Guillemina, N Gaudenzio, A Mougel, D Hardy, JN Snouwaert, BH Koller, V Serra, P Bruhns, G Grouard-Vo, LL Reber
    Nature Communications, 2021;12(1):2574.
    Species: Mouse
    Sample Types: BALF
    Applications: ELISA Capture
  2. Pilot Study of Anti-Th2 Immunotherapy for the Treatment of Breast Cancer-Related Upper Extremity Lymphedema
    Authors: BJ Mehrara, HJ Park, RP Kataru, J Bromberg, M Coriddi, JE Baik, J Shin, C Li, MR Cavalli, EM Encarnacio, M Lee, KJ Van Zee, E Riedel, JH Dayan
    Biology, 2021;10(9):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. The long intergenic noncoding RNA landscape of human lymphocytes highlights the regulation of T cell differentiation by linc-MAF-4.
    Authors: Ranzani, Valeria, Rossetti, Grazisa, Panzeri, Ilaria, Arrigoni, Alberto, Bonnal, Raoul J, Curti, Serena, Gruarin, Paola, Provasi, Elena, Sugliano, Elisa, Marconi, Maurizio, De Francesco, Raffaele, Geginat, Jens, Bodega, Beatrice, Abrignani, Sergio, Pagani, Massimil
    Nat Immunol, 2015;16(3):318-25.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Human osteoarthritic cartilage shows reduced in vivo expression of IL-4, a chondroprotective cytokine that differentially modulates IL-1beta-stimulated production of chemokines and matrix-degrading enzymes in vitro.
    Authors: Assirelli E, Pulsatelli L, Dolzani P, Platano D, Olivotto E, Filardo G, Trisolino G, Facchini A, Borzi R, Meliconi R
    PLoS ONE, 2014;9(5):e96925.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  5. IL-17 amplifies human contact hypersensitivity by licensing hapten nonspecific Th1 cells to kill autologous keratinocytes.
    Authors: Pennino D, Eyerich K, Scarponi C, Carbone T, Eyerich S, Nasorri F, Garcovich S, Traidl-Hoffmann C, Albanesi C, Cavani A
    J. Immunol., 2010;184(9):4880-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Computed tomographic scan-diagnosed chronic obstructive pulmonary disease-emphysema: eotaxin-1 is associated with bronchodilator response and extent of emphysema.
    Authors: Miller M, Ramsdell J, Friedman PJ, Cho JY, Renvall M, Broide DH
    J. Allergy Clin. Immunol., 2007;120(5):1118-25.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  7. Interleukin-4 induces human hepatocyte apoptosis through a Fas-independent pathway.
    Authors: Aoudjehane L, Podevin P, Scatton O, Jaffray P, Dusanter-Fourt I, Feldmann G, Massault PP, Grira L, Bringuier A, Dousset B, Chouzenoux S, Soubrane O, Calmus Y, Conti F
    FASEB J., 2007;21(7):1433-44.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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Human IL-4 Antibody
By Anonymous on 10/12/2021
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Neurons Species: Human