Human Ki67/MKI67 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB7617-100
MAB7617-SP
Ki67/MKI67 in HeLa Human Cell Line.
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Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (2)

Human Ki67/MKI67 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Ki67/MKI67 in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1297A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Human/Mouse Ki67/MKI67 synthetic peptide
Accession # E9PVX6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Simple Western
20 µg/mL
HeLa human cervical epithelial carcinoma cell line and MCF‑7 human breast cancer cell line
Immunohistochemistry
3-25 µg/mL
See below
Dual RNAscope ISH-IHC
5-25 µg/mL
Formalin-fixed paraffin-embedded tissue sections of human breast cancer
Immunocytochemistry
0.3-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below
Knockout Validated
Ki67/MKI67 is specifically detected in Hela human cervical epithelial carcinoma parental cell line but is not detectable in Ki67/MKI67 knockout HeLa cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Ki67/MKI67 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

Ki67/MKI67 in HeLa Human Cell Line. Ki67/MKI67 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human Ki67/MKI67 Monoclonal Antibody (Catalog # MAB7617) at 0.3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry Ki67/MKI67 antibody in Human Pancreatic Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

Ki67/MKI67 in Human Pancreatic Cancer Tissue. Ki67/MKI67 was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Rabbit Anti-Human Ki67/MKI67 Monoclonal Antibody (Catalog # MAB7617) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Intracellular Staining by Flow Cytometry Detection of Ki67/MKI67 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of Ki67/MKI67 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either A) untreated or (B) treated with 5 µg/mL PHA for 5 days were stained with Rabbit Anti-Human Ki67/MKI67 Monoclonal Antibody (Catalog # MAB7617) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (FAB100A). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Simple Western View Larger

Detection of Human Ki67/MKI67 by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and MCF‑7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Ki67/MKI67 at approximately 320 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human Ki67/MKI67 Monoclonal Antibody (Catalog # MAB7617). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.

Knockout Validated Ki67/MKI67 Antibody Specificity is Shown by Immunocytochemistry antibody in Knockout Cell Line. View Larger

Ki67/MKI67 Specificity is Shown by Immunocytochemistry in Knockout Cell Line. Ki67/MKI67 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line but is not detected in Ki67/MKI67 knockout (KO) HeLa cell line using Rabbit Anti-Human Ki67/MKI67 Monoclonal Antibody (Catalog # MAB7617) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Knockout Validated View Larger

Human Ki67/MKI67 Specificity Shown by Simple WesternTM in Knockout Cell Line. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and Ki67 knockout HeLa cell line (KO), loaded at 0.2 mg/mL. A specific band was detected for Ki67/MKI67 at approximately 320 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human Ki67/MKI67 Monoclonal Antibody (Catalog # MAB7617). GAPDH (MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ki67/MKI67

MKI67 (also Ki67) is a 350-400 kDa nuclear protein that belongs to a molecular group comprised of mitotic chromosome-associated proteins. Ki67 was originally recognized as an antigen associated with the monoclonal Ki67 antibody raised against Hodgkin's lymphoma nuclear material. Ki-67 is contextually expressed, being potentially found in all cells that are not in the Go phase of the cell cycle. Thus, MKI67 qualifies as a cell proliferation marker. Functionally, Ki67 is known to interact with 160 kDa Hklp2, a protein that promotes centrosome separation and spindle bipolarity. It also directly interacts with NIFK, and apparently binds to UBF, thus playing a role in rRNA synthesis. Human MKI67 is 3256 amino acids (aa) in length. It contains one FHA domain (aa 8-98), followed by at least 24 utilized Ser/Thr phosphorylation sites and sixteen 120 aa repeats (aa 1000-2928) that are interspersed with at least 90 additional utilized phosphorylation sites. There are two potential isoform variants. One isoform is 315-345 kDa in size and shows a deletion of aa 136-495, while a second isoform contains a 58 aa substitution for aa 1-513. Over aa 3120-3256, human Ki67 shares 46% aa sequence identity with the mouse ortholog to Ki67.

Long Name
Antigen Identified by Monoclonal Antibody Ki67
Entrez Gene IDs
4288 (Human); 17345 (Mouse); 291234 (Rat)
Alternate Names
antigen identified by monoclonal Ki-67; Antigen Ki67; antigen KI-67; Ki67; Ki-67; KIA; Marker Of Proliferation Ki-67; MIB-; MIB-1; MKI67; PPP1R105; Proliferation Marker Protein Ki-67; proliferation-related Ki-67 antigen; Protein Phosphatase 1; Regulatory Subunit 105; TSG126

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Citations for Human Ki67/MKI67 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Addition of Chk1 inhibitor and BMP4 cooperatively promotes retinal tissue formation in self-organizing human pluripotent stem cell differentiation culture
    Authors: S Yamasaki, A Kuwahara, A Kishino, T Kimura, M Takahashi, M Mandai
    Regenerative Therapy, 2022;19(0):24-34.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Phenanthroindolizidine Alkaloids Isolated from Tylophora ovata as Potent Inhibitors of Inflammation, Spheroid Growth, and Invasion of Triple-Negative Breast Cancer
    Authors: I Reimche, H Yu, NP Ariantari, Z Liu, K Merkens, S Rotfu beta, K Peter, U Jungwirth, N Bauer, F Kiefer, JM Neudörfl, HG Schmalz, P Proksch, N Teusch
    International Journal of Molecular Sciences, 2022;23(18):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC/IF
  3. Development and Radiation Response Assessment in A Novel Syngeneic Mouse Model of Tongue Cancer: 2D Culture, 3D Organoids and Orthotopic Allografts
    Authors: VK Vincent-Ch, M Seshadri
    Cancers (Basel), 2020;12(3):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Alpha1-antitrypsin protects lung cancer cells from staurosporine-induced apoptosis: the role of bacterial lipopolysaccharide
    Authors: N Schwarz, S Tumpara, S Wrenger, E Ercetin, J Hamacher, T Welte, S Janciauski
    Sci Rep, 2020;10(1):9563.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Reviews for Human Ki67/MKI67 Antibody

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Human Ki67/MKI67 Antibody
By Agnès Tessier on 02/27/2020
Application: ICC/IF Sample Tested: Skin tissue Species: Human

Reconstructed human epidermis tissues were embedded in paraffin. After successives baths in xylene and ethanol, antigen was retrieved using warm citrate (for 30 min). After a step of saturation, specimens were incubated overnight with the primary antibody (1:100 dilution).


Human Ki-67/MKI67 Antibody
By Anonymous on 10/26/2017
Application: IHC Sample Tested: Prostate cancer Species: Human

1:1000 dilution.