Human MMP-1 Antibody

Catalog # Availability Size / Price Qty
AF901
AF901-SP
Detection  of Human MMP‑1 by Western Blot.
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Product Details
Citations (7)
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Reviews (1)

Human MMP-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human MMP-1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross-reactivity with recombinant human (rh) MMP‑2, rhMMP-3, rhMMP-7, rhMMP-8, rhMMP-9, rhMMP-10, rhMMP-12, rhMMP-13, and rhMMP-16 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MMP-1
Phe20-Asn469
Accession # P03956
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Knockout Validated
MMP-1 is specifically detected in PC‑3 human prostate cancer parental cell line but is not detectable in MMP-1 knockout PC‑3 cell line.
 

Human MMP-1 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Human MMP‑1 Biotinylated Antibody (Catalog # BAF901)

Standard: Recombinant Human MMP-1 Protein, CF (Catalog # 901-MP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human MMP‑1 by Western Blot. View Larger

Detection of Human MMP‑1 by Western Blot. Western blot shows lysates of PC‑3 human prostate cancer cell line and HEK001 human epidermal keratinocyte cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MMP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF901) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MMP‑1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry MMP‑1 in Human Ovarian Cancer Tissue. View Larger

MMP‑1 in Human Ovarian Cancer Tissue. MMP‑1 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 15 µg/mL Goat Anti-Human MMP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF901) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry MMP‑1 in Human Prostate Tissue. View Larger

MMP‑1 in Human Prostate Tissue. MMP‑1 was detected in immersion fixed paraffin-embedded sections of human prostate tissue using Goat Anti-Human MMP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF901) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Knockout Validated Western Blot Shows Human MMP-1 Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human MMP-1 Specificity by Using Knockout Cell Line. Western blot shows lysates of PC‑3 human prostate cancer parental cell line and MMP-1 knockout PC‑3 cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MMP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF901) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MMP‑1 at approximately 50 kDa (as indicated) in the parental PC-3 cell line, but is not detectable in knockout PC-3 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-1

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-1 (interstitial collagenase), can degrade a broad range of substrates including types I, II, III, VII, VIII, and X collagens as well as casein, gelatin,
alpha -1 antitrypsin, myelin basic protein, L-Selectin, pro-TNF, IL-1 beta, IGFBP-3, IGFBP-5, pro-MMP-2, and pro-MMP-9. A significant role of MMP-1 is the degradation of fibrillar collagens in extracellular matrix remodeling, characterized by the cleavage of the interstitial collagen triple helix into ¾, ¼ fragments. However, as the list of substrates above illustrates, the role of MMP-1 is more diverse than originally envisaged, and may involve enzyme cascades, cytokine regulation, and cell surface molecule modulation. MMP-1 is expressed by fibroblasts, keratinocytes, endothelial cells, monocytes, and macrophages. Structurally, MMP-1 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain.

References
  1. Cawston, T.E. (2004) in Interstitial Collagenase. Barrett, A.J. et al. (eds): Handbook of Proteolytic Enzymes, San Diego: Academic Press, p. 472.
Long Name
Matrix Metalloproteinase 1
Entrez Gene IDs
4312 (Human)
Alternate Names
CLGmatrix metalloprotease 1; CLGN; EC 3.4.24; EC 3.4.24.7; Fibroblast collagenase; interstitial collagenase; matrix metallopeptidase 1 (interstitial collagenase); matrix metalloproteinase 1 (interstitial collagenase); Matrix metalloproteinase-1; MMP1; MMP-1

Product Datasheets

Citations for Human MMP-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Protective effect of curcumin against ultraviolet A irradiation?induced photoaging in human dermal fibroblasts
    Authors: X Liu, R Zhang, H Shi, X Li, Y Li, A Taha, C Xu
    Mol Med Rep, 2018;17(5):7227-7237.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis.
    Authors: Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK
    J. Immunol. Methods, 2012;378(1):72-80.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  3. TLR4 Protein Contributes to Cigarette Smoke-induced Matrix Metalloproteinase-1 (MMP-1) Expression in Chronic Obstructive Pulmonary Disease.
    Authors: Geraghty P, Dabo AJ, D'Armiento J
    J. Biol. Chem., 2011;286(34):30211-8.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Increased expression of matrix metalloproteinase-1 in systemic vessels of preeclamptic women: a critical mediator of vascular dysfunction.
    Authors: Estrada-Gutierrez G, Cappello RE, Mishra N, Romero R, Strauss JF, Walsh SW
    Am. J. Pathol., 2011;178(1):451-60.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Development and validation of sandwich ELISA microarrays with minimal assay interference.
    Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
    J. Proteome Res., 2008;7(6):2406-14.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Microarray Development
  6. Guggulsterone blocks IL-1beta-mediated inflammatory responses by suppressing NF-kappaB activation in fibroblast-like synoviocytes.
    Authors: Lee YR, Lee JH, Noh EM, Kim EK, Song MY, Jung WS, Park SJ, Kim JS, Park JW, Kwon KB, Park BH
    Life Sci., 2008;82(23):1203-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Advanced glycation end products increases matrix metalloproteinase-1, -3, and -13, and TNF-alpha in human osteoarthritic chondrocytes.
    Authors: Nah SS, Choi IY, Yoo B, Kim YG, Moon HB, Lee CK
    FEBS Lett., 2007;581(9):1928-32.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot

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Human MMP-1 Antibody
By Anonymous on 11/09/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Human

The antibody AF901 was used as both the capture and detection molecule in an ELISA to measure MMP-1 in human plasma samples.