Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair)

Cited:

ELISA Capture, Luminex Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 21113
View all M-CSF Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human M-CSF

Specificity

Detects recombinant human (rh)M-CSF in Western blots. In sandwich ELISAs, less than 7% cross-reactivity was observed with rmM-CSF. No significant cross-reactivity or interference was observed with over 75 other cytokines tested.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human M‑CSF Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human M-CSF (Catalog # 216-MC)
under non-reducing conditions only

Human M-CSF Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human M‑CSF Biotinylated Antibody (Catalog # BAF216)
  • Standard: Recombinant Human M-CSF Protein (Catalog # 216-MC)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using MAB616 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: M-CSF

M-CSF (CSF-1) is the primary regulator of macrophage survival, proliferation and differentiation. It is essential for macrophage-related functions such as bone resorption, vascular development, and innate immunity. It signals through M-CSF R on monocytes, tissue macrophages, and osteoblasts. Both M-CSF and its receptor can be expressed by tumor cells, allowing M-CSF to act as an autocrine growth factor for cancer cells. Full length M-CSF is expressed as a transmembrane dimer, but various additional forms can be generated by alternative splicing, differential glycosylation, chondroitin sulfate proteoglycan modification, and proteolytic shedding.

Long Name

Macrophage Colony Stimulating Factor

Alternate Names

CSF-1, CSF1, Lanimostim, MCSF

Entrez Gene IDs

1435 (Human); 12977 (Mouse)

Gene Symbol

CSF1

Additional M-CSF Products

Product Documents for Human M‑CSF Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human M‑CSF Antibody

For research use only

Citations for Human M‑CSF Antibody

Customer Reviews for Human M‑CSF Antibody (2)

5 out of 5
2 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

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  • Human M-CSF Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 07/23/2019
    Human M‑CSF Antibody MAB616
  • Human M-CSF Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 11/03/2017
    This antibody was used as a capture antibody in the development of a sandwich ELISA. The R&D systems polyclonal antibody AF216 was used as the detection antibody. M-CSF was measured in human serum samples with this assay.
    Human M‑CSF Antibody MAB616

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