Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1023658
View all ITIH2 Primary Antibodies »

Product Specifications

Immunogen

Spodoptera frugiperda Sf 9 (baculovirus)-derived human ITIH2
Ser55-Asp702
Accession # P19823

Specificity

Detects human ITIH2 in direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human ITIH2 Antibody

Detection of Human ITIH2 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ITIH2 Monoclonal Antibody (Catalog # MAB101991) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for ITIH2 at approximately 106 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Human ITIH2 ELISA Standard Curve.

Recombinant Human ITIH2 protein was serially diluted 2-fold and captured by Mouse Anti-Human ITIH2 Monoclonal Antibody (MAB101993) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human ITIH2 Monoclonal Antibody (Catalog # MAB101991) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Applications for Human ITIH2 Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human ITIH2 Monoclonal Antibody (Catalog # MAB101993).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Western Blot

2 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ITIH2

The ITIs are a family of plasma protease inhibitors contributing to extracellular matrix stabilization by covalent linkage to hyaluronan.  ITIH2 is a binding protein between hyaluronan and other matrix proteins.  It regulates the localization, synthesis and degradation of hyaluronan.  ITIH molecules are shown to play a role in carcinogenesis and inflammation.  ITIH genes are downregulated in multiple human solid tumors.  This downregulation contributes to initiation and/or progression of malignancy. 

Long Name

Inter-Alpha-Trypsin Inhibitor Heavy Chain 2

Alternate Names

H2P, IGHEP2, Inter-Alpha (Globulin) Inhibitor H2, Intin2, ITI Heavy Chain H2, ITI-H2, ITI-HC2, SHAP

Entrez Gene IDs

3698 (Human); 16425 (Mouse)

Gene Symbol

ITIH2

UniProt

P19823

Additional ITIH2 Products

Product Documents for Human ITIH2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human ITIH2 Antibody

For research use only

Related Research Areas

Serine Proteases and Regulators

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