Human/Mouse/Rat PI 3-Kinase p55 gamma Antibody

Catalog # Availability Size / Price Qty
MAB6638
MAB6638-SP
Human/Mouse/Rat PI 3-Kinase p55 gamma  Antibody in Data
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Human/Mouse/Rat PI 3-Kinase p55 gamma Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat PI 3‑Kinase p55 gamma in Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) PIK3R1 (aa 519‑644) and no cross-reactivity with rhPIK3R1 (aa 328-431), rhPIK3R2 (aa 325-428), rhPIK3R2 (aa 516-642), rhPIK3C3 (aa 419‑887), rhPIK3R4 (aa 58-230), or rhPIK3R5 (aa 530-726) is observed.
Source
Monoclonal Mouse IgG2B Clone # 668619
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human p55 gamma
Lys251-Gly378
Accession # Q92569
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human, Mouse, and Rat PI 3-Kinase p55? antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat PI 3‑Kinase p55 gamma by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line, NIH-3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF Membrane was probed with 1 µg/mL of Human/Mouse/Rat PI 3-Kinase p55 gamma Monoclonal Antibody (Catalog # MAB6638) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for p55 gamma at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PI 3-Kinase p55 gamma

p55 gamma (Protein of 55 kDa, gamma subunit; also PI3-kinase regulatory subunit gamma and p55PIK) is a 55-57 kDa member of the PI3K p85 family of regulatory subunits. It is widely expressed, and serves as a recruitment subunit for the p110 catalytic subunit of PI3 kinase. p55 gamma /p55PIK should not be confused with the 55 kDa alpha splice variant of the p85a gene (75% amino acid [aa] identity). Human p55 gamma is 461 aa in length. It contains a unique 34 aa N-terminus that binds Rb and prevents cycle progression, followed by a Pro-rich region (aa 35-44) and two SH2 domains that bind the p110 catalytic subunit (aa 65-160 and 358-452). Based on mouse, it is likely that alternate start sites at Met8 and Met32 generate 54 and 50 kDa protein products, respectively. There is also a potential for a start site 18 aa upstream of the standard start site, as well as a splice form that shows a deletion of aa 256-314 that may be accompanied by an additional deletion of aa 36-71. Over aa 251-378, human p55 gamma shares 94% aa identity with mouse p55 gamma.

Long Name
PI 3-Kinase p55 gamma
Entrez Gene IDs
8503 (Human); 18710 (Mouse); 60664 (Rat)
Alternate Names
DKFZp686P05226; FLJ41892; p55; p55-GAMMA; p55PIK; Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma; phosphatidylinositol 3-kinase regulatory subunit gamma; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 3 (p55, gamma); phosphoinositide-3-kinase, regulatory subunit 3 (gamma); PI 3Kinase p55 gamma; PI 3-Kinase p55 gamma; PI3K regulatory subunit gamma; PI3-kinase regulatory subunit gamma; PI3-kinase subunit p55-gamma; PIK3R3; PtdIns-3-kinase regulatory subunit gamma; ptdIns-3-kinase regulatory subunit p55-gamma; regulatory subunit, polypeptide 3 (p55, gamma)

Product Datasheets

Citation for Human/Mouse/Rat PI 3-Kinase p55 gamma Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. A large scale Huntingtin protein interaction network implicates Rho GTPase signaling pathways in Huntington disease.
    Authors: Tourette, Cendrine, Li, Biao, Bell, Russell, O'Hare, Shannon, Kaltenbach, Linda S, Mooney, Sean D, Hughes, Robert E
    J Biol Chem, 0;289(10):6709-26.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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