Human/Mouse/Rat alpha Tubulin Antibody Summary
Accession # Q71U36
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human, Mouse, and Rat
alpha Tubulin by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, NIH‑3T3 mouse embryonic fibroblast cell line,
Neuro‑2A mouse neuroblastoma cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.05 µg/mL of Mouse Anti-Human/Mouse/Rat alpha Tubulin Monoclonal Antibody (Catalog # MAB9344) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for alpha Tubulin at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
alpha Tubulin in SH‑SY5Y Human Cell Line.
alpha Tubulin was detected in immersion fixed
SH‑SY5Y human neuroblastoma cell line using Mouse Anti-Human/Mouse/Rat alpha Tubulin Monoclonal Antibody (Catalog # MAB9344) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
alpha Tubulin in Human Brain. alpha Tubulin was detected in immersion fixed paraffin-embedded sections of human brain using Mouse Anti-Human/Mouse/Rat alpha Tubulin Monoclonal Antibody (Catalog # MAB9344) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha Tubulin
Tubulin alpha-1A chain (TUBA1A), also known as Alpha-tubulin 3, is the principal alpha tubulin in morphologically differentiated neurons. Alpha tubulin dimerizes with beta Tubulin to form microtubules. Microtubules mediate transport of proteins and endosomes within cells, and TUBA1A has been shown to interact with metabotropic glutamate receptor 7 (receptor trafficking), synuclein alpha (synaptic plasticity), and N-syndecan (neurite outgrowth). This gene is mutated in malformations of cortical development, including Lissencephaly resulting in microcephaly, developmental delay and early-onset epileptic seizures.
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