Human Jagged 1 Antibody
Human Jagged 1 Antibody Summary
Accession # P78504
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Alkaline Phosphatase Production Induced by Jagged 1 and Neutralization by Human Jagged 1 Antibody. Recombinant Human Jagged 1 Fc Chimera (Catalog # 1277-JG) induces alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line in the presence of Recombinant Human/Mouse/Rat BMP-2 (Catalog # 355-BM) in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human Jagged 1 Fc Chimera (5 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Jagged 1 Monoclonal Antibody (Catalog # MAB12771). The ND50 is typically 0.5-3 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Jagged 1
Jagged 1 is a 180 kDa type I transmembrane glycoprotein and member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins. Human Jagged 1 is synthesized as a 1218 amino acid (aa) precursor that contains a 33 aa signal sequence, a 1034 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a 125 aa cytoplasmic region. The ECD contains a DSL domain (aa 185‑229), a cysteine-rich region, 15 EGF-like repeats, of which many bind calcium, and nine potential sites for N-linked glycosylation. Mature human Jagged 1 shares 97% and 96% aa identity with mature mouse and rat Jagged 1, respectively. Jagged 1 is widely expressed in adult and fetal tissues. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation, especially during hematopoiesis, myogenesis, neurogenesis, and development of vasculature (1‑8). Mutations in human Jagged 1 are the cause of Alagille syndrome, an autosomal-dominant disorder characterized by intrahepatic cholestasis and abnormalities of heart, eye, vertebrae, as well as characteristic facial appearance (9, 10).
- Sainson, R.C. and A.L. Harris (2008) Angiogenesis 11:41.
- Cordle, J. et al. (2008) Nat. Struct. Mol. Biol. 15:849.
- Artavamis-Tsokanas, S. et al. (1999) Science 284:770.
- Lai, E.C. (2004) Development 131:965.
- Milner, L.A. et al. (1994) Blood 83:2057.
- Nyfeler, Y. et al. (2005) EMBO J. 24:3504.
Linheng, L. et al. (1998) Immunity 8:43.
Iso, T. et al. (2003) Arterioscler. Thromb. Vasc. Biol. 23:543.
Oda, T. et al. (1997) Nat. Genet. 16:235.
Oda, T. et al. (1997) Genomics 43:376.
Citations for Human Jagged 1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
Filter your results:
The role of Jagged1 as a dynamic switch of cancer cell plasticity in PDAC assembloids
Authors: JI Choi, JH Rim, SI Jang, JS Park, H Park, JH Cho, JB Lim
Sample Types: Organoid
Annexin II expressed by osteoblasts and endothelial cells regulates stem cell adhesion, homing, and engraftment following transplantation.
Authors: Jung Y, Wang J, Song J, Shiozawa Y, Wang J, Havens A, Wang Z, Sun YX, Emerson SG, Krebsbach PH, Taichman RS
Sample Types: Whole Cells
Applications: Binding Assay
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human Jagged 1 Antibody
There are currently no reviews for this product. Be the first to review Human Jagged 1 Antibody and earn rewards!
Have you used Human Jagged 1 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image