Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Western Blot, Immunoprecipitation, Bioassay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all AMPK alpha 1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human AMPK alpha 1
Lys349-Gln559
Accession # Q13131

Specificity

Detects human, mouse, and rat AMPK alpha 1 in Western blots. The antibody does not cross-react with recombinant human AMPK alpha 2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat AMPK alpha 1 Antibody

AMPKa1 antibody in Human Liver by Immunohistochemistry (IHC-P).

AMPK alpha 1 in Human Liver.

AMPKa1 was detected in immersion fixed paraffin-embedded sections of human liver using 5 µg/mL Goat Anti-Human/Mouse/Rat AMPKa1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3197) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows secondary antibody only control experiment. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human. Mouse, and Rat AMPKa1 antibody by Western Blot.

Detection of Human. Mouse, and Rat AMPK alpha 1 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse/Rat AMPKa1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3197) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for AMPKa1 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
AMPKa1 antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC).

AMPK alpha 1 in MCF‑7 Human Cell Line.

AMPKa1 was detected in immersion fixed MCF-7 human breast cancer cell line using Goat Anti-Human/Mouse/Rat AMPKa1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3197) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of AMPK alpha 1 by Western Blot

Detection of AMPK alpha 1 by Western Blot

TSPAN1 knockdown decreases cell proliferation in OCCC cell lines. (A) TSPAN1 expression was detected by real‐time PCR (left panel) and immunoblotting (right panel) in TOV‐21G and OVTOKO cells, with alpha ‐actinin as an internal loading control. Data are expressed as the mean ± standard error (SE); n = 3. Unpaired t‐test was performed. *P < 0.05, **P < 0.01. (B) Cell proliferation was determined via crystal violet staining at various times after transient transfection of siControl or siTSPAN1 in TOV‐21G and OVTOKO cells. Error bars represent mean ± SE; n = 3. Unpaired t‐test was performed. **P < 0.01, ***P < 0.001. (C) Colony forming assay was performed in TOV‐21G and OVTOKO cells. Upper panel shows representative images; lower panel shows the relative absorbance at 595 nm. Error bars represent mean ± SE; n = 3. Unpaired t‐test was performed. **P < 0.01, ***P < 0.001. (D) Protein expressions were detected using immunoblotting after transient transfection of siControl or siTSPAN1 in TOV‐21G and OVTOKO cells, with alpha ‐actinin as an internal loading control. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33331115), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse/Rat AMPK alpha 1 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Western Blot

2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and Rat‑2 rat embryonic fibroblast cell line

Reviewed Applications

Read 2 reviews rated 5 using AF3197 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: AMPK alpha 1

AMP-activated protein kinase (AMPK) is a heterotrimeric complex consisting of a catalytic  alpha subunit and regulatory beta  and gamma  subunits. Each subunit exists as alternate isoforms ( alpha 1,  alpha 2, beta 1,  beta 2,  gamma 1,  gamma 2,  gamma 3), with all 12 combinations able to form complexes. The catalytic alpha  subunit of AMPK is activated allosterically by AMP, and by phosphorylation via the AMPK kinases LKB1 and CaMKK beta. AMPK’s role in metabolic regulation has implicated this serine/threonine kinase as a therapeutic target in heart disease, obesity, and diabetes.

Long Name

AMP-activated Protein Kinase alpha 1

Alternate Names

PRKAA1

Entrez Gene IDs

5562 (Human)

Gene Symbol

PRKAA1

UniProt

Q13131

Additional AMPK alpha 1 Products

Product Documents for Human/Mouse/Rat AMPK alpha 1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat AMPK alpha 1 Antibody

For research use only

Related Research Areas

Autophagy
Intracellular Kinases
Obesity

Citations for Human/Mouse/Rat AMPK alpha 1 Antibody

Customer Reviews for Human/Mouse/Rat AMPK alpha 1 Antibody (2)

5 out of 5
2 Customer Ratings
5 Stars
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4 Stars
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3 Stars
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1 Stars
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  • Human/Mouse/Rat AMPK alpha 1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Endothelial
    Species: Mouse
    Verified Customer | Posted 10/11/2019
    Human/Mouse/Rat AMPK alpha 1 Antibody AF3197
  • Human/Mouse/Rat AMPK alpha 1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: MDA-MB-231 human breast cancer cell line
    Species: Human
    Verified Customer | Posted 10/17/2018
    Human/Mouse/Rat AMPK alpha 1 Antibody AF3197

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