Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Knockout Validated, Immunohistochemistry, Western Blot, ELISA Capture (Matched Antibody Pair), Simple Western

Cited:

Immunohistochemistry, Western Blot, ELISA Development, ELISA Microarray Development

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all MMP-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MMP-1
Phe20-Asn469
Accession # P03956

Specificity

Detects human MMP-1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross-reactivity with recombinant human (rh) MMP‑2, rhMMP-3, rhMMP-7, rhMMP-8, rhMMP-9, rhMMP-10, rhMMP-12, rhMMP-13, and rhMMP-16 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human MMP‑1 Antibody

Detection of Human MMP-1 antibody by Western Blot.

Detection of Human MMP‑1 by Western Blot.

Western blot shows lysates of PC-3 human prostate cancer cell line and HEK001 human epidermal keratinocyte cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF901) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MMP-1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human MMP‑1 by Simple WesternTM.

Left: Simple Western lane view shows lysates of MDA‑MB‑231 human breast cancer cell line, loaded at 0.1 mg/ml. A specific band was detected for MMP‑1 at approximately 55 kDa (as indicated) using both 10 µg/ml and 50 µg/ml of Goat Anti-Human MMP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF901) followed by HRP-conjugated Donkey Anti-Goat Secondary Antibody (Catalog # 043-522-2). This experiment was conducted under reducing conditions and using the 12-230kDa separation system. Right: Simple Western electropherogram showing the same Goat Anti-Human MMP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF901) tested at 10 µg/ml (blue line) and 50 µg/ml (green line) in the MDA‑MB‑231 human breast cancer cell line.
MMP-1 antibody in Human Ovarian Cancer Tissue by Immunohistochemistry (IHC-P).

MMP‑1 in Human Ovarian Cancer Tissue.

MMP-1 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 15 µg/mL Goat Anti-Human MMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF901) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
MMP-1 antibody in Human Prostate Tissue by Immunohistochemistry (IHC-P).

MMP‑1 in Human Prostate Tissue.

MMP-1 was detected in immersion fixed paraffin-embedded sections of human prostate tissue using Goat Anti-Human MMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF901) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Western Blot Shows Human MMP-1 Antibody Specificity by Using Knockout Cell Line.

Western Blot Shows Human MMP-1 Specificity by Using Knockout Cell Line.

Western blot shows lysates of PC-3 human prostate cancer parental cell line and MMP-1 knockout PC-3 cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF901) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MMP-1 at approximately 50 kDa (as indicated) in the parental PC-3 cell line, but is not detectable in knockout PC-3 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human MMP‑1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human ovarian cancer tissue and immersion fixed paraffin-embedded sections of human prostate

Knockout Validated

MMP-1 is specifically detected in PC‑3 human prostate cancer parental cell line but is not detectable in MMP-1 knockout PC‑3 cell line.

Simple Western

10-50 µg/mL
Sample: MDA-MB-231 human breast cancer cell line

Western Blot

1 µg/mL
Sample: PC‑3 human prostate cancer cell line and HEK001 human epidermal keratinocyte cell line

Human MMP-1 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 0.2-0.8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human MMP‑1 Biotinylated Antibody (Catalog # BAF901)
  • Standard: Recombinant Human MMP-1 Protein, CF (Catalog # 901-MP)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4 using AF901 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MMP-1

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-1 (interstitial collagenase), can degrade a broad range of substrates including types I, II, III, VII, VIII, and X collagens as well as casein, gelatin,
alpha -1 antitrypsin, myelin basic protein, L-Selectin, pro-TNF, IL-1 beta, IGFBP-3, IGFBP-5, pro-MMP-2, and pro-MMP-9. A significant role of MMP-1 is the degradation of fibrillar collagens in extracellular matrix remodeling, characterized by the cleavage of the interstitial collagen triple helix into ¾, ¼ fragments. However, as the list of substrates above illustrates, the role of MMP-1 is more diverse than originally envisaged, and may involve enzyme cascades, cytokine regulation, and cell surface molecule modulation. MMP-1 is expressed by fibroblasts, keratinocytes, endothelial cells, monocytes, and macrophages. Structurally, MMP-1 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain.

References

  1. Cawston, T.E. (2004) in Interstitial Collagenase. Barrett, A.J. et al. (eds): Handbook of Proteolytic Enzymes, San Diego: Academic Press, p. 472.

Long Name

Matrix Metalloproteinase 1

Alternate Names

MMP1

Entrez Gene IDs

4312 (Human); 83995 (Mouse)

Gene Symbol

MMP1

UniProt

P03956

Additional MMP-1 Products

Product Documents for Human MMP‑1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human MMP‑1 Antibody

For research use only

Citations for Human MMP‑1 Antibody

Customer Reviews for Human MMP‑1 Antibody (2)

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  • Human MMP-1 Antibody
    Name: Joseph Moore
    Application: Immunohistochemistry
    Sample Tested: Cartilage tissue and Bone Extracts
    Species: Human
    Verified Customer | Posted 07/12/2019
    These samples were IHC-P, and the antibody was diluted 1:100 in BSA blocking solution. We used a HRP conjugate.
    Human MMP‑1 Antibody AF901
  • Human MMP-1 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 11/09/2017
    The antibody AF901 was used as both the capture and detection molecule in an ELISA to measure MMP-1 in human plasma samples.
    Human MMP‑1 Antibody AF901

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Protocols

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