Human/Mouse/Rat TRAF-2 Antibody

Catalog # Availability Size / Price Qty
MAB3277
MAB3277-SP
Human/Mouse/Rat TRAF-2 Antibody in Data
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Product Details
Citations (4)
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Human/Mouse/Rat TRAF-2 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat TRAF-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human TRAF-1, -3, -4, -5, or -6 is observed.
Source
Monoclonal Mouse IgG2A Clone # 324522
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human TRAF-2
Met1-Leu501
Accession # Q12933
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Immunocytochemistry
3-25 µg/mL
See below
Knockout Validated
TRAF‑2 is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in TRAF‑2 knockout HEK293T cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human/Mouse/Rat TRAF‑2 antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat TRAF‑2 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, SW3T3 mouse contact inhibited fibroblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.25 µg/mL Mouse Anti-Human/Mouse/Rat TRAF-2 Monoclonal Antibody (Catalog # MAB3277) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human TRAF-1, -2, -3, -4, -5, and -6 (2 ng/lane) were included. A specific band for TRAF-2 was detected at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunocytochemistry TRAF-2 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

TRAF‑2 in HeLa Human Cell Line. TRAF-2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat TRAF-2 Monoclonal Antibody (Catalog # MAB3277) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Knockout Validated Western Blot Shows Human TRAF-2 Antibody Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human TRAF‑2 Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and TRAF-2 knockout HEK293T cell line (KO). PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/Mouse/Rat TRAF-2 Monoclonal Antibody (Catalog # MAB3277) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TRAF-2 at approximately 62 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRAF-2

Tumor Necrosis Factor (TNF) Receptor-Associated Factors (TRAFs) are a family of adaptor proteins that interact with a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress response. TRAFs were identified by their ability to form complexes with TNF receptor superfamily members but more recently are reported to also bind to Toll/IL-1 receptor family members and mediate cellular signaling. Six members of the TRAF family have been identified. All TRAF proteins have a homologous C-terminal TRAF domain that can bind the cytoplasmic domain of receptors as well as other TRAFs. TRAFs 2‑6 have N-terminal RING and zinc finger domains that are involved in signaling downstream events. TRAF-2, also known as TNF Receptor-Associated Protein (TRAP), is a 501 amino acid, 56 kDa protein which interacts with the cytoplasmic domain of TNFR1, TNFR2 and CD40 to mediate the activation of NF-kappa-B and AP-1 family of transcription factors. TRAF-2 can self-associate as well as form heterodimers with TRAF-1.

Long Name
TNF Receptor-Associated Factor 2
Entrez Gene IDs
7186 (Human); 22030 (Mouse)
Alternate Names
E3 ubiquitin-protein ligase TRAF2; EC 6.3.2.-; MGC:45012; TNF receptor-associated factor 2; TRAF2; TRAF-2; TRAP3; TRAP3TRAP; tumor necrosis factor type 2 receptor associated protein 3; Tumor necrosis factor type 2 receptor-associated protein 3

Product Datasheets

Citations for Human/Mouse/Rat TRAF-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. NEDD4 ubiquitinates TRAF3 to promote CD40-mediated AKT activation.
    Authors: Fang, Di-Feng, He, Kun, Wang, Na, Sang, Zhi-Hong, Qiu, Xin, Xu, Guang, Jian, Zhao, Liang, Bing, Li, Tao, Li, Hui-Yan, Li, Ai-Ling, Zhou, Tao, Gong, Wei-Li, Yang, Baoli, Karin, Michael, Zhang, Xue-Min, Li, Wei-Hua
    Nat Commun, 2014;5(0):4513.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Zinc-finger protein 91 plays a key role in LIGHT-induced activation of non-canonical NF-kappaB pathway.
    Authors: Jin HR, Jin X, Lee JJ
    Biochem. Biophys. Res. Commun., 2010;400(4):581-6.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. IL-6 cytoprotection in hyperoxic acute lung injury occurs via suppressor of cytokine signaling-1-induced apoptosis signal-regulating kinase-1 degradation.
    Authors: Kolliputi N, Waxman AB
    Am. J. Respir. Cell Mol. Biol., 2009;40(3):314-24.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. The function of TRADD in signaling through tumor necrosis factor receptor 1 and TRIF-dependent Toll-like receptors.
    Authors: Pobezinskaya YL, Kim YS, Choksi S, Morgan MJ, Li T, Liu C, Liu Z
    Nat. Immunol., 2008;9(9):1047-54.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

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Cell and Tissue Staining Kits

Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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