beta-Catenin Antibody

R&D Systems | Catalog # AF1329

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat, Avian - Chicken, Hamster, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Intracellular Staining by Flow Cytometry, Immunocytochemistry, Simple Western, Chromatin Immunoprecipitation (ChIP), CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation Sequencing, Bioassay, Functional Assay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all beta-Catenin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human beta -Catenin
Ala2-Leu781
Accession # P35222

Specificity

Detects human, mouse and rat beta -Catenin in direct ELISAs.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for beta-Catenin Antibody

Detection of Human/Mouse/Rat beta ‑Catenin antibody by Western Blot.

Detection of Human/Mouse/Rat beta ‑Catenin by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, C6 rat glioma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse/Rat beta -Catenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1329) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). For additional reference, recombinant human beta -catenin (1 ng) was included. A specific band for beta-Catenin was detected at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of beta -Catenin-regul-ated Genes antibody by Chromatin Immunoprecipitation.

Detection of beta ‑Catenin-regul-ated Genes by Chromatin Immunoprecipitation.

HeLa human cervical epithelial carcinoma cell line were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. beta -Catenin/ DNA complexes were immuno-precipitated using 5 µg Goat Anti-Human/Mouse/Rat beta -Catenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1329) or control antibody (AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immuno-complexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. TheSU(Z)12promoter was detected by standard PCR.
beta -Catenin antibody in SW480 Human Cell Line by Immunocytochemistry (ICC).

beta ‑Catenin in SW480 Human Cell Line.

beta -Catenin was detected in immersion fixed SW480 human colorectal adenocarcinoma cell line using Goat Anti-Human/Mouse/Rat beta -Catenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1329) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
beta -Catenin antibody in Human Kidney Cancer Tissue by Immunohistochemistry (IHC-P).

beta -Catenin in Human Kidney Cancer Tissue.

beta -Catenin was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human/Mouse/Rat beta -Catenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1329) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
beta -Catenin antibody in Human Kidney Cancer Tissue by Immunohistochemistry (IHC-P).

beta ‑Catenin in Human Kidney Cancer Tissue.

beta -Catenin was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using 15 µg/mL Goat Anti-Human/Mouse/Rat beta -Catenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1329) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to epithelial cells in collecting tubules in the medulla. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of beta -Catenin antibody in HeLa Human Cell Line antibody by Flow Cytometry.

Detection of beta ‑Catenin in HeLa Human Cell Line by Flow Cytometry.

HeLa human cervical epithelial carcinoma cell line was stained with Goat Anti-Human/Mouse/Rat beta -Catenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1329, filled histogram) or control antibody (AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Detection of Human and Mouse beta ‑Catenin antibody by Simple WesternTM.

Detection of Human and Mouse beta ‑Catenin by Simple WesternTM.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH-3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for beta -Catenin at approximately 94-97 kDa (as indicated) using 50 µg/mL of Goat Anti-Human/Mouse/Rat beta -Catenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1329) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Detection of Human beta-Catenin by Western Blot

Detection of Human beta-Catenin by Western Blot

The effects of montelukast on ROS generation and of the inhibition of ROS generation on arsenic-induced EMT of NHBE cells. (A) Cells seeded in 96-well plates were pretreated with montelukast at 0.1 and 1 µM for 2 h, and then 1 µM NaAsO2 was applied for combination treatment. After 3 h of treatment, the cells were stained with 10 µM DCFH-DA for 30 min. After washing out the residue dye, the fluorescent signal was measured immediately. The quantitative results (left panel) and representative images (right panel) are shown. a: p < 0.05 compared with the vehicle control group. b: p < 0.05 compared with the NaAsO2 group. (B) Cells were pretreated with NAC for 2 h, and then 1 µM NaAsO2 for another 24 h. A wound was made with a 200-µl tip, and the wound coverage was analyzed 6 h after the wound was made. a: p < 0.05 compared with the vehicle control group. b: p < 0.05 compared with the NaAsO2 group. (C) Cells were pretreated with NAC for 2 h, and then 1 µM NaAsO2 was added for another 24 h. The cells were harvested for protein expression analysis by western blotting. a: p < 0.05 compared with the vehicle control group. b: p < 0.05 compared with the NaAsO2 group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35517780), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human beta-Catenin by Western Blot

Detection of Human beta-Catenin by Western Blot

The effect of montelukast on arsenic-induced EMT marker expression in NHBE cells. Cells were pretreated with montelukast for 2 h, followed by combined treatment with 1 µM NaAsO2. After 24 h of treatment, the cells were harvested for protein expression analysis by western blotting. (A) Representative western blot images. (B–G) The quantitative results of protein expression changes are shown in (C–G). a: p < 0.05 compared with the vehicle control group. b: p < 0.05 compared with the NaAsO2 group. (H) The cell morphology changes. NHBE cells were pretreated with 0.1 or 1 µM montelukast respectively for 2 h and followed by sodium arsenite for another 48 h. The cell morphology was analyzed by radius-ratio methods. a: p < 0.05 compared with the vehicle control group. b: p < 0.05 compared with the NaAsO2 group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35517780), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human beta-Catenin by Western Blot

Detection of Human beta-Catenin by Western Blot

Effect of the combination of montelukast and fluticasone on NaAsO2-induced cell migration of NHBE cells. Cells were pretreated with medications for 2 h and then arsenic was added. (A,B) After 24 h of incubation, the cells were trypsinized, plated onto the inner side of the Transwell chamber and incubated for 36 h. The migrated cells were analyzed by the ratio of the bottom/total of the cell occupied area. Representative images (left panel) and the quantitative results are shown (right panel). (C–I) After 24 of incubation, the protein lysates were extracted and applied to western blot analysis. The representative image was shown in (C), and the quantitative results were shown, respectively (D–I). a: p < 0.05 compared with the vehicle control group. b: p < 0.05 compared with the NaAsO2 group. c: p < 0.05 compared with the montelukast plus NaAsO2 group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35517780), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human beta-Catenin by Western Blot

