Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Knockout Validated, Multiplex Immunofluorescence, Immunohistochemistry, Intracellular Staining by Flow Cytometry, Dual RNAscope ISH-IHC Compatible, Immunocytochemistry, Simple Western

Cited:

Immunohistochemistry, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1297A
View all Ki67/MKI67 Primary Antibodies »

Product Specifications

Immunogen

Mouse Ki67/MKI67 synthetic peptide Accession #E9PVX6
Accession # E9PVX6

Specificity

Detects human Ki67/MKI67 in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Mouse Ki67/MKI67 Antibody

Detection of Ki67/MKI67 in Human Tonsil via seqIF™ staining on COMET™

Ki67/MKI67 was detected in immersion fixed paraffin-embedded sections of human tonsil using Rabbit Anti-Human Ki67/MKI67 Monoclonal Antibody (Catalog # MAB7617) at 10 µg/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100).. Specific staining was localized to the nucleus. Protocol available in COMET™ Panel Builder.

Detection of Ki67/MKI67 in Human Colon via seqIF™ staining on COMET™

Ki67/MKI67 was detected in immersion fixed paraffin-embedded sections of human Colon using Rabbit Anti-Human Ki67/MKI67 Monoclonal Antibody (Catalog # MAB7617) at 5 µg/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100).. Specific staining was localized to the nucleus. Protocol available in COMET™ Panel Builder.

Detection of Ki67/MKI67 in Human Hodgkin Lymphoma via seqIF™ staining on COMET™

Ki67/MKI67 was detected in immersion fixed paraffin-embedded sections of human Hodgkin Lymphoma using Rabbit Anti-Human Ki67/MKI67 Monoclonal Antibody (Catalog # MAB7617) at 5 µg/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100).. Specific staining was localized to the nucleus. Protocol available in COMET™ Panel Builder.

Detection of Ki67/MKI67 in Human Appendix via seqIF™ staining on COMET™

Ki67/MKI67 was detected in immersion fixed paraffin-embedded sections of human Appendix using Rabbit Anti-Human Ki67/MKI67 Monoclonal Antibody (Catalog # MAB7617) at 5 µg/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100).. Specific staining was localized to the nucleus. Protocol available in COMET™ Panel Builder.

Detection of Ki67 in Mouse Stomach via seqIF™ staining on COMET™​

Ki67 was detected in immersion fixed paraffin-embedded sections of mouse Stomach using Rabbit Anti-Mouse Ki67, Monoclonal Antibody (Catalog # MAB7617) at 0.15ug/mL at 37° Celsius for 2 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the nucleus. Protocol available in COMET™ Panel Builder.
Ki67/MKI67 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

Ki67/MKI67 in HeLa Human Cell Line.

Ki67/MKI67 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human Ki67/MKI67 Monoclonal Antibody (Catalog # MAB7617) at 0.3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Ki67/MKI67 antibody in Human Pancreatic Cancer Tissue by Immunohistochemistry (IHC-P).

Ki67/MKI67 in Human Pancreatic Cancer Tissue.

Ki67/MKI67 was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Rabbit Anti-Human Ki67/MKI67 Monoclonal Antibody (Catalog # MAB7617) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Ki67/MKI67 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of Ki67/MKI67 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) either A) untreated or (B) treated with 5 µg/mL PHA for 5 days were stained with Rabbit Anti-Human Ki67/MKI67 Monoclonal Antibody (Catalog # MAB7617) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (F0110) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (FAB100A). Quadrant markers were set based on control antibody staining (MAB1050). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (FC012). View our protocol for Staining Intracellular Molecules.

Detection of Human Ki67/MKI67 by Simple WesternTM.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and MCF‑7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Ki67/MKI67 at approximately 320 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human Ki67/MKI67 Monoclonal Antibody (Catalog # MAB7617). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.
Ki67/MKI67 Antibody Specificity is Shown by Immunocytochemistry antibody in Knockout Cell Line.

Ki67/MKI67 Specificity is Shown by Immunocytochemistry in Knockout Cell Line.

Ki67/MKI67 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line but is not detected in Ki67/MKI67 knockout (KO) HeLa cell line using Rabbit Anti-Human Ki67/MKI67 Monoclonal Antibody (Catalog # MAB7617) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Human Ki67/MKI67 Specificity Shown by Simple WesternTM in Knockout Cell Line.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and Ki67 knockout HeLa cell line (KO), loaded at 0.2 mg/mL. A specific band was detected for Ki67/MKI67 at approximately 320 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human Ki67/MKI67 Monoclonal Antibody (Catalog # MAB7617). GAPDH (MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.

Ki-67/MKI67 in human breast cancer using Dual RNAscope®ISH and IHC.

