MDM2 is a key regulator of p53 tumor suppressor protein activity and stability. MDM2 binds to and inhibits the transactivation domain of p53. In addition, MDM2 controls p53 stability by functioning as its E3 ligase in ubiquitination and by shuttling p53 from the nucleus to the cytoplasm for subsequent degradation. The importance of the p53/MDM2 relationship is underscored by the existence of an autoregulatory feedback loop whereby activated p53 transcriptionally upregulates the expression of its own inhibitor, MDM2.
Human MDM2/HDM2 Antibody (197702)
R&D Systems | Catalog # MAB1244
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Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 197702
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Product Specifications
Immunogen
E. coli-derived recombinant human MDM2/HDM2
Asn3-Pro491
Accession # Q00987
Asn3-Pro491
Accession # Q00987
Specificity
Detects human MDM2/HDM2.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human MDM2/HDM2 Antibody (197702)
Immunoprecipitation of Human MDM2.
U2OS human osteosarcoma cell line was untreated (-) or treated (+) with proteasome inhibitor LLnV for 1 hour. MDM2 was immunoprecipitated from 500 µg of cell lysate following incubation with 2-3 µg Mouse Anti-Human MDM2 Monoclonal Antibody (Catalog # MAB1244) for 1 hour on ice. MDM2-antibody complexes were absorbed using goat anti-mouse agarose (Sigma). Immunoprecipitated MDM2 was detected by Western blot using 1 µg/mL Human/Mouse/Rat MDM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1244). View our recommended buffer recipes for immunoprecipitation.Applications for Human MDM2/HDM2 Antibody (197702)
Application
Recommended Usage
Immunoprecipitation
2.5 µg/500 µg cell lysate
Sample: U2OS human osteosarcoma cell line treated with proteasome inhibitor LLnV, see our available Western blot detection antibodies
Sample: U2OS human osteosarcoma cell line treated with proteasome inhibitor LLnV, see our available Western blot detection antibodies
Reviewed Applications
Read 3 reviews rated 5 using MAB1244 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: MDM2/HDM2
Long Name
Double Minute 2 Protein
Alternate Names
HDM2
Gene Symbol
MDM2
UniProt
Additional MDM2/HDM2 Products
Product Documents for Human MDM2/HDM2 Antibody (197702)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human MDM2/HDM2 Antibody (197702)
For research use only
Related Research Areas
Citations for Human MDM2/HDM2 Antibody (197702)
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Customer Reviews for Human MDM2/HDM2 Antibody (197702) (3)
5 out of 5
3 Customer Ratings
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Application: Western BlotSample Tested: Purified proteinSpecies: HumanVerified Customer | Posted 12/04/2019
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Application: Western BlotSample Tested: Prostate cancerSpecies: HumanVerified Customer | Posted 01/17/2018
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Application: Western BlotSample Tested: Human medulloblastoma cellsSpecies: HumanVerified Customer | Posted 03/09/2016
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Protocols
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Associated Pathways
