Human/Mouse G9a/EHMT2 Antibody

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Product Details
Citations (10)
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Human/Mouse G9a/EHMT2 Antibody Summary

Species Reactivity
Human, Mouse
This antibody specifically recognizes mouse G9a and cross-reacts with human G9a. Not yet tested in other species.
Monoclonal Mouse IgG2A Clone # A8620A
Protein A or G purified from ascites
Recombinant mouse G9a/EHMT2
aa 15-114
A liquid formulation in physiologic saline with 0.1% NaN3.

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Recommended Concentration
Western Blot
This antibody can be used at 0.5 μg/mL with the appropriate secondary reagents to detect mouse G9a.
This antibody can be used at 5 μg/mL with the appropriate secondary reagents to detect mouse G9a.
Optimal dilutions should be determined by each laboratory.
Direct ELISA
This antibody can be used at 0.1 μg/mL with the appropriate secondary reagents to detect mouse G9a.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
This antibody is stable for greater than six months when held at -20° C in a manual defrost freezer or at -70°C. Upon thawing, the antibody can be stored at 2° - 8° C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Background: G9a/EHMT2

Histone H3 Lys 9 (H3-K9) methylation is a crucial epigenetic marker for transcriptional silencing. G9a is the major mammalian H3-K9 methyltransferase that targets euchromatic regions and is essential for mouse embryogenesis.

Long Name
G9a Histone Methyltransferase/Euchromatic Histone-lysine N-methyltransferase 2
Entrez Gene IDs
10919 (Human); 110147 (Mouse)
Alternate Names
ankyrin repeat-containing protein; BAT8; C6orf30; chromosome 6 open reading frame 30; DKFZp686H08213; EC 2.1.1.-; EC; EHMT2; euchromatic histone-lysine N-methyltransferase 2HLA-B-associated transcript 8; FLJ35547; G9A histone methyltransferase; G9a; G9AEm:AF134726.3; H3-K9-HMTase 3; Histone H3-K9 methyltransferase 3; histone-lysine N-methyltransferase, H3 lysine-9 specific 3; HLA-B associated transcript 8; KMT1CNG36/G9a; Lysine N-methyltransferase 1C; NG36; Protein G9a

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Citations for Human/Mouse G9a/EHMT2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Histone Methyltransferase G9a-Promoted Progression of Hepatocellular Carcinoma Is Targeted by Liver-Specific Hsa-miR-122
    Authors: LT Yuan, WJ Lee, YC Yang, BR Chen, CY Yang, MW Chen, JQ Chen, M Hsiao, MH Chien, KT Hua
    Cancers, 2021;13(10):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Inhibition of histone methyltransferase G9a attenuates liver cancer initiation by sensitizing DNA-damaged hepatocytes to p53-induced apoptosis
    Authors: T Nakatsuka, K Tateishi, H Kato, H Fujiwara, K Yamamoto, Y Kudo, H Nakagawa, Y Tanaka, H Ijichi, T Ikenoue, T Ishizawa, K Hasegawa, M Tachibana, Y Shinkai, K Koike
    Cell Death & Disease, 2021;12(1):99.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Cyclin D1 integrates G9a-mediated histone methylation
    Authors: Z Li, X Jiao, G Di Sante, A Ertel, MC Casimiro, M Wang, S Katiyar, X Ju, DV Klopfenste, A Tozeren, W Dampier, I Chepelev, A Jeltsch, RG Pestell
    Oncogene, 2019;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ChIP
  4. Mutual inhibition between HDAC9 and miR-17 regulates osteogenesis of human periodontal ligament stem cells in inflammatory conditions
    Authors: L Li, W Liu, H Wang, Q Yang, L Zhang, F Jin, Y Jin
    Cell Death Dis, 2018;9(5):480.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Histone methyltransferase G9a drives chemotherapy resistance by regulating the glutamate-cysteine ligase catalytic subunit in head and neck squamous cell carcinoma
    Authors: CW Liu, KT Hua, KC Li, HF Kao, RL Hong, JY Ko, M Hsiao, ML Kuo, CT Tan
    Mol. Cancer Ther, 2017;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  6. Methyltransferase G9a promotes cervical cancer angiogenesis and decreases patient survival
    Authors: RJ Chen, CT Shun, ML Yen, CH Chou, MC Lin
    Oncotarget, 2017;8(37):62081-62098.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  7. The methyltransferase G9a regulates HoxA9-dependent transcription in AML.
    Authors: Lehnertz B, Pabst C, Su L, Miller M, Liu F, Yi L, Zhang R, Krosl J, Yung E, Kirschner J, Rosten P, Underhill T, Jin J, Hebert J, Sauvageau G, Humphries R, Rossi F
    Genes Dev, 2014;28(4):317-27.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Inhibition of G9a induces DUSP4-dependent autophagic cell death in head and neck squamous cell carcinoma.
    Authors: Li, Kai-Chun, Hua, Kuo-Tai, Lin, Yi-Shen, Su, Chia-Yi, Ko, Jenq-Yuh, Hsiao, Michael, Kuo, Min-Lian, Tan, Ching-Ti
    Mol Cancer, 2014;13(0):172.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  9. H3K9 histone methyltransferase G9a promotes lung cancer invasion and metastasis by silencing the cell adhesion molecule Ep-CAM.
    Authors: Chen MW, Hua KT, Kao HJ, Chi CC, Wei LH, Johansson G, Shiah SG, Chen PS, Jeng YM, Cheng TY, Lai TC, Chang JS, Jan YH, Chien MH, Yang CJ, Huang MS, Hsiao M, Kuo ML
    Cancer Res., 2010;70(20):7830-40.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  10. DNA methylation in ES cells requires the lysine methyltransferase G9a but not its catalytic activity.
    Authors: Dong K, Maksakova I, Mohn F, Leung D, Appanah R, Lee S, Yang H, Lam L, Mager D, Schubeler D, Tachibana M, Shinkai Y, Lorincz M
    EMBO J, 0;27(20):2691-701.
    Species: Mouse
    Sample Types: Protein
    Applications: Western Blot


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