alpha Tubulin Antibody (961216)

R&D Systems | Catalog # MAB9344

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Primate - Cercopithecus aethiops (African Green Monkey), Primate - Chlorocebus aethiops (African Green Monkey)

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Western Blot, Westen Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 961216
View all alpha Tubulin Primary Antibodies »

Product Specifications

Immunogen

Human TUBA1A synthetic peptide
Accession # Q71U36

Specificity

Detects human alpha Tubulin in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for alpha Tubulin Antibody (961216)

Detection of Human, Mouse, and Rat a Tubulin antibody by Western Blot.

Detection of Human, Mouse, and Rat alpha Tubulin by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, NIH-3T3 mouse embryonic fibroblast cell line, Neuro-2A mouse neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.05 µg/mL of Mouse Anti-Human/Mouse/Rat a Tubulin Monoclonal Antibody (Catalog # MAB9344) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a Tubulin at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
a Tubulin antibody in SH-SY5Y Human Cell Line by Immunocytochemistry (ICC).

alpha Tubulin in SH‑SY5Y Human Cell Line.

a Tubulin was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Mouse Anti-Human/Mouse/Rat a Tubulin Monoclonal Antibody (Catalog # MAB9344) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
a Tubulin antibody in Human Brain by Immunohistochemistry (IHC-P).

alpha Tubulin in Human Brain.

a Tubulin was detected in immersion fixed paraffin-embedded sections of human brain using Mouse Anti-Human/Mouse/Rat a Tubulin Monoclonal Antibody (Catalog # MAB9344) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of fnwlvcom8wgvefn3cn9c.jpg by Western Blot

Detection of fnwlvcom8wgvefn3cn9c.jpg by Western Blot

Effects of miR-210-KO in M0 macrophages. (A) Enrichr dot-plot representation for pathways enriched among differentially expressed genes in miR-210-KO versus WT M0 macrophages. The signed odds ratio (x-axis) indicates over-representation in up-regulated (positive) or down-regulated (negative) genes. (B) Western blot analysis of pro-IL1 beta levels in M0 macrophages, with alpha -tubulin used as a loading control. Densitometry analysis performed using TotalLab. Data are presented as the mean ± SEM, n = 3 mice per condition. Statistical significance was determined using Student’s t test, *p< 0.05. (C) ELISA quantification of IL-6, TNF-alpha and IL-1 beta in the supernatant of M0 macrophages. Data are presented as mean ± SEM, n = 3 for IL-6 and IL-1 beta and n=4 for TNF-alpha. Image collected and cropped by CiteAb from the following open publication (https://www.frontiersin.org/articles/10.3389/fimmu.2025.1633163/full), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of kbaunvjo34l0yjgizioe.jpg by Western Blot

Detection of kbaunvjo34l0yjgizioe.jpg by Western Blot

Effects of eHSP70 on the expression of MAPKs in NuLi-1 cells. (a) Representative Western blots and bar chart show that exposure to eHSP70 over 24 h did not change the level of AKT (left) or p38 MAPK (right) expression in NuLi-1 cells. Bars show mean ± S.E.M. of three independent experiments. (b) Representative Western blots and subsequent image analysis show that eHSP70 significantly reduced ERK1/2 expression and (c) phosphorylation of ERK1/2 over 24 h in NuLi-1 cells. Bars show mean ± S.E.M. of five different human primary bronchial EC lines. p-values are indicated as follows: * p ≤ 0.05; ** p ≤ 0.01. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37511459), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for alpha Tubulin Antibody (961216)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed SH-SY5Y human neuroblastoma cell line

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain

Western Blot

0.05 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, NIH‑3T3 mouse embryonic fibroblast cell line, Neuro‑2A mouse neuroblastoma cell line, and Rat‑2 rat embryonic fibroblast cell line

Reviewed Applications

Read 4 reviews rated 5 using MAB9344 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: alpha Tubulin

Tubulin alpha-1A chain (TUBA1A), also known as Alpha-tubulin 3, is the principal alpha tubulin in morphologically differentiated neurons. Alpha tubulin dimerizes with beta Tubulin to form microtubules. Microtubules mediate transport of proteins and endosomes within cells, and TUBA1A has been shown to interact with metabotropic glutamate receptor 7 (receptor trafficking), synuclein alpha (synaptic plasticity), and N-syndecan (neurite outgrowth).  This gene is mutated in malformations of cortical development, including Lissencephaly resulting in microcephaly, developmental delay and early-onset epileptic seizures.

Long Name

Tubulin Alpha 1a

Alternate Names

Alpha-Tubulin 3, B-ALPHA-1, LIS3, TUBA1A, TUBA3, Tubulin B-Alpha-1

Entrez Gene IDs

7846 (Human); 22142 (Mouse); 64158 (Rat)

Gene Symbol

TUBA1A

UniProt

Q71U36

Additional alpha Tubulin Products

Product Documents for alpha Tubulin Antibody (961216)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for alpha Tubulin Antibody (961216)

For research use only

Related Research Areas

Microtubules

Citations for alpha Tubulin Antibody (961216)

Customer Reviews for alpha Tubulin Antibody (961216) (4)

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4 Customer Ratings
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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • Human/Mouse/Rat alpha Tubulin Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Human fibroblast
    Species: Human
    Verified Customer | Posted 11/23/2023
    Paraformaldehyde-fixed fibroblast stained with alpha tubulin (red), and counterstained with DAPI
    alpha Tubulin Antibody (961216) MAB9344
  • Human/Mouse/Rat alpha Tubulin Antibody
    Name: Ricardo Malvicini
    Application: Dot Blot
    Sample Tested: Mesenchymal stem cells
    Species: Human
    Verified Customer | Posted 06/23/2023
    different protein quantities of an MSC lysate were used to test the antibody.
    alpha Tubulin Antibody (961216) MAB9344
  • Human/Mouse/Rat alpha Tubulin Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HEK293T human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 07/21/2018
    alpha Tubulin Antibody (961216) MAB9344
  • Human/Mouse/Rat alpha Tubulin Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HEK293 human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 07/17/2018
    alpha Tubulin Antibody (961216) MAB9344

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Protocols

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