Human IL-6 R alpha Antibody

(17 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human IL-6 R in ELISAs and Western blots. In ELISAs, no cross-reactivity or interference was observed with recombinant human
    (rh) IL-1 alpha, recombinant mouse (rm) IL-1 alpha, rhIL-1 beta, rmIL-1 beta, rhIL-1ra, rhIL-2, rhIL-3, rmIL-3, rhIL-4, rmIL-4, rhIL-5, rmIL-5, rhIL-6, rmIL-6, rhIL-7, rmIL-7, rhIL-8, rhIL-9, rmIL-9, rhIL-10, or rhIL-11.
  • Source
    Monoclonal Mouse IgG1 Clone # 17506
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑6 R
    Leu20-Asp339
    Accession # P08887
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
    • Human IL-6 R alpha Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human IL‑6 R alpha Antibody (Catalog # MAB227)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human IL‑6 R alpha Biotinylated Antibody (Catalog # BAF227)
  • ELISA Standard
     
    Recombinant Human IL-6 R alpha Protein (Catalog # 227-SR)
  • Neutralization

    Measured by its ability to neutralize IL‑6 R alpha -mediated enhancement of IL‑6-dependent Inhibition of cell proliferation in the M1 mouse myeloid leukemia cell line. The Neutralization Dose (ND50) is typically 0.04-0.2 µg/mL in the presence of 30 ng/mL Recombinant Human IL‑6 R alpha and 30 ng/mL Recombinant Human IL‑6.

     

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Recombinant Human IL‑6 R alpha by Western Blot. Western blot shows 25 ng of Recombinant Human IL‑6 R alpha (Catalog # 227-SR) and Recombinant Mouse IL‑6 R alpha (Catalog # 1830-SR). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human IL‑6 R alpha Monoclonal Antibody (Catalog # MAB227) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL‑6 R alpha at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Detection of IL‑6 R alpha in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was labeled with Human IL‑6 R alpha Monoclonal Antibody (Catalog # MAB227, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG
F(ab')2 Secondary Antibody (Catalog # F0102B).
IL‑6 R alpha Enhancement of
IL‑6-dependent Inhibition of Cell Proliferation and Neutralization by Human
IL‑6 R alpha Antibody.
Recombinant Human IL‑6 R alpha (Catalog #
227-SR) enhances Recombinant Human IL‑6 (Catalog # 206-IL) inhibition of proliferation in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line). Enhancement of Recombinant Human IL‑6 (30 ng/mL) activity elicited by Recombinant Human IL‑6 R alpha (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑6 R alpha Monoclonal Antibody (Catalog # MAB227). The ND50 is typically
0.04-0.2 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-6 R alpha

The multi-functional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6 R alpha ) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6 R alpha and gp130 have been cloned, sequenced, and expressed (1-4).

A soluble form of the IL-6 R alpha has been found in the urine of healthy adult humans (5). This soluble receptor apparently arises from proteolytic cleavage of membrane-bound IL-6 R alpha and is about 50kDa in size. No naturally-occurring mRNA encoding a truncated form of the IL-6 R alpha has been reported. Soluble forms of human and murine IL-6 R alpha s have been constructed, however, by insertion of termination codons into the regions of the IL-6 R alpha cDNAs encoding the external portions of the receptors and prior to the transmembrane domains. These soluble receptors have been expressed in COS-7 and CHO cells and have been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL-6/IL-6 R alpha complex to membrane-bound gp130 (6, 7).

  • References:
    1. Yamasaki et al. (1988) Science 241:825.
    2. Baumann et al. (1990) J. Biol. Chem. 265:19853.
    3. Hibi et al. (1990) Cell 63:1149.
    4. Schooltink et al. (1991) Eur. J. Biochem. 277:659.
    5. Novick et al. (1989) J. Exp. Med. 170:1409.
    6. Yasukawa et al. (1990) J. Biochem. 108:673.
    7. Saito et al. (1991) J. Immunology 147:168.
  • Long Name:
    Interleukin 6 Receptor alpha
  • Entrez Gene IDs:
    3570 (Human); 16194 (Mouse)
  • Alternate Names:
    CD126; IL-6 R alpha; IL6R alpha; IL6R; IL-6Ra
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
Filter your results:

