Human/Mouse G9a/EHMT2 Antibody
Summary
Preparation and Storage
Reconstitution Calculator
Background
Product Datasheets
Related Research Areas
Human/Mouse G9a/EHMT2 Antibody Summary
Species Reactivity
Human, Mouse
Specificity
This antibody specifically recognizes mouse G9a and cross-reacts with human G9a. Not yet tested in other species.
Source
Monoclonal Mouse IgG2A Clone # A8620A
Purification
Protein A or G purified from ascites
Immunogen
Recombinant mouse G9a/EHMT2
aa 15-114
aa 15-114
Formulation
A liquid formulation in physiologic saline with 0.1% NaN3.
Label
Unconjugated
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Applications
Recommended Concentration
Sample
Western Blot
This antibody can be used at 0.5 μg/mL with the appropriate secondary reagents to detect mouse G9a.
Immunohistochemistry
This antibody can be used at 5 μg/mL with the appropriate secondary reagents to detect mouse G9a.
Immunoprecipitation
Optimal dilutions should be determined by each laboratory.
Direct ELISA
This antibody can be used at 0.1 μg/mL with the appropriate secondary reagents to detect mouse G9a.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Reconstitution Calculator
Preparation and Storage
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
This antibody is stable for greater than six months when held at -20° C in a manual defrost freezer or at -70°C. Upon thawing, the antibody can be stored at 2° - 8° C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Background: G9a/EHMT2
Histone H3 Lys 9 (H3-K9) methylation is a crucial epigenetic marker for transcriptional silencing. G9a is the major mammalian H3-K9 methyltransferase that targets euchromatic regions and is essential for mouse embryogenesis.
Long Name
G9a Histone Methyltransferase/Euchromatic Histone-lysine N-methyltransferase 2
Entrez Gene IDs
10919 (Human); 110147 (Mouse)
Alternate Names
ankyrin repeat-containing protein; BAT8; C6orf30; chromosome 6 open reading frame 30; DKFZp686H08213; EC 2.1.1.-; EC 2.1.1.43; EHMT2; euchromatic histone-lysine N-methyltransferase 2HLA-B-associated transcript 8; FLJ35547; G9A histone methyltransferase; G9a; G9AEm:AF134726.3; H3-K9-HMTase 3; Histone H3-K9 methyltransferase 3; histone-lysine N-methyltransferase, H3 lysine-9 specific 3; HLA-B associated transcript 8; KMT1CNG36/G9a; Lysine N-methyltransferase 1C; NG36; Protein G9a
Product Datasheets
Citations for Human/Mouse G9a/EHMT2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
10
Citations: Showing 1 - 10
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Histone Methyltransferase G9a-Promoted Progression of Hepatocellular Carcinoma Is Targeted by Liver-Specific Hsa-miR-122
Authors: LT Yuan, WJ Lee, YC Yang, BR Chen, CY Yang, MW Chen, JQ Chen, M Hsiao, MH Chien, KT Hua
Cancers, 2021;13(10):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Inhibition of histone methyltransferase G9a attenuates liver cancer initiation by sensitizing DNA-damaged hepatocytes to p53-induced apoptosis
Authors: T Nakatsuka, K Tateishi, H Kato, H Fujiwara, K Yamamoto, Y Kudo, H Nakagawa, Y Tanaka, H Ijichi, T Ikenoue, T Ishizawa, K Hasegawa, M Tachibana, Y Shinkai, K Koike
Cell Death & Disease, 2021;12(1):99.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Cyclin D1 integrates G9a-mediated histone methylation
Authors: Z Li, X Jiao, G Di Sante, A Ertel, MC Casimiro, M Wang, S Katiyar, X Ju, DV Klopfenste, A Tozeren, W Dampier, I Chepelev, A Jeltsch, RG Pestell
Oncogene, 2019;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: ChIP -
Mutual inhibition between HDAC9 and miR-17 regulates osteogenesis of human periodontal ligament stem cells in inflammatory conditions
Authors: L Li, W Liu, H Wang, Q Yang, L Zhang, F Jin, Y Jin
Cell Death Dis, 2018;9(5):480.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Histone methyltransferase G9a drives chemotherapy resistance by regulating the glutamate-cysteine ligase catalytic subunit in head and neck squamous cell carcinoma
Authors: CW Liu, KT Hua, KC Li, HF Kao, RL Hong, JY Ko, M Hsiao, ML Kuo, CT Tan
Mol. Cancer Ther, 2017;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Methyltransferase G9a promotes cervical cancer angiogenesis and decreases patient survival
Authors: RJ Chen, CT Shun, ML Yen, CH Chou, MC Lin
Oncotarget, 2017;8(37):62081-62098.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
The methyltransferase G9a regulates HoxA9-dependent transcription in AML.
Authors: Lehnertz B, Pabst C, Su L, Miller M, Liu F, Yi L, Zhang R, Krosl J, Yung E, Kirschner J, Rosten P, Underhill T, Jin J, Hebert J, Sauvageau G, Humphries R, Rossi F
Genes Dev, 2014;28(4):317-27.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Inhibition of G9a induces DUSP4-dependent autophagic cell death in head and neck squamous cell carcinoma.
Authors: Li, Kai-Chun, Hua, Kuo-Tai, Lin, Yi-Shen, Su, Chia-Yi, Ko, Jenq-Yuh, Hsiao, Michael, Kuo, Min-Lian, Tan, Ching-Ti
Mol Cancer, 2014;13(0):172.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
H3K9 histone methyltransferase G9a promotes lung cancer invasion and metastasis by silencing the cell adhesion molecule Ep-CAM.
Authors: Chen MW, Hua KT, Kao HJ, Chi CC, Wei LH, Johansson G, Shiah SG, Chen PS, Jeng YM, Cheng TY, Lai TC, Chang JS, Jan YH, Chien MH, Yang CJ, Huang MS, Hsiao M, Kuo ML
Cancer Res., 2010;70(20):7830-40.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
DNA methylation in ES cells requires the lysine methyltransferase G9a but not its catalytic activity.
Authors: Dong K, Maksakova I, Mohn F, Leung D, Appanah R, Lee S, Yang H, Lam L, Mager D, Schubeler D, Tachibana M, Shinkai Y, Lorincz M
EMBO J, 0;27(20):2691-701.
Species: Mouse
Sample Types: Protein
Applications: Western Blot
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