Human/Mouse/Rat PI 3‑Kinase p55 gamma Antibody

Name: Human/Mouse/Rat PI 3‑Kinase p55 gamma Antibody
Brand: R&D Systems
SKU: MAB6638
Price: 459 USD
Availability: InStock

R&D Systems | Catalog # MAB6638

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
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Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # 668619

View all PI 3-Kinase p55 gamma Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human p55 gamma
Lys251-Gly378
Accession # Q92569

Specificity

Detects human, mouse, and rat PI 3‑Kinase p55 gamma in Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) PIK3R1 (aa 519‑644) and no cross-reactivity with rhPIK3R1 (aa 328-431), rhPIK3R2 (aa 325-428), rhPIK3R2 (aa 516-642), rhPIK3C3 (aa 419‑887), rhPIK3R4 (aa 58-230), or rhPIK3R5 (aa 530-726) is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human/Mouse/Rat PI 3‑Kinase p55 gamma Antibody

Detection of Human, Mouse, and Rat PI 3‑Kinase p55 gamma by Western Blot.

Western blot shows lysates of HEK293 human embryonic kidney cell line, NIH-3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF Membrane was probed with 1 µg/mL of Human/Mouse/Rat PI 3-Kinase p55 gamma Monoclonal Antibody (Catalog # MAB6638) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for p55 gamma at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat PI 3‑Kinase p55 gamma Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: HEK293 human embryonic kidney cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: PI 3-Kinase p55 gamma

p55 gamma (Protein of 55 kDa, gamma subunit; also PI3-kinase regulatory subunit gamma and p55PIK) is a 55-57 kDa member of the PI3K p85 family of regulatory subunits. It is widely expressed, and serves as a recruitment subunit for the p110 catalytic subunit of PI3 kinase. p55 gamma /p55PIK should not be confused with the 55 kDa alpha splice variant of the p85a gene (75% amino acid [aa] identity). Human p55 gamma is 461 aa in length. It contains a unique 34 aa N-terminus that binds Rb and prevents cycle progression, followed by a Pro-rich region (aa 35-44) and two SH2 domains that bind the p110 catalytic subunit (aa 65-160 and 358-452). Based on mouse, it is likely that alternate start sites at Met8 and Met32 generate 54 and 50 kDa protein products, respectively. There is also a potential for a start site 18 aa upstream of the standard start site, as well as a splice form that shows a deletion of aa 256-314 that may be accompanied by an additional deletion of aa 36-71. Over aa 251-378, human p55 gamma shares 94% aa identity with mouse p55 gamma.