Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Neutralization, Immunoprecipitation

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 117239
View all MMP-10 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MMP-10
Tyr18-Cys476
Accession # P09238

Specificity

Detects the pro and active forms of human MMP-10 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human MMP-1, -2, -3, -7, -8, -9, -12, or -13 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human MMP‑10 Antibody

Detection of Human MMP-10 by Immunohistochemistry

Detection of Human MMP-10 by Immunohistochemistry

Co-localisation of MMPs with markers of classical activation in human atherosclerotic plaques.(A) Serial sections were stained by peroxidase with anti-CD68 (A. CD68) and by dual immuno-fluorescence with anti-MMP-1 or anti-MMP-10 (red) together with anti-COX-2 or p65RelA (green) as shown, and the images were superimposed digitally. Nuclear localised p65RelA (NF-kB*) was detected because of the shift in nuclear counterstain colour from dark blue (DAPI alone) to sky blue (blue plus green) in the superimposed image. (B) Areas rich in macrophages identified from the peroxidase stain were identified in the serial section. Cells in the whole field were counted and a percentage of each staining pattern calculated. Values are means ± SEM, n = 6, * p<0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22880008), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human MMP‑10 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human intestine

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human MMP‑10 (Catalog # 910‑MP), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human MMP‑10 Western Blot Standard (Catalog # WBC026)

Neutralization

Measured by its ability to neutralize Recombinant Human MMP‑10 (1 µg/mL, Catalog # 910‑MP) cleavage of the fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (10 µM, Catalog # ES002). The Neutralization Dose (ND50) is typically 1 µg/mL.

Reviewed Applications

Read 1 review rated 1 using MAB9101 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MMP-10

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-10 (stromelysin 2) degrades a broad range of substrates including gelatin, collagen types III, IV and V, fibronectin, aggrecan, and pig cartilage proteoglycan. MMP-10 can activate other MMPs such as MMP-1 and MMP-8. MMP-10 is expressed in keratinocytes, T cells, menstrual endometrium, and a few tumor samples. Structurally, MMP-10 may be divided into four distinct domains: a pro-domain which is cleaved upon activation, a catalytic domain containing the zinc binding site; a short linker region, and a carboxyl terminal hemopexin-like domain.

Long Name

Matrix Metalloproteinase 10

Alternate Names

MMP10, Stromelysin 2

Entrez Gene IDs

4319 (Human)

Gene Symbol

MMP10

UniProt

P09238

Additional MMP-10 Products

Product Documents for Human MMP‑10 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human MMP‑10 Antibody

For research use only

Citations for Human MMP‑10 Antibody

Customer Reviews for Human MMP‑10 Antibody (1)

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  • Human MMP-10 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Adult lung
    Species: Human
    Verified Customer | Posted 03/13/2019
    Doesn't work with and without citrate retrieval, Used IgG as negative control and a different MMP10 antibody as a positive control.
    Human MMP‑10 Antibody MAB9101
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to hear that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.

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Protocols

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