Human ITIH2 Antibody

Catalog # Availability Size / Price Qty
MAB101991-100
MAB101991-SP
Detection of Human ITIH2 by Western Blot.
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Human ITIH2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ITIH2 in direct ELISA.
Source
Monoclonal Mouse IgG2B Clone # 1023658
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Spodoptera frugiperda Sf 9 (baculovirus)-derived human ITIH2
Ser55-Asp702
Accession # P19823
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
HepG2 human hepatocellular carcinoma cell line
ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human ITIH2 Monoclonal Antibody (Catalog # MAB101993).

This product is intended for assay development on various assay platforms requiring antibody pairs.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot View Larger

Detection of Human ITIH2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ITIH2 Monoclonal Antibody (Catalog # MAB101991) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for ITIH2 at approximately 106 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Human ITIH2 ELISA Standard Curve. Recombinant Human ITIH2 protein was serially diluted 2-fold and captured by Mouse Anti-Human ITIH2 Monoclonal Antibody (MAB101993) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human ITIH2 Monoclonal Antibody (Catalog # MAB101991) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ITIH2

The ITIs are a family of plasma protease inhibitors contributing to extracellular matrix stabilization by covalent linkage to hyaluronan.  ITIH2 is a binding protein between hyaluronan and other matrix proteins.  It regulates the localization, synthesis and degradation of hyaluronan.  ITIH molecules are shown to play a role in carcinogenesis and inflammation.  ITIH genes are downregulated in multiple human solid tumors.  This downregulation contributes to initiation and/or progression of malignancy. 

Long Name
Inter-Alpha-Trypsin Inhibitor Heavy Chain 2
Entrez Gene IDs
3698 (Human); 16425 (Mouse)
Alternate Names
H2P; IGHEP2; Inter-Alpha (Globulin) Inhibitor H2; inter-alpha (globulin) inhibitor, H2 polypeptide; Inter-alpha-inhibitor heavy chain 2; Inter-alpha-trypsin inhibitor complex component II; inter-alpha-trypsin inhibitor heavy chain H2; Intin2; ITI Heavy Chain H2; ITIH2; ITI-H2; ITI-HC2; Serum-derived hyaluronan-associated protein; SHAP

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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