Human Irisin/FNDC5 Antibody

Catalog # Availability Size / Price Qty
MAB9420-100
MAB9420-SP
Detection of Irisin/FNDC5 in HepG2 Human Cell Line by Flow Cytometry.
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Product Details
Citations (1)
FAQs
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Reviews (1)

Human Irisin/FNDC5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Irisin/FNDC5 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 936434
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Irisin/FNDC5
Asp32-Glu143
Accession # Q8NAU1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Irisin/FNDC5 Monoclonal Antibody (Catalog # MAB8880).

This product is intended for assay development on various assay platforms requiring antibody pairs.

 
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
4-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Irisin/FNDC5 antibody in HepG2 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Irisin/FNDC5 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocarcinoma cell line, cultured in media containing low glucose and 100 nM insulin, was stained with Mouse Anti-Human Irisin/FNDC5 Monoclonal Antibody (Catalog # MAB9420, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by APC-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry Irisin/FNDC5 antibody in Rat Skeletal Muscle by Immunohistochemistry (IHC-Fr). View Larger

Irisin/FNDC5 in Rat Skeletal Muscle. Irisin/FNDC5 was detected in perfusion fixed frozen sections of rat skeletal muscle using Mouse Anti-Human Irisin/FNDC5 Monoclonal Antibody (Catalog # MAB9420) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm of muscle cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Irisin/FNDC5

Irisin (also known as FNDC5) is a 12 kDa glycosylated polypeptide hormone that regulates energy metabolism, stem cell differentiation, and neuronal development (1, 2). Human Irisin is synthesized as a 212 amino acid (aa) precursor encoding a type 1 transmembrane protein with a 121 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 39 aa cytoplasmic domain. The ECD of Irisin contains a fibronectin type III domain and multiple glycosylation sites. The ECD is proteolytically cleaved to release the 112 aa soluble Irisin hormone into circulation (2-5). Mature human, mouse, and rat Irisin share 100% sequence identity. Expression of Irisin is induced in skeletal muscle and subcutaneous adipose tissue during and shortly after exercise (2, 6). Irisin induces expression of peroxisome proliferator-activated receptor  gamma co-activator 1 alpha (PGC1 alpha ) and uncoupling protein-1 (UCP1), mitochondrial-associated metabolic proteins (7, 8). Irisin induces the transition of white adipose tissue into more metabolically active beige adipose tissue. In mice, expression of Irisin has been shown to regulate obesity and diabetes (1, 2). A similar function in humans is suggested (9). Irisin also regulates neuronal cell differentiation and neurite outgrowth in the brain and is involved in the differentiation of osteoblasts (10-14).

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Citation for Human Irisin/FNDC5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Plasma-derived extracellular vesicles released after endurance exercise exert cardioprotective activity through the activation of antioxidant pathways
    Authors: Lisi, V;Senesi, G;Bertola, N;Pecoraro, M;Bolis, S;Gualerzi, A;Picciolini, S;Raimondi, A;Fantini, C;Moretti, E;Parisi, A;Sgr�, P;Di Luigi, L;Geiger, R;Ravera, S;Vassalli, G;Caporossi, D;Balbi, C;
    Redox biology
    Species: Human
    Sample Types: Plasma
    Applications: Surface Plasmon Resonance (SPR)

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Human Irisin/FNDC5 Antibody
By Anonymous on 06/28/2022
Application: IHC Sample Tested: Skeletal muscle tissue Species: Human