Human Haptoglobin Antibody

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Human Haptoglobin ELISA Standard Curve.
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Product Details
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Human Haptoglobin Antibody Summary

Species Reactivity
Detects human Haptoglobin in direct ELISAs.
Monoclonal Mouse IgG2B Clone # 448018
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human Haptoglobin
Accession # P00738
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration

This antibody functions as an ELISA capture antibody when paired with Goat Anti-Human/Mouse Haptoglobin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8465).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Haptoglobin DuoSet ELISA Kit (Catalog # DY8465-05) for convenient development of a sandwich ELISA or the Human Haptoglobin Quantikine ELISA Kit (Catalog # DHAPG0) for a complete optimized ELISA.


Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Human Haptoglobin ELISA Standard Curve. Recombinant Human Haptoglobin protein was serially diluted 2-fold and captured by Mouse Anti-Human Haptoglobin Monoclonal Antibody (Catalog # MAB8465) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse Haptoglobin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8465) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Haptoglobin

Haptoglobin is a 406 aminoacids (aa) protein that in humans is encoded by the HP gene. This gene encodes a preproprotein that is processed into alpha (aa 19-160) and beta (aa 162-406) chains that combine as a tetramer to produce haptoglobin. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage.

Entrez Gene IDs
3240 (Human); 15439 (Mouse); 24464 (Rat)
Alternate Names
binding peptide; BP; haptoglobin alpha(1S)-beta; haptoglobin alpha(2FS)-beta; Haptoglobin; haptoglobin, alpha polypeptide; haptoglobin, beta polypeptide; HP; HP-1; hp2-alpha; HP2ALPHA2; HPA1S; MGC111141; zonulin

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