Human/Mouse Haptoglobin Antibody

Catalog # Availability Size / Price Qty
AF8465-100
AF8465-SP
Detection  of Human and Mouse Haptoglobin by Western Blot.
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Human/Mouse Haptoglobin Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Haptoglobin in Western blots. In sandwich immunoassays, human Haptoglobin is detected.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Human Haptoglobin from human serum
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Simple Western
1 µg/mL
See below
ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Haptoglobin Monoclonal Antibody (Catalog # MAB8465).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Haptoglobin DuoSet ELISA Kit (Catalog # DY8465-05) for convenient development of a sandwich ELISA or the Human Haptoglobin Quantikine ELISA Kit (Catalog # DHAPG0) for a complete optimized ELISA.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human and Mouse Haptoglobin by Western Blot. View Larger

Detection of Human and Mouse Haptoglobin by Western Blot. Western blot shows human plasma. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse Haptoglobin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8465) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Haptoglobin at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection  of Human and Mouse Haptoglobin by Western Blot. View Larger

Detection of Human and Mouse Haptoglobin by Western Blot. Western blot shows lysates of human Haptoglobin purified from human serum and mouse haptoglobin purified from mouse serum. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse Haptoglobin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8465) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Haptoglobin at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection  of Human Haptoglobin by Simple WesternTM. View Larger

Detection of Human Haptoglobin by Simple WesternTM. Simple Western lane view shows human serum, loaded at 1:25000 dilution. A specific band was detected for Haptoglobin at approximately 60 kDa (as indicated) using 1 µg/mL of Goat Anti-Human/Mouse Haptoglobin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8465) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Human Haptoglobin ELISA Standard Curve. Recombinant Human Haptoglobin protein was serially diluted 2-fold and captured by Mouse Anti-Human Haptoglobin Monoclonal Antibody (Catalog # MAB8465) coated on a Clear Polystyrene Microplate (Catalog # DY990).  Goat Anti-Human/Mouse Haptoglobin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8465) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Haptoglobin

Haptoglobin is a 406 aminoacids (aa) protein that in humans is encoded by the HP gene. This gene encodes a preproprotein that is processed into alpha (aa 19-160) and beta (aa 162-406) chains that combine as a tetramer to produce haptoglobin. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage.

Entrez Gene IDs
3240 (Human); 15439 (Mouse); 24464 (Rat)
Alternate Names
binding peptide; BP; haptoglobin alpha(1S)-beta; haptoglobin alpha(2FS)-beta; Haptoglobin; haptoglobin, alpha polypeptide; haptoglobin, beta polypeptide; HP; HP-1; hp2-alpha; HP2ALPHA2; HPA1S; MGC111141; zonulin

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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