Human/Mouse/Rat HIF-1 alpha Antibody

  (24 citations)
(1 Review)
    
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Citations (24)
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  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse, and rat HIF-1 alpha.
  • Source
    Monoclonal Mouse IgG1 Clone # 241809
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human HIF-1 alpha
    Arg575-Asn826
    Accession # Q16665.1
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
  • Immunoprecipitation
    1-3 µg/500 µg cell lysate
    MCF‑7 human breast cancer cell line treated with CoCl2, see our available Western blot detection antibodies
  • Knockout Validated
    HIF‑1 alpha is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in HIF‑1 alpha knockout HeLa cell line.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human, Mouse, and Rat HIF‑1 alpha by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line, Balb‑3T3 mouse embryonic fibroblast cell line, and PC‑12 rat adrenal pheochromocytoma cell line untreated (-) or treated (+) with 150 μM CoCl2 for 8 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat HIF‑1 alpha Monoclonal Antibody, followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HIF‑1 alpha at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human HIF‑1 alpha by Simple WesternTM. Simple Western lane view shows lysates of A549 human lung carcinoma cell line untreated (-) or treated (+) with Hypoxia (1% O2), loaded at 0.2 mg/mL. A specific band was detected for HIF‑1 alpha at approximately 116 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat HIF‑1 alpha Monoclonal Antibody (Catalog # MAB1536). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Knockout Validated
Western Blot Shows Human HIF‑1 alpha Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha knockout HeLa cell line (KO) untreated (-) or treated (+) with 1mM DFO overnight. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat HIF‑1 alpha Monoclonal Antibody (Catalog # MAB1536) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIF‑1 alpha at approximately 110 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HIF-1 alpha

The hypoxia-inducible transcription factor 1 alpha (HIF-1 alpha ) is the regulated member of the transcription factor heterodimer HIF-1. HIF-1 binds to hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the broad impact of gene regulation by HIF-1 alpha.

  • Long Name:
    Hypoxia-inducible Transcription Factor 1 alpha
  • Entrez Gene IDs:
    3091 (Human); 15251 (Mouse); 29560 (Rat)
  • Alternate Names:
    ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; BHLHE78; Class E basic helix-loop-helix protein 78; HIF 1A; HIF1 alpha; HIF-1 alpha; HIF1A; HIF-1a; HIF-1-alpha; HIF1-alpha; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcriptionfactor); hypoxia-inducible factor 1-alpha; Member of PAS protein 1; member of PAS superfamily 1; MOP1HIF1-ALPHA; PAS domain-containing protein 8; PASD8alpha subunit (basic helix-loop-helix transcriptionfactor)
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

