Human/Mouse/Rat HIF-1 alpha Antibody

Catalog # Availability Size / Price Qty
MAB1536
MAB1536-SP
Detection of Human, Mouse, and Rat HIF‑1 alpha  by Western Blot.
5 Images
Product Details
Citations (25)
FAQs
Supplemental Products
Reviews (1)

Human/Mouse/Rat HIF-1 alpha Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat HIF-1 alpha.
Source
Monoclonal Mouse IgG1 Clone # 241809
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human HIF-1 alpha
Arg575-Asn826
Accession # Q16665.1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
5-25 µg/mL
See below
Immunoprecipitation
1-3 µg/500 µg cell lysate
MCF‑7 human breast cancer cell line treated with CoCl2, see our available Western blot detection antibodies
Knockout Validated
HIF‑1 alpha is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in HIF‑1 alpha knockout HeLa cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human, Mouse, and Rat HIF‑1 alpha  by Western Blot. View Larger

Detection of Human, Mouse, and Rat HIF‑1 alpha by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line, Balb‑3T3 mouse embryonic fibroblast cell line, and PC‑12 rat adrenal pheochromocytoma cell line untreated (-) or treated (+) with 150 μM CoCl2 for 8 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat HIF‑1 alpha Monoclonal Antibody, followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HIF‑1 alpha at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry HIF‑1 alpha   in Human Kidney. View Larger

HIF‑1 alpha in Human Kidney. HIF‑1 alpha was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human/Mouse/Rat HIF‑1 alpha Monoclonal Antibody (Catalog # MAB1536) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western Detection of Human HIF‑1 alpha  by Simple WesternTM. View Larger

Detection of Human HIF‑1 alpha by Simple WesternTM. Simple Western lane view shows lysates of A549 human lung carcinoma cell line untreated (-) or treated (+) with Hypoxia (1% O2), loaded at 0.2 mg/mL. A specific band was detected for HIF‑1 alpha at approximately 116 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat HIF‑1 alpha Monoclonal Antibody (Catalog # MAB1536). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.

Knockout Validated Western  Blot Shows Human HIF‑1 alpha  Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human HIF‑1 alpha Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO overnight. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat HIF‑1 alpha Monoclonal Antibody (Catalog # MAB1536) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIF‑1 alpha at approximately 110 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Knockout Validated HIF‑1 alpha   Specificity is Shown by Immunocytochemistry in Knockout Cell Line. View Larger

HIF‑1 alpha Specificity is Shown by Immunocytochemistry in Knockout Cell Line. HIF‑1 alpha was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with DFO but is not detected in HIF‑1 alpha knockout (KO) HeLa Human Cell Line cell line using Mouse Anti-Human/Mouse/Rat HIF‑1 alpha Monoclonal Antibody (Catalog # MAB1536) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HIF-1 alpha

The hypoxia-inducible transcription factor 1 alpha (HIF-1 alpha ) is the regulated member of the transcription factor heterodimer HIF-1. HIF-1 binds to hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the broad impact of gene regulation by HIF-1 alpha.

Long Name
Hypoxia-inducible Transcription Factor 1 alpha
Entrez Gene IDs
3091 (Human); 15251 (Mouse); 29560 (Rat)
Alternate Names
AINT; anti-HIF-1 alpha; anti-HIF1A; ARNT interacting protein; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; BHLHE78; Class E basic helix-loop-helix protein 78; HIF 1A; HIF1 alpha; HIF-1 alpha; HIF1; HIF1A; HIF-1a; HIF-1alpha; HIF-1-alpha; HIF1-alpha; hypoxia inducible factor 1 alpha subunit, hypoxia inducible factor 1 subunit alpha; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1-alpha; Member of PAS protein 1; member of PAS superfamily 1; MOP1; PAS domain-containing protein 8; PASD8

Product Datasheets

Citations for Human/Mouse/Rat HIF-1 alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

