Human MKK4 Antibody

Catalog # Availability Size / Price Qty
MAB3390
MAB3390-SP
Human MKK4 Antibody in Data
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Product Details
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Reviews (4)

Human MKK4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human MKK4 in direct ELISAs. This antibody does not cross-react with recombinant human MKK7.
Source
Monoclonal Mouse IgG2B Clone # 323042
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human MKK4
Met1-Asp399
Accession # P45985
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
3-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry MKK4 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

MKK4 in HeLa Human Cell Line. MKK4 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human MKK4 Monoclonal Antibody (Catalog # MAB3390) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MKK4

Mitogen-activated protein kinase kinase 4 (MKK4 or MAP2K4), also known as SAPK/ERK kinase 1 (SEK1) and Jun kinase kinase 1 (JNKK1), is activated by proinflammatory cytokines and environmental stresses. Activation occurs through phosphorylation at Ser257 and Thr261 by several upstream MAPK kinase kinases (MAP3Ks). MKK4 is a dual specificity protein kinase, phosphorylating and activating the JNK family of MAP kinases at Thr and Tyr positions within the phosphoacceptor sequence Thr-Pro-Tyr.

Long Name
Mitogen-activated Protein Kinase Kinase 4
Entrez Gene IDs
6416 (Human)
Alternate Names
c-Jun N-terminal kinase kinase 1; dual specificity mitogen-activated protein kinase kinase 4; JNK activating kinase 1; JNK-activated kinase 1; JNK-activating kinase 1; JNKK; JNKK1; JNKK1MAPK/ERK kinase 4; MAP kinase kinase 4; MAP2K4; MAPKK4; MEK4; MEK4MEK 4; mitogen-activated protein kinase kinase 4; MKK4; MKK4SAPK/ERK kinase 1; PRKMK4; PRKMK4MAPKK 4; SEK1; SERK1; SERK1EC 2.7.12.2

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Cell and Tissue Staining Kits

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human MKK4 Antibody

Average Rating: 3.8 (Based on 4 Reviews)

5 Star
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4 Star
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3 Star
25%
2 Star
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Human MKK4 Antibody
By Anonymous on 01/14/2021
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human MKK4 Antibody
By Anonymous on 02/07/2020
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma


Human MKK4 Antibody
By Anonymous on 11/14/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human MKK4 Antibody
By Anonymous on 11/11/2018
Application: ELISA Sample Tested: Serum and Plasma Species: Human