Human LKB1/STK11 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human LKB1/STK11 in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG2A Clone # 873502
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human LKB1/STK11
    Met1-Gln433
    Accession # Q15831
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human LKB1/STK11 by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human LKB1/STK11 Monoclonal Antibody (Catalog # MAB8055) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for LKB1/STK11 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LKB1/STK11
LKB1, also known as STK11 and JBS, is a 55 kDa member of the CAMK Ser/Thr protein kinase family. It is ubiquitously expressed, and considered to be a tumor suppressor. LKB1 is both nuclear and cytoplasmic, and appears to contribute to cell polarization, G1 cell cycle arrest and Wnt signaling. It is known to complex with STRAD and CAB39/MO25, and phosphorylate PTEN plus p53. Human LKB1 is 433 amino acids (aa) in length. It contains a protein kinase domain (aa 49‑309) and a prenylation motif (CysLysGlnGln) over aa 430‑433. Phosphorylation on Ser428 promotes the ability of LKB1 to suppress G361 cell growth. There are two potential isoforms of LKB1. One shows a nine aa insertion after Tyr126, while another shows a 34 aa substitution for aa 371‑433. Human LKB1 shares 90% aa sequence identity with mouse and rat LKB1.
  • Long Name:
    Serine/threonine Protein Kinase LKB1
  • Entrez Gene IDs:
    6794 (Human); 20869 (Mouse); 314621 (Rat)
  • Alternate Names:
    EC 2.7.11.1; LKB1 serine/threonine kinase 11 (Peutz-Jeghers syndrome); LKB1; PJS polarization-related protein LKB1; PJS; Renal carcinoma antigen NY-REN-19; serine/threonine kinase 11; serine/threonine-protein kinase 11; Serine/threonine-protein kinase LKB1; STK11
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A Isotype Control

Ctrl MAB003 32  
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Mouse IgG2A Isotype Control

Ctrl MAB0031 7  
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Mouse IgG2A Isotype Control

Ctrl MAB003R
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rat Anti-Mouse IgG2A PE-conjugated Antibody

Flow F0129 1  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Rat Anti-Mouse IgG2A PerCP-conjugated Antibody

Flow F0131  
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Mouse IgG2A Antibody

Flow MAB0032  
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Rat Anti-Mouse IgG2A APC-conjugated Antibody

Flow F0130  
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