25 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Modeling radiation injury-induced cell death and countermeasure drug responses in a human Gut-on-a-Chip
    Authors: S Jalili-Fir, R Prantil-Ba, A Jiang, R Potla, T Mammoto, JC Weaver, TC Ferrante, HJ Kim, JMS Cabral, O Levy, DE Ingber
    Cell Death Dis, 2018;9(2):223.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Metabolic changes and inflammation in cultured astrocytes from the 5xFAD mouse model of Alzheimer's disease: Alleviation by pantethine
    Authors: M van Gijsel, K Baranger, P Benech, S Rivera, M Khrestchat, M de Reggi, B Gharib
    PLoS ONE, 2017;12(4):e0175369.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. VHL promotes immune response against renal cell carcinoma via NF-?B-dependent regulation of VCAM-1
    Authors: D Labrousse-, E Martínez-A, M Corral-Esc, R Bienes-Mar, J Berridy, L Serrano-Ov, E Conde, ML García-Ber, JM Giménez-Ba, AS Salinas-Sá, R Sánchez-Pr, M Yao, M Lasa, MJ Calzada
    J. Cell Biol, 2017;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Protective effect of zinc preconditioning against renal ischemia reperfusion injury is dose dependent
    Authors: K Rao, K Sethi, J Ischia, L Gibson, L Galea, L Xiao, M Yim, M Chang, N Papa, D Bolton, A Shulkes, GS Baldwin, O Patel
    PLoS ONE, 2017;12(7):e0180028.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Hybrid Cellular Metabolism Coordinated by Zic3 and Esrrb Synergistically Enhances Induction of Naive Pluripotency
    Authors: M Sone, N Morone, T Nakamura, A Tanaka, K Okita, K Woltjen, M Nakagawa, JE Heuser, Y Yamada, S Yamanaka, T Yamamoto
    Cell Metab., 2017;25(5):1103-1117.e6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  6. Mitochondrial complex I deactivation is related to superoxide production in acute hypoxia
    Authors: P Hernansanz, E Ramos, E Navarro, E Parada, N Sánchez-Ló, L Peláez-Agu, JD Cabrera-Ga, D Tello, I Buendia, A Marina, J Egea, MG López, A Bogdanova, A Martínez-R
    Redox Biol, 2017;12(0):1040-1051.
    Species: Bovine
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Silencing HIF-1? induces TET2 expression and augments ascorbic acid induced 5-hydroxymethylation of DNA in human metastatic melanoma cells
    Authors: AP Fischer, SL Miles
    Biochem. Biophys. Res. Commun., 2017;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  8. Ferrochelatase is a therapeutic target for ocular neovascularization
    Authors: HD Basavaraja, RS Sulaiman, X Qi, T Shetty, S Sheik Pran, KL Sishtla, B Lee, J Quigley, S Alkhairy, CM Briggs, K Gupta, B Tang, M Shadmand, MB Grant, ME Boulton, SY Seo, TW Corson
    EMBO Mol Med, 2017;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. XIAP upregulates expression of HIF target genes by targeting HIF1? for Lys63-linked polyubiquitination
    Authors: CV Park, IG Ivanova, NS Kenneth
    Nucleic Acids Res., 2017;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia
    Authors: P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu
    Oncotarget, 2017;8(17):28074-28092.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Phosphoribosylation of Ubiquitin Promotes Serine Ubiquitination and Impairs Conventional Ubiquitination
    Authors: Sagar Bhogaraju
    Cell, 2016;167(6):1636-1649.e13.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Chronic intermittent hypoxia induces cardiac inflammation and dysfunction in a rat obstructive sleep apnea model
    Authors: Qin Wei
    J Biomed Res, 2016;30(6):490-495.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  13. Leptin directly promotes T-cell glycolytic metabolism to drive effector T-cell differentiation in a mouse model of autoimmunity
    Eur J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. NQO1 inhibits proteasome-mediated degradation of HIF-1?
