Human/Mouse/Rat Vinculin Antibody
Human/Mouse/Rat Vinculin Antibody Summary
Lys1020-Gln1134
Accession # P18206
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Vinculin by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Vinculin Monoclonal Antibody (Catalog # MAB6896) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Vinculin at approximately 124 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Vinculin in Human Uterus. Vinculin was detected in immersion fixed paraffin-embedded sections of human uterus using Mouse Anti-Human Vinculin Monoclonal Antibody (Catalog # MAB6896) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to smooth muscle cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of Human Vinculin by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and PC‑3 human prostate cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Vinculin at approximately 122 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse/Rat Vinculin Monoclonal Antibody (Catalog # MAB6896). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Vinculin
VCL (vinculin) is a 117 kDa actin binding cytoskeletal phosphoprotein found in adherens-type cell-cell and cell-matrix junctions. The 1066 amino acid (aa) human vinculin contains an N-terminal globular head (aa 2-835) with a talin interaction domain (aa 168-208) and three tandem repeats (aa 259-589), a pro-rich linker region (aa 826-878), and a C-terminal tail that facilitates phospholipid membrane insertion (aa 1003-1134). An 1134 aa form termed metavinculin contains 68 aa inserted after aa 915 and mainly occurs in cardiac, skeletal and smooth muscle. Human, mouse and rat vinculin share 100% aa sequence identity within the region used as an immunogen.
Product Datasheets
Citations for Human/Mouse/Rat Vinculin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
12
Citations: Showing 1 - 10
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Non-selective TRPC channel inhibition and suppression of aminoglycoside-induced premature termination codon readthrough by the small molecule AC1903
Authors: A Baradaran-, CC Bauer, IB Pickles, S Hosseini-F, AD Balgi, K Choi, DM Linley, DJ Beech, M Roberge, RS Bon
The Journal of Biological Chemistry, 2022;298(2):101546.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Arachidin-1, a Prenylated Stilbenoid from Peanut, Induces Apoptosis in Triple-Negative Breast Cancer Cells
Authors: S Mohammadho, LC Ho, L Fang, J Xu, F Medina-Bol
International Journal of Molecular Sciences, 2022;23(3):.
Species: Human
Sample Types: Cell Lysates
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Effect of small molecule eRF3 degraders on premature termination codon readthrough
Authors: A Baradaran-, AD Balgi, S Hosseini-F, K Choi, C Has, M Roberge
Nucleic Acids Research, 2021;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Filamentous tangles with nemaline rods in MYH2 myopathy: a novel phenotype
Authors: NN Madigan, MJ Polzin, G Cui, T Liewluck, MH Alsharabat, CJ Klein, AJ Windebank, G Mer, M Milone
Acta neuropathologica communications, 2021;9(1):79.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
Trauma-induced heme release increases susceptibility to bacterial infection
Authors: GR Lee, D Gallo, RW Alves de S, S Tiwari-Hec, E Csizmadia, JD Harbison, S Shankar, V Banner-Goo, MB Yaffe, MS Longhi, CJ Hauser, LE Otterbein
JCI Insight, 2021;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Small molecule Y-320 stimulates ribosome biogenesis, protein synthesis, and aminoglycoside-induced premature termination codon readthrough
Authors: S Hosseini-F, A Baradaran-, C Zimmerman, K Choi, S Flibotte, M Roberge
PloS Biology, 2021;19(5):e3001221.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
A Dynamic, Split-Luciferase-Based Mini-G Protein Sensor to Functionally Characterize Ligands at All Four Histamine Receptor Subtypes
Authors: C Höring, U Seibel, K Tropmann, L Grätz, D Mönnich, S Pitzl, G Bernhardt, S Pockes, A Strasser
Int J Mol Sci, 2020;21(22):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
SILAC Analysis Reveals Increased Secretion of Hemostasis-Related Factors by Senescent Cells
Authors: CD Wiley, S Liu, C Limbad, AM Zawadzka, J Beck, M Demaria, R Artwood, F Alimirah, JA Lopez-Domi, C Kuehnemann, SR Danielson, N Basisty, HG Kasler, TR Oron, PY Desprez, SD Mooney, BW Gibson, B Schilling, J Campisi, P Kapahi
Cell Rep, 2019;28(13):3329-3337.e5.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Establishing a Split Luciferase Assay for Proteinkinase G (PKG) Interaction Studies
Authors: A Schramm, P Mueller-Th, T Littmann, M Harloff, T Ozawa, J Schlossman
Int J Mol Sci, 2018;19(4):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Gentamicin B1 is a minor gentamicin component with major nonsense mutation suppression activity
Authors: A Baradaran-, J Niesser, AD Balgi, K Choi, C Zimmerman, AP South, HJ Anderson, NC Strynadka, MB Bally, M Roberge
Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot Control -
Increased reliance on muscle based thermogenesis upon acute minimization of brown adipose tissue function
Authors: Naresh C Bal
J Biol Chem, 2016;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Novel small molecules potentiate premature termination codon readthrough by aminoglycosides.
Authors: Alireza Baradaran-, Aruna D Balgi, Carla Zimmerman, Kunho Choi, Fahimeh S Shidmooss, Jason S Tan, Célia Bergeaud, Alexandra Krause, Stéphane Flibotte, Yoko Shimizu, Hilary J Anderson, Vincent Mouly, Eric Jan, Tom Pfeifer, James B Jaquith, Michel Roberge
Nucleic Acids Research, 2016;0(0):1362-4962.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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Reviews for Human/Mouse/Rat Vinculin Antibody
Average Rating: 4.6 (Based on 5 Reviews)
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- 1:3000 dilution of sample size stock
- used milk for block and Ab dilution
- 10ug of total protein loaded in each well