MKi67 mRNA (red) and MKi67 protein (green) were detected in formalin-fixed paraffin-embedded tissue sections of human breast cancer. ACD’s Integrated Co-Detection Workflow was performed using ACD RNAScope Probe Hs-MKI67 (Catalog # 591771) and rabbit anti-human Ki67/MKI67 recombinant monoclonal antibody (Catalog # MAB7616) at 10 μg/mL. Tissue was stained using RNAscope® 2.5 HD Detection Kit-RED (Catalog # 322360) and RNAscope® 2.5 LS Green Accessory Pack (Catalog # 322550). Tissue was counterstained with 50% hematoxylin (blue).

Detection of Ki67/MKI67 in Mouse Thymus.

Ki67/MKI67 was detected in immersion fixed paraffin-embedded sections of mouse thymus using Rabbit Anti-Human Ki67/MKI67 Monoclonal Antibody (Catalog # MAB7617) at 0.5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human/Mouse Ki67/MKI67 Antibody

Application
Recommended Usage

Dual RNAscope ISH-IHC Compatible

5-25 µg/mL
Sample: Formalin-fixed paraffin-embedded tissue sections of human breast cancer

Immunocytochemistry

0.3-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreatic cancer tissue and mouse thymus

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with PHA were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)

Knockout Validated

Ki67/MKI67 is specifically detected in Hela human cervical epithelial carcinoma parental cell line but is not detectable in Ki67/MKI67 knockout HeLa cell line.

Multiplex Immunofluorescence

0.15 - 10 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human tonsil and mouse intestine

Simple Western

20 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and MCF‑7 human breast cancer cell line

Reviewed Applications

Read 3 reviews rated 4.3 using MAB7617 in the following applications:

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ki67/MKI67

MKI67 (also Ki67) is a 350-400 kDa nuclear protein that belongs to a molecular group comprised of mitotic chromosome-associated proteins. Ki67 was originally recognized as an antigen associated with the monoclonal Ki67 antibody raised against Hodgkin's lymphoma nuclear material. Ki-67 is contextually expressed, being potentially found in all cells that are not in the Go phase of the cell cycle. Thus, MKI67 qualifies as a cell proliferation marker. Functionally, Ki67 is known to interact with 160 kDa Hklp2, a protein that promotes centrosome separation and spindle bipolarity. It also directly interacts with NIFK, and apparently binds to UBF, thus playing a role in rRNA synthesis. Human MKI67 is 3256 amino acids (aa) in length. It contains one FHA domain (aa 8-98), followed by at least 24 utilized Ser/Thr phosphorylation sites and sixteen 120 aa repeats (aa 1000-2928) that are interspersed with at least 90 additional utilized phosphorylation sites. There are two potential isoform variants. One isoform is 315-345 kDa in size and shows a deletion of aa 136-495, while a second isoform contains a 58 aa substitution for aa 1-513. Over aa 3120-3256, human Ki67 shares 46% aa sequence identity with the mouse ortholog to Ki67.

Long Name

Antigen Identified by Monoclonal Antibody Ki67

Alternate Names

Ki-67, KIA, MIB-1, MKI67, PPP1R105, TSG126

Entrez Gene IDs

4288 (Human); 17345 (Mouse); 291234 (Rat)

Gene Symbol

MKI67

UniProt

E9PVX6

Additional Ki67/MKI67 Products

Product Documents for Human/Mouse Ki67/MKI67 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse Ki67/MKI67 Antibody

For research use only

Related Research Areas

Cancer Biomarkers
Cell Cycle

Citations for Human/Mouse Ki67/MKI67 Antibody

Customer Reviews for Human/Mouse Ki67/MKI67 Antibody (3)

4.3 out of 5
3 Customer Ratings
5 Stars
33%
4 Stars
67%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Human Ki67/MKI67 Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Blood mononuclear cells (PBMCs)
    Species: Human
    Verified Customer | Posted 03/23/2025
    Human/Mouse Ki67/MKI67 Antibody MAB7617
  • Human Ki67/MKI67 Antibody
    Name: Agnès Tessier
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Skin tissue
    Species: Human
    Verified Customer | Posted 02/27/2020
    Reconstructed human epidermis tissues were embedded in paraffin. After successives baths in xylene and ethanol, antigen was retrieved using warm citrate (for 30 min). After a step of saturation, specimens were incubated overnight with the primary antibody (1:100 dilution).
    Human/Mouse Ki67/MKI67 Antibody MAB7617
  • Human Ki-67/MKI67 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Prostate cancer
    Species: Human
    Verified Customer | Posted 10/26/2017
    1:1000 dilution.
    Human/Mouse Ki67/MKI67 Antibody MAB7617

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