Human/Mouse/Rat alpha Tubulin Antibody

Catalog # Availability Size / Price Qty
MAB9344-100
MAB9344-SP
Detection  of Human, Mouse, and Rat  alpha  Tubulin by Western Blot.
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Human/Mouse/Rat alpha Tubulin Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human alpha Tubulin in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2A Clone # 961216
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human TUBA1A synthetic peptide
Accession # Q71U36
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.05 µg/mL
See below
Immunohistochemistry
5-25 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human, Mouse, and Rat  alpha  Tubulin by Western Blot. View Larger

Detection of Human, Mouse, and Rat
alpha Tubulin by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, NIH‑3T3 mouse embryonic fibroblast cell line,
Neuro‑2A mouse neuroblastoma cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.05 µg/mL of Mouse Anti-Human/Mouse/Rat alpha Tubulin Monoclonal Antibody (Catalog # MAB9344) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for alpha Tubulin at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry alpha   Tubulin in SH‑SY5Y Human Cell Line. View Larger

alpha Tubulin in SH‑SY5Y Human Cell Line.
alpha Tubulin was detected in immersion fixed
SH‑SY5Y human neuroblastoma cell line using Mouse Anti-Human/Mouse/Rat alpha Tubulin Monoclonal Antibody (Catalog # MAB9344) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry alpha   Tubulin in Human Brain. View Larger

alpha Tubulin in Human Brain. alpha Tubulin was detected in immersion fixed paraffin-embedded sections of human brain using Mouse Anti-Human/Mouse/Rat alpha Tubulin Monoclonal Antibody (Catalog # MAB9344) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha Tubulin

Tubulin alpha-1A chain (TUBA1A), also known as Alpha-tubulin 3, is the principal alpha tubulin in morphologically differentiated neurons. Alpha tubulin dimerizes with beta Tubulin to form microtubules. Microtubules mediate transport of proteins and endosomes within cells, and TUBA1A has been shown to interact with metabotropic glutamate receptor 7 (receptor trafficking), synuclein alpha (synaptic plasticity), and N-syndecan (neurite outgrowth).  This gene is mutated in malformations of cortical development, including Lissencephaly resulting in microcephaly, developmental delay and early-onset epileptic seizures.

Long Name
Tubulin Alpha 1a
Entrez Gene IDs
7846 (Human); 22142 (Mouse); 64158 (Rat)
Alternate Names
alpha Tubulin; Alpha-Tubulin 3; B-ALPHA-1; FLJ25113; hum-a-tub2; LIS3; TUBA1A; TUBA3; TUBA3hum-a-tub1; tubulin alpha-1A chain; Tubulin alpha-3 chain; Tubulin B-Alpha-1; tubulin, alpha 1a; tubulin, alpha 3; tubulin, alpha, brain-specific

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Mouse IgG2A Isotype Control

Ctrl

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2A Antibody

Mouse IgG2A Antibody

Reviews for Human/Mouse/Rat alpha Tubulin Antibody

Average Rating: 5 (Based on 2 Reviews)

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Human/Mouse/Rat alpha Tubulin Antibody
By Anonymous on 07/21/2018
Application: WB Sample Tested: HEK293T human embryonic kidney cell line Species: Human

Human/Mouse/Rat alpha Tubulin Antibody
By Anonymous on 07/17/2018
Application: WB Sample Tested: HEK293 human embryonic kidney cell line Species: Human