Human MDM2/HDM2 Antibody

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  • Species Reactivity
    Human
  • Specificity
    Detects human MDM2/HDM2.
  • Source
    Monoclonal Mouse IgG2B Clone # 197702
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human MDM2/HDM2
    Asn3-Pro491
    Accession # Q00987
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunoprecipitation
    2.5 µg/500 µg cell lysate
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Immunoprecipitation
Immunoprecipitation of Human MDM2. U2OS human osteosarcoma cell line was untreated (-) or treated (+) with proteasome inhibitor LLnV for 1 hour. MDM2 was immunoprecipitated from 500 μg of cell lysate following incubation with 2‑3 µg Mouse Anti-Human MDM2 Monoclonal Antibody (Catalog # MAB1244) for 1 hour on ice. MDM2-antibody complexes were absorbed using goat anti-mouse agarose (Sigma). Immunoprecipitated MDM2 was detected by Western blot using 1 µg/mL Human/Mouse/Rat MDM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1244). View our recommended buffer recipes for immunoprecipitation.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MDM2/HDM2

MDM2 is a key regulator of p53 tumor suppressor protein activity and stability. MDM2 binds to and inhibits the transactivation domain of p53. In addition, MDM2 controls p53 stability by functioning as its E3 ligase in ubiquitination and by shuttling p53 from the nucleus to the cytoplasm for subsequent degradation. The importance of the p53/MDM2 relationship is underscored by the existence of an autoregulatory feedback loop whereby activated p53 transcriptionally upregulates the expression of its own inhibitor, MDM2.

  • Long Name:
    Double Minute 2 Protein
  • Entrez Gene IDs:
    4193 (Human); 17246 (Mouse)
  • Alternate Names:
    ACTFS; HDM2; HDMX; MDM2 oncogene, E3 ubiquitin protein ligase; MDM2
Related Research Areas

FAQs

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ExactaChIP Chromatin Immunoprecipitation Kits
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ExactaChIP Chromatin IP Buffer Panel

ChIP ECP001 2
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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Isotype Control

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Mouse IgG2B Isotype Control

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Mouse IgG2B Isotype Control

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Reconstitution Buffers
Description Application Cat# Citations Images  

Reconstitution Buffer 1 (PBS)

RB01
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 32  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow , IHC , ICC NL007 10
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 4  
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG Antibody

WB AF007 4
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133 1  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Rat Anti-Mouse IgG2B PE-conjugated Antibody

Flow F0132  
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Mouse IgG2B Antibody

Flow MAB0043  
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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

Flow F0134 1
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Western Blot.

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Images Ratings Applications Species Reviewed By Date Details
Western Blot Human MDM2/HDM2 Antibody MAB1244 Array MAB1244
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Excellent
 WB Human Anonymous 01/17/2018
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Western Blot Human MDM2/HDM2 Antibody MAB1244
Western Blot: Human MDM2/HDM2 Antibody [MAB1244].

Summary

Application Western Blot
Sample Tested Prostate cancer
Species Human

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