Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, ELISA, Flow Cytometry, CyTOF-ready

Cited:

Surface Plasmon Resonance (SPR)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 936434
View all Irisin/FNDC5 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Irisin/FNDC5
Asp32-Glu143
Accession # Q8NAU1

Specificity

Detects human Irisin/FNDC5 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Irisin/FNDC5 Antibody

Detection of Irisin/FNDC5 antibody in HepG2 Human Cell Line antibody by Flow Cytometry.

Detection of Irisin/FNDC5 in HepG2 Human Cell Line by Flow Cytometry.

HepG2 human hepatocarcinoma cell line was stained with Mouse Anti-Human Irisin/FNDC5 Monoclonal Antibody (Catalog # MAB9420, filled histogram) or isotype control antibody (MAB0041, open histogram), followed by APC-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (F0101B). View our protocol for Staining Membrane-associated Proteins.
Irisin/FNDC5 antibody in Rat Skeletal Muscle by Immunohistochemistry (IHC-Fr).

Irisin/FNDC5 in Rat Skeletal Muscle.

Irisin/FNDC5 was detected in perfusion fixed frozen sections of rat skeletal muscle using Mouse Anti-Human Irisin/FNDC5 Monoclonal Antibody (Catalog # MAB9420) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm of muscle cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Immersion fixed paraffin-embedded sections of human skeletal muscle

Irisin/FNDC5 was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Mouse Anti-Human Irisin/FNDC5 Monoclonal Antibody (Catalog # MAB9420) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Irisin/FNDC5 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Irisin/FNDC5 Monoclonal Antibody (Catalog # MAB8880).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Flow Cytometry

0.25 µg/106 cells
Sample: HepG2 cell line

Immunohistochemistry

0.25-25 µg/mL
Sample:

Perfusion fixed frozen sections of rat skeletal muscle and Immersion fixed paraffin-embedded sections of human skeletal muscle

Reviewed Applications

Read 1 review rated 5 using MAB9420 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Irisin/FNDC5

Irisin (also known as FNDC5) is a 12 kDa glycosylated polypeptide hormone that regulates energy metabolism, stem cell differentiation, and neuronal development (1, 2). Human Irisin is synthesized as a 212 amino acid (aa) precursor encoding a type 1 transmembrane protein with a 121 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 39 aa cytoplasmic domain. The ECD of Irisin contains a fibronectin type III domain and multiple glycosylation sites. The ECD is proteolytically cleaved to release the 112 aa soluble Irisin hormone into circulation (2-5). Mature human, mouse, and rat Irisin share 100% sequence identity. Expression of Irisin is induced in skeletal muscle and subcutaneous adipose tissue during and shortly after exercise (2, 6). Irisin induces expression of peroxisome proliferator-activated receptor  gamma co-activator 1 alpha (PGC1 alpha ) and uncoupling protein-1 (UCP1), mitochondrial-associated metabolic proteins (7, 8). Irisin induces the transition of white adipose tissue into more metabolically active beige adipose tissue. In mice, expression of Irisin has been shown to regulate obesity and diabetes (1, 2). A similar function in humans is suggested (9). Irisin also regulates neuronal cell differentiation and neurite outgrowth in the brain and is involved in the differentiation of osteoblasts (10-14).

References

  1. Bostrom, P. et al. (2012) Nature 481:463.
  2. Novelle, M.G. et al. (2013) Int. J. Endocrinol. 2013:746281.
  3. Ferrer-Martinez, A. et al. (2002) Dev. Dyn. 224:154.
  4. Teufel, A. et al. (2002) Gene 297:79.
  5. Zhang, W. et al. (2015) Neurosci. Lett. 595:7.
  6. Roca-Rivada, A. et al. (2013) PLoS One 8:e60563.
  7. Vaughan, R.A. et al. (2014) Diabetes Obes. Metab. 16:711.
  8. Jeong Lee, H. et al. (2015) Mol. Endocrinol. 6:873.
  9. Elsen, M. et al. (2014) J. Endocrinol. 222:R25.
  10. Dun, S.L. et al. (2013) Neuroscience 240:155.
  11. Forouzanfar, M. et al. (2015) Cell. Biol. Int. 39:629.
  12. Hashemi, M.S. et al. (2013) Neuroscience 231:296.
  13. Moon, H.S. et al. (2013) Metabolism 62:1131.
  14. Colaianni, G. et al. (2014) Int. J. Endocrinol. 2014:902186.

Long Name

Fibronectin Type III Domain Containing 5

Alternate Names

FNDC5, FRCP2

Entrez Gene IDs

252995 (Human); 384061 (Mouse); 260327 (Rat)

Gene Symbol

FNDC5

UniProt

Q8NAU1

Additional Irisin/FNDC5 Products

Product Documents for Human Irisin/FNDC5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Irisin/FNDC5 Antibody

For research use only

Related Research Areas

Diabetes
Obesity

Citations for Human Irisin/FNDC5 Antibody

Customer Reviews for Human Irisin/FNDC5 Antibody (1)

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  • Human Irisin/FNDC5 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Skeletal muscle tissue
    Species: Human
    Verified Customer | Posted 06/28/2022
    Human Irisin/FNDC5 Antibody MAB9420

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Protocols

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