Human Jagged 1 Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Jagged 1 in direct ELISAs. In direct ELISAs, approximately 50% cross‑reactivity with recombinant human Jagged 2 and recombinant rat Jagged 1 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 188323
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0-derived recombinant human Jagged 1
    Ser32-Asp296
    Accession # P78504
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Neutralization
    Measured by its ability to neutralize Jagged 1-induced alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line. The Neutralization Dose (ND50) is typically 0.5-3 µg/mL in the presence of 5 µg/mL Recombinant Human Jagged 1 Fc Chimera.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Alkaline Phosphatase Production Induced by Jagged 1 and Neutralization by Human Jagged 1 Antibody. Recombinant Human Jagged 1 Fc Chimera (Catalog # 1277-JG) induces alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line in the presence of Recombinant Human/Mouse/Rat BMP-2 (Catalog # 355-BM) in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human Jagged 1 Fc Chimera (5 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Jagged 1 Monoclonal Antibody (Catalog # MAB12771). The ND50 is typically 0.5-3 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Jagged 1

Jagged 1 is a 180 kDa type I transmembrane glycoprotein and member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins. Human Jagged 1 is synthesized as a 1218 amino acid (aa) precursor that contains a 33 aa signal sequence, a 1034 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a 125 aa cytoplasmic region. The ECD contains a DSL domain (aa 185‑229), a cysteine-rich region, 15 EGF-like repeats, of which many bind calcium, and nine potential sites for N-linked glycosylation. Mature human Jagged 1 shares 97% and 96% aa identity with mature mouse and rat Jagged 1, respectively. Jagged 1 is widely expressed in adult and fetal tissues. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation, especially during hematopoiesis, myogenesis, neurogenesis, and development of vasculature (1‑8). Mutations in human Jagged 1 are the cause of Alagille syndrome, an autosomal-dominant disorder characterized by intrahepatic cholestasis and abnormalities of heart, eye, vertebrae, as well as characteristic facial appearance (9, 10).

  • References:
    1. Sainson, R.C. and A.L. Harris (2008) Angiogenesis 11:41.
    2. Cordle, J. et al. (2008) Nat. Struct. Mol. Biol. 15:849.
    3. Artavamis-Tsokanas, S. et al. (1999) Science 284:770.
    4. Lai, E.C. (2004) Development 131:965.
    5. Milner, L.A. et al. (1994) Blood 83:2057.  
    6. Nyfeler, Y. et al. (2005) EMBO J. 24:3504.
    7. Linheng, L. et al. (1998) Immunity 8:43.
    8. Iso, T. et al. (2003) Arterioscler. Thromb. Vasc. Biol. 23:543.
    9. Oda, T. et al. (1997) Nat. Genet. 16:235.
    10. Oda, T. et al. (1997) Genomics 43:376.
  • Entrez Gene IDs:
    182 (Human); 29146 (Rat)
  • Alternate Names:
    AGS; AHDMGC104644; Alagille syndrome; AWS; CD339 antigen; CD339; HJ1; JAG1; Jagged 1; Jagged1; JAGL1; protein jagged-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Annexin II expressed by osteoblasts and endothelial cells regulates stem cell adhesion, homing, and engraftment following transplantation.
    Authors: Jung Y, Wang J, Song J, Shiozawa Y, Wang J, Havens A, Wang Z, Sun YX, Emerson SG, Krebsbach PH, Taichman RS
    Blood, 2007;110(0):82.
    Species: Human
    Sample Type: Whole Cells
    Application: Binding Assay
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Isotype Control

Ctrl MAB004 32  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG2B Isotype Control

Ctrl MAB0041 7  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG2B Isotype Control

Ctrl MAB0042 6
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG Antibody

WB AF007 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133 1  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

Flow F0134 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat Anti-Mouse IgG2B PE-conjugated Antibody

Flow F0132  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG2B Antibody

Flow MAB0043  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human Jagged 1 Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Associated Pathways

Feedback

Customer Information