24 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Modeling radiation injury-induced cell death and countermeasure drug responses in a human Gut-on-a-Chip
    Authors: S Jalili-Fir, R Prantil-Ba, A Jiang, R Potla, T Mammoto, JC Weaver, TC Ferrante, HJ Kim, JMS Cabral, O Levy, DE Ingber
    Cell Death Dis, 2018;9(2):223.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Metabolic changes and inflammation in cultured astrocytes from the 5xFAD mouse model of Alzheimer's disease: Alleviation by pantethine
    Authors: M van Gijsel, K Baranger, P Benech, S Rivera, M Khrestchat, M de Reggi, B Gharib
    PLoS ONE, 2017;12(4):e0175369.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  3. VHL promotes immune response against renal cell carcinoma via NF-?B-dependent regulation of VCAM-1
    Authors: D Labrousse-, E Martínez-A, M Corral-Esc, R Bienes-Mar, J Berridy, L Serrano-Ov, E Conde, ML García-Ber, JM Giménez-Ba, AS Salinas-Sá, R Sánchez-Pr, M Yao, M Lasa, MJ Calzada
    J. Cell Biol, 2017;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. XIAP upregulates expression of HIF target genes by targeting HIF1? for Lys63-linked polyubiquitination
    Authors: CV Park, IG Ivanova, NS Kenneth
    Nucleic Acids Res., 2017;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. Protective effect of zinc preconditioning against renal ischemia reperfusion injury is dose dependent
    Authors: K Rao, K Sethi, J Ischia, L Gibson, L Galea, L Xiao, M Yim, M Chang, N Papa, D Bolton, A Shulkes, GS Baldwin, O Patel
    PLoS ONE, 2017;12(7):e0180028.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  6. HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia
    Authors: P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu
    Oncotarget, 2017;8(17):28074-28092.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  7. Mitochondrial complex I deactivation is related to superoxide production in acute hypoxia
    Authors: P Hernansanz, E Ramos, E Navarro, E Parada, N Sánchez-Ló, L Peláez-Agu, JD Cabrera-Ga, D Tello, I Buendia, A Marina, J Egea, MG López, A Bogdanova, A Martínez-R
    Redox Biol, 2017;12(0):1040-1051.
    Species: Bovine
    Sample Type: Cell Lysates
    Application: WB
  8. Ferrochelatase is a therapeutic target for ocular neovascularization
    Authors: HD Basavaraja, RS Sulaiman, X Qi, T Shetty, S Sheik Pran, KL Sishtla, B Lee, J Quigley, S Alkhairy, CM Briggs, K Gupta, B Tang, M Shadmand, MB Grant, ME Boulton, SY Seo, TW Corson
    EMBO Mol Med, 2017;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  9. Chronic intermittent hypoxia induces cardiac inflammation and dysfunction in a rat obstructive sleep apnea model
    Authors: Qin Wei
    J Biomed Res, 2016;30(6):490-495.
    Species: Rat
    Sample Type: Tissue Homgenates
    Application: WB
  10. Phosphoribosylation of Ubiquitin Promotes Serine Ubiquitination and Impairs Conventional Ubiquitination
    Authors: Sagar Bhogaraju
    Cell, 2016;167(6):1636-1649.e13.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  11. Leptin directly promotes T-cell glycolytic metabolism to drive effector T-cell differentiation in a mouse model of autoimmunity
    Eur J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  12. NQO1 inhibits proteasome-mediated degradation of HIF-1?
    Nat Commun, 2016;7(0):13593.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  13. Panobinostat reduces hypoxia-induced cisplatin resistance of non-small cell lung carcinoma cells via HIF-1alpha destabilization.
    Authors: Fischer C, Leithner K, Wohlkoenig C, Quehenberger F, Bertsch A, Olschewski A, Olschewski H, Hrzenjak A
    Mol Cancer, 2015;14(1):4.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC Fresh
  14. Dose-dependent effects of allopurinol on human foreskin fibroblast cells and human umbilical vein endothelial cells under hypoxia.
    Authors: Sun Y, George J, Rocha S
    PLoS ONE, 2015;10(4):e0123649.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  15. NF-kappaB enhances hypoxia-driven T-cell immunosuppression via upregulation of adenosine A(2A) receptors.
    Authors: Bruzzese L, Fromonot J, By Y, Durand-Gorde J, Condo J, Kipson N, Guieu R, Fenouillet E, Ruf J
    Cell Signal, 2014;26(5):1060-7.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  16. HIF-1-PHD2 axis controls expression of syndecan 4 in nucleus pulposus cells.
    Authors: Fujita N, Hirose Y, Tran C, Chiba K, Miyamoto T, Toyama Y, Shapiro I, Risbud M
    FASEB J, 2014;28(6):2455-65.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  17. The P-body component USP52/PAN2 is a novel regulator of HIF1A mRNA stability.
    Authors: Bett J, Ibrahim A, Garg A, Kelly V, Pedrioli P, Rocha S, Hay R
    Biochem J, 2013;451(2):185-94.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  18. Evolutionary conserved regulation of HIF-1beta by NF-kappaB.
    Authors: van Uden P, Kenneth NS, Webster R, Muller HA, Mudie S, Rocha S
    PLoS Genet., 2012;7(1):e1001285.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  19. Hypoxic preconditioning advances CXCR4 and CXCR7 expression by activating HIF-1alpha in MSCs.
    Authors: Liu H, Xue W, Ge G, Luo X, Li Y, Xiang H, Ding X, Tian P, Tian X
    Biochem. Biophys. Res. Commun., 2010;401(4):509-15.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  20. The von Hippel-Lindau Chuvash mutation promotes pulmonary hypertension and fibrosis in mice.
    Authors: Hickey MM, Richardson T, Wang T, Mosqueira M, Arguiri E, Yu H, Yu QC, Solomides CC, Morrisey EE, Khurana TS, Christofidou-Solomidou M, Simon MC
    J. Clin. Invest., 2010;120(3):827-39.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  21. CYR61 and alphaVbeta5 integrin cooperate to promote invasion and metastasis of tumors growing in preirradiated stroma.
    Authors: Monnier Y, Farmer P, Bieler G, Imaizumi N, Sengstag T, Alghisi GC, Stehle JC, Ciarloni L, Andrejevic-Blant S, Moeckli R, Mirimanoff RO, Goodman SL, Delorenzi M, Ruegg C
    Cancer Res., 2008;68(18):7323-31.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  22. Oxygen tension regulates preosteocyte maturation and mineralization.
    Authors: Zahm AM, Bucaro MA, Srinivas V, Shapiro IM, Adams CS
    Bone, 2008;43(1):25-31.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  23. Early induction of neuronal lipocalin-type prostaglandin D synthase after hypoxic-ischemic injury in developing brains.
    Authors: Taniguchi H, Mohri I, Okabe-Arahori H, Kanekiyo T, Kagitani-Shimono K, Wada K, Urade Y, Nakayama M, Ozono K, Taniike M
    Neurosci. Lett., 2007;420(1):39-44.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  24. Nucleus pulposus cells express HIF-1alpha under normoxic culture conditions: a metabolic adaptation to the intervertebral disc microenvironment.
    Authors: Risbud MV, Guttapalli A, Stokes DG, Hawkins D, Danielson KG, Schaer TP, Albert TJ, Shapiro IM
    J. Cell. Biochem., 2006;98(1):152-9.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
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Average Rating: 4 (Based on 1 review)

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We have 1 review tested in 1 application: Western Blot.

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Western Blot Human/Mouse/Rat HIF-1 alpha  Antibody MAB1536 Array MAB1536
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Very Good
 WB Human Anonymous 08/14/2017
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Western Blot Human/Mouse/Rat HIF-1 alpha  Antibody MAB1536
Western Blot: Human/Mouse/Rat HIF-1 alpha Antibody [MAB1536].

Summary

Application Western Blot
Sample Tested Caco-2 human colorectal adenocarcinoma cell line
Species Human

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