Human/Mouse Pin1 Antibody

Catalog # Availability Size / Price Qty
MAB2294
MAB2294-SP
Human/Mouse Pin1 Antibody in Data
3 Images
Product Details
Citations (6)
FAQs
Supplemental Products
Reviews (1)

Human/Mouse Pin1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Pin1 in Western blots.
Source
Monoclonal Mouse IgG2B Clone # 257417
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Pin1
Ala2-Glu163
Accession # Q13526
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below
Immunoprecipitation
2-3 µg/500 µg cell lysate
MCF‑7 human breast cancer cell line and HeLa human cervical epithelial carcinoma cell line, see our available Western blot detection antibodies
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human/Mouse Pin1 antibody by Western Blot. View Larger

Detection of Human/Mouse Pin1 by Western Blot. Western blot shows lysates of Balb/3T3 mouse embryonic fibroblast cell line, U2OS human osteosarcoma cell line, HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, and MDA-MB-453 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse Pin1 Monoclonal Antibody (Catalog # MAB2294) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Pin1 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Pin1 antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC). View Larger

Pin1 in MCF-7 Human Cell Line. Pin1 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human/Mouse Pin1 Monoclonal Antibody (Catalog # MAB2294) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry Pin1 antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

Pin1 in Human Breast Cancer Tissue. Pin1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human/Mouse Pin1 Monoclonal Antibody (Catalog # MAB2294) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pin1

Pin1 is a peptidyl-prolyl isomerase (PPI) that targets phosphorylated Ser or Thr residues followed by a Pro (S/T-P). Isomerization of phosphorylated Ser or Thr residues may alter protein confirmation and, subsequently, modify activity. Pin1 is overexpressed in many human breast cancers, and has been shown to modify numerous proteins including p53,NF-kappa B, c-Jun, cyclin D1, and beta -catenin.

Long Name
Protein [Peptidyl-prolyl cis/trans Isomerase] NIMA-interacting 1
Entrez Gene IDs
5300 (Human)
Alternate Names
dod; EC 5.2.1.8; peptidylprolyl cis/trans isomerase, NIMA-interacting 1; peptidyl-prolyl cis/trans isomerase, NIMA-interacting; peptidyl-prolyl cis-trans isomerase NIMA-interacting 1; Peptidyl-prolyl cis-trans isomerase Pin1; Pin1; PPIase Pin1; prolyl isomerase; protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1; protein (peptidylprolyl cis/trans isomerase) NIMA-interacting 1; Rotamase Pin1; UBL5

Product Datasheets

Citations for Human/Mouse Pin1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. TLR-7 Stress Signaling in Differentiating and Mature Eosinophils Is Mediated by the Prolyl Isomerase Pin1
    Authors: ZJ Shen, J Hu, V Kashi, YA Bochkov, JE Gern, JS Malter
    J. Immunol., 2018;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  2. The peptidyl-prolyl isomerase Pin1 up-regulation and proapoptotic function in dopaminergic neurons: relevance to the pathogenesis of Parkinson disease.
    Authors: Ghosh A, Saminathan H, Kanthasamy A, Anantharam V, Jin H, Sondarva G, Harischandra D, Qian Z, Rana A, Kanthasamy A
    J Biol Chem, 2013;288(30):21955-71.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. A distinct role for Pin1 in the induction and maintenance of pluripotency.
    Authors: Nishi M, Akutsu H, Masui S
    J. Biol. Chem., 2011;286(13):11593-603.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  4. The peptidyl-isomerase Pin1 regulates p27kip1 expression through inhibition of Forkhead box O tumor suppressors.
    Authors: Brenkman AB, de Keizer PL, van den Broek NJ, van der Groep P, van Diest PJ, van der Horst A, Smits AM, Burgering BM
    Cancer Res., 2008;68(18):7597-605.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Protein Never in Mitosis Gene A Interacting-1 (PIN1) regulates degradation of inducible nitric oxide synthase in endothelial cells.
    Authors: Liu T, Huang Y, Likhotvorik RI, Keshvara L, Hoyt DG
    Am. J. Physiol., Cell Physiol., 2008;295(3):C819-27.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  6. Pin1 regulates TGF-beta1 production by activated human and murine eosinophils and contributes to allergic lung fibrosis.
    Authors: Shen ZJ, Esnault S, Rosenthal LA, Szakaly RJ, Sorkness RL, Westmark PR, Sandor M, Malter JS
    J. Clin. Invest., 2008;118(2):479-90.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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Reviews for Human/Mouse Pin1 Antibody

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Human/Mouse Pin1 Antibody
By Anonymous on 12/07/2020
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma