Detection of Human, Mouse, and Rat CUGBP1/CELF1 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Neuro‑2A mouse neuroblastoma cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat CUGBP1/ CELF1 Monoclonal Antibody (Catalog # MAB9388) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CUGBP1/CELF1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
CUGBP1/CELF1 in Human Skeletal Muscle.
CUGBP1/CELF1 was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Mouse Anti-Human/Mouse/Rat CUGBP1/CELF1 Monoclonal Antibody (Catalog # MAB9388) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CELF-1 is a highly conserved, ubiquitous protein which binds pre-mRNA in the nucleus to mediate alternative splicing to produce alternative transcripts in tissue-specific and developmentally regulated events. In the cytoplasm, CELF-1 binds to GRE-containing transcripts which are rapidly degraded. Additionally, cytoplasmic CELF-1 binds to untranslated regions of transcripts for stability and translation efficiency. Many transcripts targeted by CELF-1 show rapid up-regulation leading to cellular activation or proliferation, while others show down-regulation. CELF-1 has also been shown to localize to cytoplasmic stress granules when cells are stressed. This gene is associated with myotonic dystrophy type 1 (DM1) disease though interactions with the dystrophia myotnica-protein kinase (DMPK) gene.
CUGBP, Elav-Like Family Member 1
Entrez Gene IDs:
10658 (Human); 13046 (Mouse); 362160 (Rat)
BRUNOL2; BRUNOL250 kDa nuclear polyadenylated RNA-binding protein; bruno-like 2; Bruno-like protein 2; CELF1; CUG RNA-binding protein; CUG triplet repeat, RNA binding protein 1; CUG triplet repeat, RNA-binding protein 1; CUG-BP- and ETR-3-like factor 1; CUGBP Elav-like family member 1; CUG-BP; CUGBP, Elav-like family member 1; CUGBP1; CUG-BP1; CUGBP1CUG triplet repeat RNA-binding protein 1; CUGBPCELF-1; D2Wsu101e; Deadenylation factor CUG-BP; EDEN-BP homolog; EDEN-BP; embryo deadenylation element binding protein; Embryo deadenylation element-binding protein homolog; HNAB50; NAB50; NAB50CUG-BP1; NAPOR; nuclear polyadenylated RNA-binding protein, 50-kD; RNA-binding protein BRUNOL-2
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