Human IRF8 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human IRF8 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human IRF4 is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 809926
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human IRF8
    Ala255-Val426
    Accession # Q02556
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Simple Western
    20 µg/mL
    See below
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of Human IRF8 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line. Gels were loaded with 25 µg of nuclear extracts (Nuc) and whole cell lysate (WCL). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IRF8 Monoclonal Antibody (Catalog # MAB5117) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IRF8 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

IRF8 in Human Tonsil. IRF8 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human IRF8 Monoclonal Antibody (Catalog # MAB5117) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes in germinal centers. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human IRF8 by Simple WesternTM. Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line, loaded at 0.5 mg/mL. A specific band was detected for IRF8 at approximately 54 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human IRF8 Monoclonal Antibody (Catalog # MAB5117). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IRF8
IRF8 (interferon regulatory factor 8; also ICSBP) is a 50 kDa member of the IRF family of proteins. It is expressed in myeloid, dendritic and B cells and interacts with PU.1 to regulate the expression of TLR4, IL-18, IL-12p35, and PTPN13, a Fas-associated phosphatase that blocks Fas-induced apoptosis. Human IRF8 is 426 amino acids (aa) in length. It contains an N-terminal DNA-binding basic region (aa 9-110) and at least one regulatory phosphorylation site at Tyr95. Over aa 255-426, human IRF8 shares 90% and 88% aa sequence identity with canine and mouse IRF8, respectively.
  • Long Name:
    Interferon Regulatory Factor 8
  • Entrez Gene IDs:
    3394 (Human); 15900 (Mouse); 292060 (Rat)
  • Alternate Names:
    ICSBP1; ICSBPH-ICSBP; interferon consensus sequence binding protein 1; Interferon consensus sequence-binding protein; interferon regulatory factor 8; IRF8; IRF-8ICSBP1
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