Human/Mouse/Rat Vinculin Antibody

Catalog # Availability Size / Price Qty
MAB6896
MAB6896-SP
Detection of Human Vinculin by Western Blot.
3 Images
Product Details
Citations (12)
FAQs
Supplemental Products
Reviews (5)

Human/Mouse/Rat Vinculin Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human Vinculin in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2A Clone # 728526
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Vinculin
Lys1020-Gln1134
Accession # P18206
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Complete Your Experiment

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Simple Western
20 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Vinculin antibody by Western Blot. View Larger

Detection of Human Vinculin by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Vinculin Monoclonal Antibody (Catalog # MAB6896) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Vinculin at approximately 124 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Vinculin antibody in Human Uterus by Immunohistochemistry (IHC-P). View Larger

Vinculin in Human Uterus. Vinculin was detected in immersion fixed paraffin-embedded sections of human uterus using Mouse Anti-Human Vinculin Monoclonal Antibody (Catalog # MAB6896) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to smooth muscle cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Human Vinculin antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human Vinculin by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and PC‑3 human prostate cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Vinculin at approximately 122 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse/Rat Vinculin Monoclonal Antibody (Catalog # MAB6896). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Vinculin

VCL (vinculin) is a 117 kDa actin binding cytoskeletal phosphoprotein found in adherens-type cell-cell and cell-matrix junctions. The 1066 amino acid (aa) human vinculin contains an N-terminal globular head (aa 2-835) with a talin interaction domain (aa 168-208) and three tandem repeats (aa 259-589), a pro-rich linker region (aa 826-878), and a C-terminal tail that facilitates phospholipid membrane insertion (aa 1003-1134). An 1134 aa form termed metavinculin contains 68 aa inserted after aa 915 and mainly occurs in cardiac, skeletal and smooth muscle. Human, mouse and rat vinculin share 100% aa sequence identity within the region used as an immunogen.

Long Name
Metavinculin
Entrez Gene IDs
7414 (Human); 22330 (Mouse); 305679 (Rat)
Alternate Names
CMD1W; CMH15; metavinculin; MVCL; VCL; Vinculin

Product Datasheets

You must select a language.

x

Citations for Human/Mouse/Rat Vinculin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Non-selective TRPC channel inhibition and suppression of aminoglycoside-induced premature termination codon readthrough by the small molecule AC1903
    Authors: A Baradaran-, CC Bauer, IB Pickles, S Hosseini-F, AD Balgi, K Choi, DM Linley, DJ Beech, M Roberge, RS Bon
    The Journal of Biological Chemistry, 2022;298(2):101546.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Arachidin-1, a Prenylated Stilbenoid from Peanut, Induces Apoptosis in Triple-Negative Breast Cancer Cells
    Authors: S Mohammadho, LC Ho, L Fang, J Xu, F Medina-Bol
    International Journal of Molecular Sciences, 2022;23(3):.
    Species: Human
    Sample Types: Cell Lysates
  3. Effect of small molecule eRF3 degraders on premature termination codon readthrough
    Authors: A Baradaran-, AD Balgi, S Hosseini-F, K Choi, C Has, M Roberge
    Nucleic Acids Research, 2021;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Filamentous tangles with nemaline rods in MYH2 myopathy: a novel phenotype
    Authors: NN Madigan, MJ Polzin, G Cui, T Liewluck, MH Alsharabat, CJ Klein, AJ Windebank, G Mer, M Milone
    Acta neuropathologica communications, 2021;9(1):79.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. Trauma-induced heme release increases susceptibility to bacterial infection
    Authors: GR Lee, D Gallo, RW Alves de S, S Tiwari-Hec, E Csizmadia, JD Harbison, S Shankar, V Banner-Goo, MB Yaffe, MS Longhi, CJ Hauser, LE Otterbein
    JCI Insight, 2021;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  6. Small molecule Y-320 stimulates ribosome biogenesis, protein synthesis, and aminoglycoside-induced premature termination codon readthrough
    Authors: S Hosseini-F, A Baradaran-, C Zimmerman, K Choi, S Flibotte, M Roberge
    PloS Biology, 2021;19(5):e3001221.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. A Dynamic, Split-Luciferase-Based Mini-G Protein Sensor to Functionally Characterize Ligands at All Four Histamine Receptor Subtypes
    Authors: C Höring, U Seibel, K Tropmann, L Grätz, D Mönnich, S Pitzl, G Bernhardt, S Pockes, A Strasser
    Int J Mol Sci, 2020;21(22):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. SILAC Analysis Reveals Increased Secretion of Hemostasis-Related Factors by Senescent Cells
    Authors: CD Wiley, S Liu, C Limbad, AM Zawadzka, J Beck, M Demaria, R Artwood, F Alimirah, JA Lopez-Domi, C Kuehnemann, SR Danielson, N Basisty, HG Kasler, TR Oron, PY Desprez, SD Mooney, BW Gibson, B Schilling, J Campisi, P Kapahi
    Cell Rep, 2019;28(13):3329-3337.e5.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  9. Establishing a Split Luciferase Assay for Proteinkinase G (PKG) Interaction Studies
    Authors: A Schramm, P Mueller-Th, T Littmann, M Harloff, T Ozawa, J Schlossman
    Int J Mol Sci, 2018;19(4):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  10. Gentamicin B1 is a minor gentamicin component with major nonsense mutation suppression activity
    Authors: A Baradaran-, J Niesser, AD Balgi, K Choi, C Zimmerman, AP South, HJ Anderson, NC Strynadka, MB Bally, M Roberge
    Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot Control
  11. Increased reliance on muscle based thermogenesis upon acute minimization of brown adipose tissue function
    Authors: Naresh C Bal
    J Biol Chem, 2016;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  12. Novel small molecules potentiate premature termination codon readthrough by aminoglycosides.
    Authors: Alireza Baradaran-, Aruna D Balgi, Carla Zimmerman, Kunho Choi, Fahimeh S Shidmooss, Jason S Tan, Célia Bergeaud, Alexandra Krause, Stéphane Flibotte, Yoko Shimizu, Hilary J Anderson, Vincent Mouly, Eric Jan, Tom Pfeifer, James B Jaquith, Michel Roberge
    Nucleic Acids Research, 2016;0(0):1362-4962.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Human/Mouse/Rat Vinculin Antibody

Average Rating: 4.6 (Based on 5 Reviews)

5 Star
60%
4 Star
40%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human/Mouse/Rat Vinculin Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human/Mouse/Rat Vinculin Antibody
By Anonymous on 08/27/2021
Application: ICC/IF Sample Tested: Human osteoblast-like cells (MG63) Species: Human

Human/Mouse/Rat Vinculin Antibody
By Anonymous on 01/25/2018
Application: WB Sample Tested: IPS2 induced pluripotent stem cells Species: Mouse

Human/Mouse/Rat Vinculin Antibody
By Anonymous on 12/28/2017
Application: ICC/IF Sample Tested: HMVEC human microvascular endothelial cells Species: Human

Human/Mouse/Rat Vinculin Antibody
By Michele Torino on 12/07/2017
Application: WB Sample Tested: MCF 10A human breast epithelial cell line,U2OS human osteosarcoma cell line Species: Human

Human/Mouse/Rat Vinculin Antibody
By Julie Van on 08/23/2017
Application: WB Sample Tested: Kidney tissue Species: Mouse

- 1:3000 dilution of sample size stock
- used milk for block and Ab dilution
- 10ug of total protein loaded in each well