Human LKB1/STK11 Antibody Summary
Accession # Q15831
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human LKB1/STK11 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human LKB1/STK11 Monoclonal Antibody (Catalog # MAB8055) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for LKB1/STK11 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western Blot Shows Human LKB1/STK11 Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and LKB1/STK11 knockout HEK293T cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human LKB1/STK11 Monoclonal Antibody (Catalog # MAB8055) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for LKB1/STK11 at approximately 55 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
LKB1, also known as STK11 and JBS, is a 55 kDa member of the CAMK Ser/Thr protein kinase family. It is ubiquitously expressed, and considered to be a tumor suppressor. LKB1 is both nuclear and cytoplasmic, and appears to contribute to cell polarization, G1 cell cycle arrest and Wnt signaling. It is known to complex with STRAD and CAB39/MO25, and phosphorylate PTEN plus p53. Human LKB1 is 433 amino acids (aa) in length. It contains a protein kinase domain (aa 49‑309) and a prenylation motif (CysLysGlnGln) over aa 430‑433. Phosphorylation on Ser428 promotes the ability of LKB1 to suppress G361 cell growth. There are two potential isoforms of LKB1. One shows a nine aa insertion after Tyr126, while another shows a 34 aa substitution for aa 371‑433. Human LKB1 shares 90% aa sequence identity with mouse and rat LKB1.
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