The Interleukin 1 receptor family (IL-1 R) comprises at least eleven members including IL-1 RI (IL-1 R1), IL-1 RII (IL-1 R2), IL-1 RAcP (IL-1 R3), ST2 (T1/IL-1 R4), IL‑18 R alpha (IL-1 Rrp/IL-1 R5), IL-1 Rrp2 (IL-1 RL2/IL-1 R6), IL‑18 R beta (AcPL/IL-1 R7), IL-1 RAPL1 (TIGIRR-2/IL-1 R8), and IL-1 RAPL2 (TIGIRR-1/IL-1 R9) (1). All family members possess three immunoglobulin (Ig)-like domains in their extracellular region. Most members also have an intracellular TIR (Toll-like receptor/IL-1 receptor signaling) domain that is also conserved in the Toll-like receptor family. Related proteins, SIGIRR (single Ig domain-containing IL-1 R-related molecule) and IL‑18BP, differ from the other members by having only one Ig domain (1). IL-1 receptor accessory protein-like 2 (IL-1 RAPL2) is alternately known as IL-1 R9 and three immunoglobulin domain containing IL-1 receptor-related molecule 1 (TIGIRR-1) and is expressed in the brain (2). Its sequence predicts an 686 amino acid (aa) residue type I transmembrane glycoprotein with a 17 aa signal peptide, a 339 aa extracellular region containing three Ig-like domains, an 18 aa transmembrane domain and a 312 aa cytoplasmic tail (3). By comparison to other IL-1 receptor family proteins, IL-1 RAPL2 has a C-terminal cytoplasmic extension beyond the TIR domain that is found in IL-1 RAPL1 and SIGIRR but not other family members (3). Human and mouse IL‑1 RAPL2 share approximately 95% aa sequence identity. Human IL‑1 RAPL2 is most homologous (63%) to IL‑1 RAPL1, a receptor protein that is highly expressed in hippocampus and is involved in X-linked mental retardation (4, 5). Genes for both have been localized to human chromosome Xq22. A ligand for IL-1 RAPL2 has not been identified (1).
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 167720
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL‑1 RAPL2
Thr17-Glu356
Accession # Q9NP60
Thr17-Glu356
Accession # Q9NP60
Specificity
Detects human IL-1 RAPL2 in ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human IL1RAPL2 Antibody (167720)
IL1RAPL2 in Human Brain (Cortex).
IL1RAPL2 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human IL1RAPL2 Monoclonal Antibody (Catalog # MAB10071) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human IL1RAPL2 Antibody (167720)
Application
Recommended Usage
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: IL1RAPL2
References
- Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
- Andre, R. et al. (2005) J. Neurochem. 95:324.
- Born, T.L. et al. (2000) J. Biol. Chem. 275:29946.
- Jin, H. et al. (2000) Eur. J. Hum. Genet. 8:87.
- Carrie, A. et al. (1999) Nat. Genet. 23:25.
Long Name
Interleukin 1 Receptor Accessory Protein-like 2
Alternate Names
IL-1R10, IL-1RAPL2, IL1RAPL2, TIGIRR-1
Gene Symbol
IL1RAPL2
UniProt
Additional IL1RAPL2 Products
Product Documents for Human IL1RAPL2 Antibody (167720)
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human IL1RAPL2 Antibody (167720)
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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