Detection of Human beta-Catenin by Western Blot

The effects of montelukast on NF-kappa B activation and of the inhibition of NF-kappa B on mediated arsenic-induced EMT of NHBE cells. (A) Arsenic induced the phosphorylation of AKT and p65. Cells were treated with 1 µM NaAsO2 for different time intervals, and the cells were harvested and subjected to western blot analysis using a phospho-antibody. (B) NAC treatment inhibited arsenic-induced phospho-p65. Cells were pretreated with NAC for 2 h, and then NaAsO2 was added for another 3 h. The expression of phospho-p65 was determined with a phospho-Ser536-p65 antibody. GAPDH served as the internal control. (C) Treatment with an NF-kappa B inhibitor reduced arsenic-induced cell migration. Cells were pretreated with 2 µM BAY117082 for 2 h and then treated with 1 µM NaAsO2 for another 24 h. A wound was made with a 200-µl tip, and the wound coverage was analyzed by comparing the cell-occupied area at 0 h versus that 6 h after the wound was made. The representative image (left panel) and the quantitative results (right panel) are shown. a: p < 0.05 compared with the vehicle control group. b: p < 0.05 compared with the NaAsO2 group. (D) Treatment with an NF-kappa B inhibitor reduced arsenic-induced mesenchymal marker expression. Cells were pretreated with 2 µM BAY117082 for 2 h, and then 1 µM NaAsO2 was added for another 24 h. The cells were harvested for protein expression analysis by western blotting. (E) Montelukast inhibited arsenic-induced phospho-p65 expression. Cells were pretreated with montelukast for 2 h, and then arsenic was added for another 3 h. The cells were harvested for protein expression analysis by western blotting. GAPDH served as the internal control. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35517780), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of beta-Catenin by Western Blot

Detection of beta-Catenin by Western Blot

Immunoblotting (a) with densitometry analysis, and (b) of the tdTomato-positive cells isolated from VE-cadherincreERT2RosatdTomatoIns2Akita/+ and VE-cadherincreERT2RosatdTomato mice treated with or without SB216763 (n = 3). The numbers represent each sample. Densitometry was analyzed for statistical significance by ANOVA with post hoc Tukey’s analysis. *** p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36983045), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of beta-Catenin by Western Blot

Detection of beta-Catenin by Western Blot

Immunoblotting (a) with densitometry analysis, and (b) of the tdTomato-positive cells isolated from VE-cadherincreERT2RosatdTomatoIns2Akita/+ and VE-cadherincreERT2RosatdTomato mice treated with or without SB216763 (n = 3). The numbers represent each sample. Densitometry was analyzed for statistical significance by ANOVA with post hoc Tukey’s analysis. *** p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36983045), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for beta-Catenin Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

5 µg/5 x 106 cells
Sample: HeLa human cervical epithelial carcinoma cell line chromatin, SU(Z)12 promoter detected by standard PCR.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed SW480 human colorectal adenocarcinoma cell line

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney cancer tissue

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: HeLa human cervical epithelial carcinoma cell line fixed with paraformaldehyde and permeabilized with saponin

Simple Western

50 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, C6 rat glioma cell line, and NIH-3T3 mouse embryonic fibroblast cell line

Reviewed Applications

Read 6 reviews rated 4.3 using AF1329 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: beta-Catenin

beta -Catenin is a cadherin-associated protein that is involved in the regulation of cell adhesion. It also acts as a transcriptional co-activator in the nucleus and is involved the canonical Wnt signal transduction pathways.

Alternate Names

bCatenin, CTNNB1

Entrez Gene IDs

1499 (Human); 12387 (Mouse); 84353 (Rat)

Gene Symbol

CTNNB1

UniProt

P35222

Additional beta-Catenin Products

Product Documents for beta-Catenin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for beta-Catenin Antibody

For research use only

Citations for beta-Catenin Antibody

Customer Reviews for beta-Catenin Antibody (6)

4.3 out of 5
6 Customer Ratings
5 Stars
33%
4 Stars
67%
3 Stars
0%
2 Stars
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1 Stars
0%

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Customer Images

Showing  1 - 5 of 6 reviews Showing All
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  • Human/Mouse/Rat beta-Catenin Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: epithelium
    Species: Mouse
    Verified Customer | Posted 06/27/2021
    beta-Catenin Antibody AF1329
  • Human/Mouse/Rat beta-Catenin Antibody
    Name: Tina Yuan
    Application: Immunohistochemistry
    Sample Tested: mouse oral epithelium
    Species: Mouse
    Verified Customer | Posted 11/23/2020
    beta-Catenin Antibody AF1329
  • Human/Mouse/Rat beta -Catenin Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: c2bbe
    Species: Human
    Verified Customer | Posted 11/30/2018
  • Human/Mouse/Rat beta -Catenin Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: IPS2 induced pluripotent stem cells
    Species: Mouse
    Verified Customer | Posted 01/25/2018
    beta-Catenin Antibody AF1329
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: kidney
    Species: Rat
    Verified Customer | Posted 04/03/2015
    beta-Catenin Antibody AF1329
  • Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: See PMID 23474492
    Species: Human
    Verified Customer | Posted 01/05/2015

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Showing  1 - 5 of 6 reviews Showing All

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