Human/Mouse/Rat PI 3-Kinase p55 gamma Antibody

(1 citations)   
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse, and rat PI 3‑Kinase p55 gamma in Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) PIK3R1 (aa 519‑644) and no cross-reactivity with rhPIK3R1 (aa 328-431), rhPIK3R2 (aa 325-428), rhPIK3R2 (aa 516-642), rhPIK3C3 (aa 419‑887), rhPIK3R4 (aa 58-230), or rhPIK3R5 (aa 530-726) is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 668619
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human p55 gamma
    Lys251-Gly378
    Accession # Q92569
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human, Mouse, and Rat PI 3‑Kinase p55 gamma  by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF Membrane was probed with 1 µg/mL of Human/Mouse/Rat PI 3‑Kinase p55 gamma Monoclonal Antibody (Catalog # MAB6638) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for p55 gamma at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PI 3-Kinase p55 gamma
p55 gamma (Protein of 55 kDa, gamma subunit; also PI3-kinase regulatory subunit gamma and p55PIK) is a 55-57 kDa member of the PI3K p85 family of regulatory subunits. It is widely expressed, and serves as a recruitment subunit for the p110 catalytic subunit of PI3 kinase. p55 gamma /p55PIK should not be confused with the 55 kDa alpha splice variant of the p85a gene (75% amino acid [aa] identity). Human p55 gamma is 461 aa in length. It contains a unique 34 aa N-terminus that binds Rb and prevents cycle progression, followed by a Pro-rich region (aa 35-44) and two SH2 domains that bind the p110 catalytic subunit (aa 65-160 and 358-452). Based on mouse, it is likely that alternate start sites at Met8 and Met32 generate 54 and 50 kDa protein products, respectively. There is also a potential for a start site 18 aa upstream of the standard start site, as well as a splice form that shows a deletion of aa 256-314 that may be accompanied by an additional deletion of aa 36-71. Over aa 251-378, human p55 gamma shares 94% aa identity with mouse p55 gamma.
  • Long Name:
    PI 3-Kinase p55 gamma
  • Entrez Gene IDs:
    8503 (Human); 18710 (Mouse); 60664 (Rat)
  • Alternate Names:
    p55PIK; PI 3Kinase p55 gamma; PI 3-Kinase p55 gamma; PIK3R3
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. A large scale Huntingtin protein interaction network implicates Rho GTPase signaling pathways in Huntington disease.
    Authors: Tourette, Cendrine, Li, Biao, Bell, Russell, O'Hare, Shannon, Kaltenbach, Linda S, Mooney, Sean D, Hughes, Robert E
    J Biol Chem, 0;289(10):6709-26.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Isotype Control

Ctrl MAB004 32  
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Mouse IgG2B Isotype Control

Ctrl MAB0041 7  
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Mouse IgG2B Isotype Control

Ctrl MAB0042 6
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133 1  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Rat Anti-Mouse IgG2B PE-conjugated Antibody

Flow F0132  
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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

Flow F0134 1
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Mouse IgG2B Antibody

Flow MAB0043  
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