Human Lamin B1 Antibody

Catalog # Availability Size / Price Qty
MAB8525-100
MAB8525-SP
Detection of Human Lamin B1 by Western Blot.
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Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (1)

Human Lamin B1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Lamin B1 in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 919007
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Lamin B1
Arg418-Ser582
Accession # P20700
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
3-25 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Lamin B1 antibody by Western Blot. View Larger

Detection of Human Lamin B1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and MOLT-4 human acute lymphoblastic leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Lamin B1 Monoclonal Antibody (Catalog # MAB8525) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Lamin B1 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Lamin B1 antibody in Human Liver by Immunohistochemistry (IHC-P). View Larger

Lamin B1 in Human Liver. Lamin B1 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Lamin B1 Monoclonal Antibody (Catalog # MAB8525) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclear membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunocytochemistry Lamin B1 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

Lamin B1 in HeLa Human Cell Line. Lamin B1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Lamin B1 Monoclonal Antibody (Catalog # MAB8525) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclear membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lamin B1

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Lamin B1 is a heavily phosphorylated protein, with a calculated molecular weight of 66kDa.

Entrez Gene IDs
4001 (Human); 16906 (Mouse)
Alternate Names
ADLD; Lamin B1; lamin-B1; LMN; LMN2; LMNB; LMNB1; MGC111419

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Citations for Human Lamin B1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. G Protein-Coupled Receptor 37L1 Modulates Epigenetic Changes in Human Renal Proximal Tubule Cells
    Authors: I Armando, S Cuevas, C Fan, M Kumar, Z Izzi, PA Jose, PR Konkalmatt
    International Journal of Molecular Sciences, 2022-11-21;23(22):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Identification of a novel tumor angiogenesis inhibitor targeting Shh/Gli1 signaling pathway in Non-small cell lung cancer
    Authors: X Lei, Y Zhong, L Huang, S Li, J Fu, L Zhang, Y Zhang, Q Deng, X Yu
    Cell Death Dis, 2020-04-14;11(4):232.
    Species: Mouse
    Sample Types: Protein
    Applications: Western Blot

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Reviews for Human Lamin B1 Antibody

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Human Lamin B1 Antibody
By Anonymous on 02/10/2022
Application: IHC Sample Tested: Pituitary gland tissue Species: Human