Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry, Simple Western

Cited:

Immunohistochemistry, Western Blot, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all AMPK alpha 2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human AMPK alpha 2
Phe340-Arg552
Accession # P54646

Specificity

Detects human, mouse, and rat AMPK alpha 2 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human AMPK alpha 1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat AMPK alpha 2 Antibody

Detection of Human and Mouse AMPKa2 antibody by Western Blot.

Detection of Human and Mouse AMPK alpha 2 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and mouse liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat AMPKa2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2850) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for AMPKa2 at approximately 63 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human AMPKa2 antibody by Simple WesternTM.

Detection of Human AMPK alpha 2 by Simple WesternTM.

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for AMPKa2 at approximately 63 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat AMPKa2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2850) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

AMPKa2 antibody in HEK293 Human Cell Line by Immunocytochemistry (ICC).

AMPK alpha 2 in HEK293 Human Cell Line.

AMPKa2 was detected in immersion fixed HEK293 human embryonic kidney cell line using Goat Anti-Human/Mouse/Rat AMPKa2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2850) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human/Mouse/Rat AMPK alpha 2 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HEK293 human embryonic kidney cell line

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse liver

Simple Western

10 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and mouse liver tissue

Reviewed Applications

Read 1 review rated 3 using AF2850 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: AMPK alpha 2

AMP-activated protein kinase (AMPK) is a heterotrimeric complex consisting of a catalytic alpha subunit and regulatory beta and gamma subunits. Each subunit exists as alternate isoforms ( alpha 1, alpha 2, beta 1, beta 2, gamma 1, gamma 2, gamma 3), with all 12 combinations able to form complexes. The catalytic alpha subunit of AMPK is activated allosterically by AMP, and by phosphorylation via the AMPK kinase LKB1. AMPK’s role in metabolic regulation has implicated this serine/threonine kinase as a therapeutic target in heart disease, obesity, and diabetes.

Long Name

AMP-activated Protein Kinase alpha

Alternate Names

PRKAA2

Entrez Gene IDs

5563 (Human); 108079 (Mouse); 78975 (Rat)

Gene Symbol

PRKAA2

UniProt

P54646

Additional AMPK alpha 2 Products

Product Documents for Human/Mouse/Rat AMPK alpha 2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat AMPK alpha 2 Antibody

For research use only

Related Research Areas

Autophagy
Intracellular Kinases
Obesity

Citations for Human/Mouse/Rat AMPK alpha 2 Antibody

Customer Reviews for Human/Mouse/Rat AMPK alpha 2 Antibody (1)

3 out of 5
1 Customer Rating
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  • Human/Mouse/Rat AMPK alpha 2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Adipose tissue
    Species: Mouse
    Verified Customer | Posted 07/19/2019
    Human/Mouse/Rat AMPK alpha 2 Antibody AF2850

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Protocols

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