AMPK alpha 1 in Human Liver.
AMPK alpha 1 was detected in immersion fixed paraffin-embedded sections of human liver using 5 µg/mL Goat Anti-Human/Mouse/Rat AMPK alpha 1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3197) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP‑DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows secondary antibody only control experiment. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human/Mouse/Rat AMPK alpha 1 by Western Blot.
Western blot shows lysates of MCF‑7 human breast cancer cell line, PT18 mouse mast/basophil cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat AMPK alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3197) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for AMPK alpha 1 at approximately 63 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
AMPK alpha 1 in MCF‑7 Human Cell Line.
AMPK alpha 1 was detected in immersion fixed MCF‑7 human breast cancer cell line using Goat Anti-Human/Mouse/Rat AMPK alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3197) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: AMPK alpha 1
AMP-activated protein kinase (AMPK) is a heterotrimeric complex consisting of a catalytic alpha subunit and regulatory beta and gamma subunits. Each subunit exists as alternate isoforms ( alpha 1, alpha 2, beta 1, beta 2, gamma 1, gamma 2, gamma 3), with all 12 combinations able to form complexes. The catalytic alpha subunit of AMPK is activated allosterically by AMP, and by phosphorylation via the AMPK kinases LKB1 and CaMKK beta. AMPK’s role in metabolic regulation has implicated this serine/threonine kinase as a therapeutic target in heart disease, obesity, and diabetes.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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