Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Xenograft

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 320416
View all LIM1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human LIM1
Leu265-Trp406
Accession # P48742

Specificity

Detects human LIM1 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human LIM1 Antibody

LIM1 in Human Brain (Cerebellum).

LIM1 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Mouse Anti-Human LIM1 Monoclonal Antibody (Catalog # MAB2725) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in Purkinje neurons. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Human LIM1 by Western Blot

Detection of Human LIM1 by Western Blot

LIM1 promoted CREB phosphorylation in EC. (A) Volcano plot of DEGs in LIM1-KD (#1 and #2) cells versus Ctrl cells generated from RNA-seq results. (B) The highly ranked GO cluster among commonly downregulated genes in LIM1-KD cells compared with Ctrl cells by Ingenuity Pathway Analysis (IPA). (C) Western blotting of LIM1, ERK, phospho-ERK, CREB, phospho-CREB, and GAPDH using Ctrl cells and LIM1-KD cells. (D) Relative intensities of Akt, phospho-Akt, ERK, phospho-ERK, CREB, phospho-CREB bands by western blot analysis. Signals intensities were normalized to the GAPDH intensity in the same samples and then the intensity of the LIM1-KD (#1 and #2) cells were normalized to Ctrl cells [n = 5 (ERK, p-ERK, CREB, p-ERK), n = 4 (Akt, p-Akt) one-way ANOVA]. (E) Immunofluorescent staining for phospho-CREB and human vimentin in sections of xenograft tumors derived from Ctrl cells and LIM1-KD cells. Red: phosphorylated CREB, blue: nucleus, green: human vimentin. (F) Intensity of phospho-CREB in the tumor region (n = 4, one-way ANOVA). *p < 0.05, **p < 0.01. Graphs show means ± standard deviations. (G) MTT assay to assess cell proliferation. HEC50B cells were treated with CREB inhibitors, KG501 and compoun3i, respectively. Red, orange, green and blue indicate inhibitor concentrations at 0.5 μM, 1.0 μM, 5.0 μM and 10 μM, respectively. (n = 5, two-way ANOVA *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36969081), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human LIM1 by Western Blot

Detection of Human LIM1 by Western Blot

LIM1 promoted CREB phosphorylation in EC. (A) Volcano plot of DEGs in LIM1-KD (#1 and #2) cells versus Ctrl cells generated from RNA-seq results. (B) The highly ranked GO cluster among commonly downregulated genes in LIM1-KD cells compared with Ctrl cells by Ingenuity Pathway Analysis (IPA). (C) Western blotting of LIM1, ERK, phospho-ERK, CREB, phospho-CREB, and GAPDH using Ctrl cells and LIM1-KD cells. (D) Relative intensities of Akt, phospho-Akt, ERK, phospho-ERK, CREB, phospho-CREB bands by western blot analysis. Signals intensities were normalized to the GAPDH intensity in the same samples and then the intensity of the LIM1-KD (#1 and #2) cells were normalized to Ctrl cells [n = 5 (ERK, p-ERK, CREB, p-ERK), n = 4 (Akt, p-Akt) one-way ANOVA]. (E) Immunofluorescent staining for phospho-CREB and human vimentin in sections of xenograft tumors derived from Ctrl cells and LIM1-KD cells. Red: phosphorylated CREB, blue: nucleus, green: human vimentin. (F) Intensity of phospho-CREB in the tumor region (n = 4, one-way ANOVA). *p < 0.05, **p < 0.01. Graphs show means ± standard deviations. (G) MTT assay to assess cell proliferation. HEC50B cells were treated with CREB inhibitors, KG501 and compoun3i, respectively. Red, orange, green and blue indicate inhibitor concentrations at 0.5 μM, 1.0 μM, 5.0 μM and 10 μM, respectively. (n = 5, two-way ANOVA *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36969081), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human LIM1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebellum)

Western Blot

1 µg/mL
Sample: Recombinant Human LIM1

Reviewed Applications

Read 1 review rated 4 using MAB2725 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LIM1

LIM homeobox 1 gene product (LIM1), also known as LHX-1, is a homeobox nuclear transcription factor expressed in human brain, thymus, tonsil and many myeloid leukemias. The 406 amino acid human LIM1 contains a LIM domain with twin zinc-binding sequences and a homeobox DNA-binding domain. The C-terminal sequence used as an immunogen is virtually identical between human and mouse LIM1. A truncated human isoform does not include this sequence. LIM1 activity is controlled by interaction with the ubiquitous nuclear adaptor protein Ldb-1 (Lim-domain-binding protein-1) which binds the LIM domain and mediates dimerization.

Long Name

LIM Homeobox 1

Alternate Names

LHX1

Entrez Gene IDs

3975 (Human)

Gene Symbol

LHX1

UniProt

P48742

Additional LIM1 Products

Product Documents for Human LIM1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human LIM1 Antibody

For research use only

Citations for Human LIM1 Antibody

Customer Reviews for Human LIM1 Antibody (1)

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  • Human LIM1 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 03/17/2021
    Human LIM1 Antibody MAB2725

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