Human IL1RAPL2 Antibody
Human IL1RAPL2 Antibody Summary
Accession # Q9NP60
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
IL1RAPL2 in Human Brain (Cortex). IL1RAPL2 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human IL1RAPL2 Monoclonal Antibody (Catalog # MAB10071) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
The Interleukin 1 receptor family (IL-1 R) comprises at least eleven members including IL-1 RI (IL-1 R1), IL-1 RII (IL-1 R2), IL-1 RAcP (IL-1 R3), ST2 (T1/IL-1 R4), IL‑18 R alpha (IL-1 Rrp/IL-1 R5), IL-1 Rrp2 (IL-1 RL2/IL-1 R6), IL‑18 R beta (AcPL/IL-1 R7), IL-1 RAPL1 (TIGIRR-2/IL-1 R8), and IL-1 RAPL2 (TIGIRR-1/IL-1 R9) (1). All family members possess three immunoglobulin (Ig)-like domains in their extracellular region. Most members also have an intracellular TIR (Toll-like receptor/IL-1 receptor signaling) domain that is also conserved in the Toll-like receptor family. Related proteins, SIGIRR (single Ig domain-containing IL-1 R-related molecule) and IL‑18BP, differ from the other members by having only one Ig domain (1). IL-1 receptor accessory protein-like 2 (IL-1 RAPL2) is alternately known as IL-1 R9 and three immunoglobulin domain containing IL-1 receptor-related molecule 1 (TIGIRR-1) and is expressed in the brain (2). Its sequence predicts an 686 amino acid (aa) residue type I transmembrane glycoprotein with a 17 aa signal peptide, a 339 aa extracellular region containing three Ig-like domains, an 18 aa transmembrane domain and a 312 aa cytoplasmic tail (3). By comparison to other IL-1 receptor family proteins, IL-1 RAPL2 has a C-terminal cytoplasmic extension beyond the TIR domain that is found in IL-1 RAPL1 and SIGIRR but not other family members (3). Human and mouse IL‑1 RAPL2 share approximately 95% aa sequence identity. Human IL‑1 RAPL2 is most homologous (63%) to IL‑1 RAPL1, a receptor protein that is highly expressed in hippocampus and is involved in X-linked mental retardation (4, 5). Genes for both have been localized to human chromosome Xq22. A ligand for IL-1 RAPL2 has not been identified (1).
- Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
- Andre, R. et al. (2005) J. Neurochem. 95:324.
- Born, T.L. et al. (2000) J. Biol. Chem. 275:29946.
- Jin, H. et al. (2000) Eur. J. Hum. Genet. 8:87.
- Carrie, A. et al. (1999) Nat. Genet. 23:25.
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