Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 919007
View all Lamin B1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Lamin B1
Arg418-Ser582
Accession # P20700

Specificity

Detects human Lamin B1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Lamin B1 Antibody

Detection of Human Lamin B1 antibody by Western Blot.

Detection of Human Lamin B1 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and MOLT-4 human acute lymphoblastic leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Lamin B1 Monoclonal Antibody (Catalog # MAB8525) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Lamin B1 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Lamin B1 antibody in Human Liver by Immunohistochemistry (IHC-P).

Lamin B1 in Human Liver.

Lamin B1 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Lamin B1 Monoclonal Antibody (Catalog # MAB8525) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclear membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Lamin B1 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

Lamin B1 in HeLa Human Cell Line.

Lamin B1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Lamin B1 Monoclonal Antibody (Catalog # MAB8525) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclear membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Lamin B1 by Western Blot

Detection of Lamin B1 by Western Blot

LCZ696 suppresses LPS-induced activation of the TLR4/Myd88 pathway in HUVECs.Cells were stimulated with LPS (1 μg/mL) in the presence or absence of LCZ696 (20 μM) for 24 hours. (A). The levels of TLR4 and Myd88 were measured using Western blot; (B). Nuclear levels of NF-kappa B p65 (***P < 0.005 vs. control group; ##, ###P < 0.01, 0.005 vs. LPS group). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33839697), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Lamin B1 by Western Blot

Detection of Lamin B1 by Western Blot

Pristimerin suppresses endothelial cellular motilities required for angiogenesis through inactivating Shh/Gli1 signaling pathway. A Pristimerin suppressed nucleus translocation of Gli1 in endothelial cells. After incubating with Shh (100 ng/mL) and pristimerin (125, 250, and 500 nM) for 24 h, the HUVECs and HMEC-1 cells were stained with Gli1 antibody and Alexa Fluor 488 Donkey anti-Rabbit IgG. DAPI was used to label the nucleus. The cells were observed and photographed with a laser scanning confocal microscope. Scale bar: 20 μm. b, c Pristimerin inhibits Gli1 distribution in endothelial cells. The HUVECs and HMEC-1 cells were incubated with Shh and pristimerin for 24 h, and then lysed with a NE-PERTM Nucleus and Cytoplasmic Extraction Kit. After that, proteins were subjected to western blotting assay. beta -actin and Lamin B were set as loading control. The quantitative data were presented as ratios of Gli1/ beta -actin and Gli1/Lamin B. The representative blot and quantitative data were shown in B&C, respectively. d, e Pristimerin inactivated Shh/Gli1 and its downstream signaling pathway. The cells were treated with Shh and pristimerin for 24 h and lysed using RIPA buffer. Total proteins were subjected to western blotting assay. The representative blot and quantitative data were shown in d, e, respectively. The data are presented as mean ± SEM, n = 3. ***P < 0.001 compared with the control group; #P < 0.05 and ###P < 0.001 compared with the SHH-treated group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32286274), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Lamin B1 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Western Blot

2 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and MOLT‑4 human acute lymphoblastic leukemia cell line

Reviewed Applications

Read 1 review rated 5 using MAB8525 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Lamin B1

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Lamin B1 is a heavily phosphorylated protein, with a calculated molecular weight of 66kDa.

Alternate Names

ADLD, LMN2, LMNB1

Entrez Gene IDs

4001 (Human); 16906 (Mouse)

Gene Symbol

LMNB1

UniProt

P20700

Additional Lamin B1 Products

Product Documents for Human Lamin B1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Lamin B1 Antibody

For research use only

Related Research Areas

Cancer Biomarkers
Epigenetics

Citations for Human Lamin B1 Antibody

Customer Reviews for Human Lamin B1 Antibody (1)

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  • Human Lamin B1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Pituitary gland tissue
    Species: Human
    Verified Customer | Posted 02/10/2022
    Human Lamin B1 Antibody MAB8525

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Protocols

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