Species
Applications
Sample Type
  1. IL-6 trans-signaling is another pathway to upregulate Osteopontin
    Cytokine, 2016;90(0):88-95.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow Ctyometry
  2. Comparative Ability of Mesenchymal Stromal Cells from Different Tissues to Limit Neutrophil Recruitment to Inflamed Endothelium
    PLoS ONE, 2016;11(5):e0155161.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  3. Contact-dependent depletion of hydrogen peroxide by catalase is a novel mechanism of myeloid-derived suppressor cell induction operating in human hepatic stellate cells.
    Authors: Resheq Y, Li K, Ward S, Wilhelm A, Garg A, Curbishley S, Blahova M, Zimmermann H, Jitschin R, Mougiakakos D, Mackensen A, Weston C, Adams D
    J Immunol, 2015;194(6):2578-86.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  4. Vitamin D regulates cytokine patterns secreted by dendritic cells to promote differentiation of IL-22-producing T cells.
    Authors: Sommer A, Fabri M
    PLoS ONE, 2015;10(6):e0130395.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  5. Mutant B-RAF-Mcl-1 survival signaling depends on the STAT3 transcription factor.
    Authors: Becker, T M, Boyd, S C, Mijatov, B, Gowrishankar, K, Snoyman, S, Pupo, G M, Scolyer, R A, Mann, G J, Kefford, R F, Zhang, X D, Rizos, H
    Oncogene, 2014;33(9):1158-66.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  6. Pathogen-induced human TH17 cells produce IFN-gamma or IL-10 and are regulated by IL-1beta.
    Authors: Zielinski CE, Mele F, Aschenbrenner D, Jarrossay D, Ronchi F, Gattorno M, Monticelli S, Lanzavecchia A, Sallusto F
    Nature, 2012;484(7395):514-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  7. Adipocytes promote ovarian cancer metastasis and provide energy for rapid tumor growth.
    Authors: Nieman KM, Kenny HA, Penicka CV, Ladanyi A, Buell-Gutbrod R, Zillhardt MR, Romero IL, Carey MS, Mills GB, Hotamisligil GS, Yamada SD, Peter ME, Gwin K, Lengyel E
    Nat. Med., 2011;17(11):1498-503.
    Species: Human
    Sample Type: In Vivo
    Application: In Vivo Homing
  8. Interleukin-6 in the bone marrow microenvironment promotes the growth and survival of neuroblastoma cells.
    Authors: Ara T, Song L, Shimada H, Keshelava N, Russell HV, Metelitsa LS, Groshen SG, Seeger RC, DeClerck YA
    Cancer Res., 2009;69(1):329-37.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  9. Production of interleukin 22 but not interleukin 17 by a subset of human skin-homing memory T cells.
    Authors: Duhen T, Geiger R, Jarrossay D, Lanzavecchia A, Sallusto F
    Nat. Immunol., 2009;10(8):857-63.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  10. IL-6 cytoprotection in hyperoxic acute lung injury occurs via suppressor of cytokine signaling-1-induced apoptosis signal-regulating kinase-1 degradation.
    Authors: Kolliputi N, Waxman AB
    Am. J. Respir. Cell Mol. Biol., 2009;40(3):314-24.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  11. Interleukins 1beta and 6 but not transforming growth factor-beta are essential for the differentiation of interleukin 17-producing human T helper cells.
    Authors: Acosta-Rodriguez EV, Napolitani G, Lanzavecchia A, Sallusto F
    Nat. Immunol., 2007;8(9):942-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  12. Effect of IL-6 trans-signaling on the pro-remodeling phenotype of airway smooth muscle.
    Authors: Ammit AJ, Moir LM, Oliver BG, Hughes JM, Alkhouri H, Ge Q, Burgess JK, Black JL, Roth M
    Am. J. Physiol. Lung Cell Mol. Physiol., 2007;292(1):L199-206.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  13. trans fatty acids and systemic inflammation in heart failure.
    Authors: Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC
    Am. J. Clin. Nutr., 2004;80(6):1521-5.
    Species: Human
    Sample Type: Plasma
    Application: ELISA Development
  14. Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells.
    Authors: Hurst SM, McLoughlin RM, Monslow J, Owens S, Morgan L, Fuller GM, Topley N, Jones SA
    J. Immunol., 2002;169(9):5244-51.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  15. Gp130 activation by soluble interleukin-6 receptor/interleukin-6 enhances osteoblastic differentiation of human bone marrow-derived mesenchymal stem cells.
    Authors: Erices A, Conget P, Rojas C, Minguell JJ
    Exp. Cell Res., 2002;280(1):24-32.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  16. Il-6 and its soluble receptor orchestrate a temporal switch in the pattern of leukocyte recruitment seen during acute inflammation.
    Authors: Hurst SM, Wilkinson TS, McLoughlin RM, Jones S, Horiuchi S, Yamamoto N, Rose-John S, Fuller GM, Topley N, Jones SA
    Immunity, 2001;14(6):705-14.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  17. Mechanisms of growth control of Kaposi's sarcoma-associated herpes virus-associated primary effusion lymphoma cells.
    Authors: Asou H, Said JW, Yang R, Munker R, Park DJ, Kamada N, Koeffler HP
    Blood, 1998;91(7):2475-81.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: WB
Expand to show all 17 Citations
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 138  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG1 Isotype Control

Ctrl MAB002R 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG Antibody

WB AF007 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Average Rating: 5 (Based on 1 review)

Have you used Human IL-6 R alpha Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Read what people are saying who have used Human IL-6 R alpha Antibody.

We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Western Blot.

Filter by Ratings
Excellent
(1)
All Ratings
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species

Images Ratings Applications Species Reviewed By Date Details
  Excellent
 WB Human Anonymous 11/29/2017
Show

Summary

ApplicationWestern Blot
Sample TestedStimulated Human Bronchial Epithelial Cell Line (BEAS-2B)
SpeciesHuman

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information