Human/Mouse/Rat Beclin 1 Antibody

Catalog # Availability Size / Price Qty
MAB5295
MAB5295-SP
Detection of Human, Mouse, and Rat Beclin 1 by Western Blot.
4 Images
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (1)

Human/Mouse/Rat Beclin 1 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human Beclin 1 in direct ELISAs. Detects human, mouse, and rat Beclin 1 in Western blots. In direct ELISAs,
no cross-reactivity with recombinant human ATG3, 4A, 4B, 5, 7, 10, 12, or 16L1 is observed.
Source
Monoclonal Mouse IgG2B Clone # 669922
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E.coli-derived recombinant human Beclin 1
Met12-Gln239
Accession # Q14457
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Simple Western
1 µg/mL
See below
Immunohistochemistry
3-15 µg/mL
Immersion fixed paraffin-embedded sections of Human Kidney

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat Beclin 1 antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat Beclin 1 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, A20 mouse B cell lymphoma cell line, and NRK rat normal kidney cell line. PVDF Membrane was probed with 0.1 µg/mL of Mouse Anti-Human Beclin 1 Monoclonal Antibody (Catalog # MAB5295) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for Beclin 1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Simple Western Detection of Human Beclin 1 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human Beclin 1 by Simple WesternTM. Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for Beclin 1 at approximately 58 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human/Mouse/Rat Beclin 1 Monoclonal Antibody (Catalog # MAB5295). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Simple Western Detection of Mouse Beclin 1 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Mouse Beclin 1 by Simple WesternTM. Simple Western lane view shows lysates of mouse brain tissue, loaded at 0.2 mg/mL. A specific band was detected for Beclin 1 at approximately 58 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human/Mouse/Rat Beclin 1 Monoclonal Antibody (Catalog # MAB5295). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunohistochemistry View Larger

Detection of Beclin 1 in Human Kidney. Beclin 1 was detected in immersion fixed paraffin-embedded sections of Human Kidney using Mouse Anti-Human/Mouse/Rat Beclin 1 Monoclonal Antibody (Catalog # MAB5295) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to glomeruli. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Beclin 1

Beclin 1 (Bcl-2 interacting coiled-coil protein; also know as ATG6) is a 60 kDa member of the Beclin family of proteins. It is part of a lipid-kinase complex that includes UVRAG and PI3 and induces autophagy; in the presence of Bcl-2, autophagy is repressed. Beclin 1 is widely expressed but abundantly found in mammary epithelium, glia and neurons. Human Beclin 1 is 450 amino acids (aa) in length and contains a Bcl-2 binding domain (aa 88‑150), a nuclear export signal motif (aa 180‑189), a coiled-coil region (aa 142‑270), and an evolutionary conserved domain (aa 244‑337). There is one potential alternate start site at Met88. Over aa 12‑239, human Beclin 1 is 98% aa identical to mouse Beclin 1.

Long Name
Beclin1, Autophagy-related
Entrez Gene IDs
8678 (Human); 56208 (Mouse); 114558 (Rat)
Alternate Names
ATG6 autophagy related 6 homolog; ATG6; beclin 1 (coiled-coil, moesin-like BCL2 interacting protein); beclin 1 (coiled-coil, moesin-like BCL2-interacting protein); Beclin 1; beclin 1, autophagy related; beclin1; beclin-1; BECN1; Coiled-coil myosin-like BCL2-interacting protein; GT197; Protein GT197; VPS30

Product Datasheets

You must select a language.

x

Citations for Human/Mouse/Rat Beclin 1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. Calcitriol Inhibits Proliferation and Potentially Induces Apoptosis in B16-F10 Cells
    Authors: Eva Krishna Sutedja, Daniar Amarassaphira, Hanna Goenawan, Yuni Susanti Susanti Pratiwi, Nova Sylviana, Budi Setiabudiawan et al.
    Medical Science Monitor Basic Research
  2. GK-1 Induces Oxidative Stress, Mitochondrial Dysfunction, Decreased Membrane Potential, and Impaired Autophagy Flux in a Mouse Model of Breast Cancer
    Authors: Alfredo Cruz-Gregorio, Ana Karina Aranda-Rivera, Omar Emiliano Aparicio-Trejo, Omar Noel Medina-Campos, Edda Sciutto, Gladis Fragoso et al.
    Antioxidants (Basel)
  3. Bupivacaine at clinically relevant concentrations induces toxicity in human intervertebral disc cells via the induction of autophagy in vitro
    Authors: G Yang, Z Li, H Mei, W Ye, S Huang, K Liu, Q Tan
    Mol Med Rep, 2019-05-22;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human/Mouse/Rat Beclin 1 Antibody

Average Rating: 5 (Based on 1 Review)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human/Mouse/Rat Beclin 1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human/Mouse/Rat Beclin 1 Antibody
By Anonymous on 10/17/2018
Application: WB Sample Tested: MDA-MB-231 human breast cancer cell line Species: Human