Detection of Human ISG15/UCRP by Western Blot.
Western blot shows lysates of MCF‑7 human breast cancer cell line and HT‑29 human colon adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human ISG15/UCRP Monoclonal Antibody (Catalog # MAB4845) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ISG15/UCRP at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
ISG15 (interferon-induced 15 kDa protein; also known as IP17) is a 15 kDa ubiquitin family protein that is expressed by a wide variety of cell types. ISG15 is induced by type I IFN, and like ubiquitin, becomes conjugated to intracellular proteins. Unlike ubiquitin, it does not target proteins for degradation. Instead, it is part of the antivirus response. Human proISG15 is 165 amino acids (aa) in length. There are two ubiquitin-like domains (aa 2‑78 and 79‑157) and a propeptide (aa 158‑165) that is cleaved intracellularly. Select blood mononuclear cells secrete mature ISG15, which is chemotactic for neutrophils. Mature human ISG15 shares 66% aa sequence identity with mouse ISG15.
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