    Nat Commun, 2016;7(0):13593.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. Panobinostat reduces hypoxia-induced cisplatin resistance of non-small cell lung carcinoma cells via HIF-1alpha destabilization.
    Authors: Fischer C, Leithner K, Wohlkoenig C, Quehenberger F, Bertsch A, Olschewski A, Olschewski H, Hrzenjak A
    Mol Cancer, 2015;14(1):4.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-Fr
  16. Dose-dependent effects of allopurinol on human foreskin fibroblast cells and human umbilical vein endothelial cells under hypoxia.
    Authors: Sun Y, George J, Rocha S
    PLoS ONE, 2015;10(4):e0123649.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  17. HIF-1-PHD2 axis controls expression of syndecan 4 in nucleus pulposus cells.
    Authors: Fujita N, Hirose Y, Tran C, Chiba K, Miyamoto T, Toyama Y, Shapiro I, Risbud M
    FASEB J, 2014;28(6):2455-65.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. NF-kappaB enhances hypoxia-driven T-cell immunosuppression via upregulation of adenosine A(2A) receptors.
    Authors: Bruzzese L, Fromonot J, By Y, Durand-Gorde J, Condo J, Kipson N, Guieu R, Fenouillet E, Ruf J
    Cell Signal, 2014;26(5):1060-7.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  19. The P-body component USP52/PAN2 is a novel regulator of HIF1A mRNA stability.
    Authors: Bett J, Ibrahim A, Garg A, Kelly V, Pedrioli P, Rocha S, Hay R
    Biochem J, 2013;451(2):185-94.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  20. Evolutionary conserved regulation of HIF-1beta by NF-kappaB.
    Authors: van Uden P, Kenneth NS, Webster R, Muller HA, Mudie S, Rocha S
    PLoS Genet., 2012;7(1):e1001285.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  21. Hypoxic preconditioning advances CXCR4 and CXCR7 expression by activating HIF-1alpha in MSCs.
    Authors: Liu H, Xue W, Ge G, Luo X, Li Y, Xiang H, Ding X, Tian P, Tian X
    Biochem. Biophys. Res. Commun., 2010;401(4):509-15.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  22. The von Hippel-Lindau Chuvash mutation promotes pulmonary hypertension and fibrosis in mice.
    Authors: Hickey MM, Richardson T, Wang T, Mosqueira M, Arguiri E, Yu H, Yu QC, Solomides CC, Morrisey EE, Khurana TS, Christofidou-Solomidou M, Simon MC
    J. Clin. Invest., 2010;120(3):827-39.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  23. Oxygen tension regulates preosteocyte maturation and mineralization.
    Authors: Zahm AM, Bucaro MA, Srinivas V, Shapiro IM, Adams CS
    Bone, 2008;43(1):25-31.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  24. CYR61 and alphaVbeta5 integrin cooperate to promote invasion and metastasis of tumors growing in preirradiated stroma.
    Authors: Monnier Y, Farmer P, Bieler G, Imaizumi N, Sengstag T, Alghisi GC, Stehle JC, Ciarloni L, Andrejevic-Blant S, Moeckli R, Mirimanoff RO, Goodman SL, Delorenzi M, Ruegg C
    Cancer Res., 2008;68(18):7323-31.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  25. Nucleus pulposus cells express HIF-1alpha under normoxic culture conditions: a metabolic adaptation to the intervertebral disc microenvironment.
    Authors: Risbud MV, Guttapalli A, Stokes DG, Hawkins D, Danielson KG, Schaer TP, Albert TJ, Shapiro IM
    J. Cell. Biochem., 2006;98(1):152-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Cell and Tissue Staining Kits

ExactaChIP Chromatin Immunoprecipitation Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human/Mouse/Rat HIF-1 alpha Antibody

Average Rating: 4 (Based on 1 Review)

5 Star
0%
4 Star
100%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human/Mouse/Rat HIF-1 alpha Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human/Mouse/Rat HIF-1 alpha Antibody
By Anonymous on 08/14/2017
Application: WB Sample Tested: Caco-2 human colorectal adenocarcinoma cell line Species: Human