Human ISG15/UCRP Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human ISG15/UCRP in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG2B Clone # 539442
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human ISG15/UCRP
    Gly2-Gly157
    Accession # ABW03539
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Immunohistochemistry
    8-25 µg/mL
    Immersion fixed paraffin-embedded sections of human endometrial cancer tissue
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human ISG15/UCRP by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line and HT‑29 human colon adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human ISG15/UCRP Monoclonal Antibody (Catalog # MAB4845) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ISG15/UCRP at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ISG15/UCRP
ISG15 (interferon-induced 15 kDa protein; also known as IP17) is a 15 kDa ubiquitin family protein that is expressed by a wide variety of cell types. ISG15 is induced by type I IFN, and like ubiquitin, becomes conjugated to intracellular proteins. Unlike ubiquitin, it does not target proteins for degradation. Instead, it is part of the antivirus response. Human proISG15 is 165 amino acids (aa) in length. There are two ubiquitin-like domains (aa 2‑78 and 79‑157) and a propeptide (aa 158‑165) that is cleaved intracellularly. Select blood mononuclear cells secrete mature ISG15, which is chemotactic for neutrophils. Mature human ISG15 shares 66% aa sequence identity with mouse ISG15.
  • Long Name:
    ISG15 Ubiquitin-like Modifier
  • Entrez Gene IDs:
    9636 (Human); 100038882 (Mouse); 298693 (Rat)
  • Alternate Names:
    G1P2; G1P2interferon-induced 17-kDa/15-kDa protein; IFI15; IFI15hUCRP; interferon, alpha-inducible protein (clone IFI-15K); Interferon-induced 15 kDa protein; Interferon-induced 17 kDa protein; interferon-stimulated protein, 15 kDa; IP17; ISG15 ubiquitin-like modifier; ISG15; Ubiquitin cross-reactive protein; ubiquitin-like protein ISG15; UCRP; UCRPIP17
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Vitamin D receptor and Jak-STAT signaling crosstalk results in calcitriol-mediated increase of hepatocellular response to IFN-alpha.
    Authors: Lange C, Gouttenoire J, Duong F, Morikawa K, Heim M, Moradpour D
    J Immunol, 2014;192(12):6037-44.
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