Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western

Cited:

Western Blot, Simple Western, Western Blot Control

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 728526
View all Vinculin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Vinculin
Lys1020-Gln1134
Accession # P18206

Specificity

Detects human Vinculin in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human/Mouse/Rat Vinculin Antibody

Detection of Human Vinculin antibody by Western Blot.

Detection of Human Vinculin by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Vinculin Monoclonal Antibody (Catalog # MAB6896) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Vinculin at approximately 124 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Vinculin antibody in Human Uterus by Immunohistochemistry (IHC-P).

Vinculin in Human Uterus.

Vinculin was detected in immersion fixed paraffin-embedded sections of human uterus using Mouse Anti-Human Vinculin Monoclonal Antibody (Catalog # MAB6896) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to smooth muscle cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human Vinculin antibody by Simple WesternTM.

Detection of Human Vinculin by Simple WesternTM.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and PC‑3 human prostate cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Vinculin at approximately 122 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse/Rat Vinculin Monoclonal Antibody (Catalog # MAB6896). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Human Vinculin by Western Blot

Detection of Human Vinculin by Western Blot

Cr and Ni exposure increases tau levels and phosphorylation in iNeurons and RA-differentiated SH-SY5Y cells. (a,b) iPSCs carrying the R406W tau mutation and isogenic controls were differentiated into iNeurons and treated with Cr (5 µM) and Ni (800 µM) for 72 hours. The levels of total (a) and phospho-tauSer396/404 (PHF-1) (b) tau were measured by western blot. GAPDH and vinculin were used as loading controls. Images show representative immunoblots comparing tau and phospho-tau levels in control and mutant iNeurons before and after Cr and Ni treatment. Plots represents the average ± SEM of 3 independent experiments. (c,d) RA-differentiated SH-SY5Y cells were treated with Cr (2.5 µM) and Nickel (200 µM) for 24 hours before protein extraction. Images show representative immunoblots comparing the levels total tau (c) and phospho-tauSer396/404 (PHF-1) tau (d) before and after heavy metals treatment. GAPDH and vinculin were used as loading control. Plots represent the average ± SEM of 4 independent experiments. Statistical significance was determined by two‐ANOVA followed by Bonferroni’s test for multiple comparisons or one-way ANOVA using GraphPad Prism 6. p-values comparing untreated and heavy metal treated cells are included in the graphs. Statistical significance was considered when p-value ≤ 0.05. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31953414), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Vinculin by Western Blot

Detection of Human Vinculin by Western Blot

Cr and Ni exposure increases tau levels and phosphorylation in iNeurons and RA-differentiated SH-SY5Y cells. (a,b) iPSCs carrying the R406W tau mutation and isogenic controls were differentiated into iNeurons and treated with Cr (5 µM) and Ni (800 µM) for 72 hours. The levels of total (a) and phospho-tauSer396/404 (PHF-1) (b) tau were measured by western blot. GAPDH and vinculin were used as loading controls. Images show representative immunoblots comparing tau and phospho-tau levels in control and mutant iNeurons before and after Cr and Ni treatment. Plots represents the average ± SEM of 3 independent experiments. (c,d) RA-differentiated SH-SY5Y cells were treated with Cr (2.5 µM) and Nickel (200 µM) for 24 hours before protein extraction. Images show representative immunoblots comparing the levels total tau (c) and phospho-tauSer396/404 (PHF-1) tau (d) before and after heavy metals treatment. GAPDH and vinculin were used as loading control. Plots represent the average ± SEM of 4 independent experiments. Statistical significance was determined by two‐ANOVA followed by Bonferroni’s test for multiple comparisons or one-way ANOVA using GraphPad Prism 6. p-values comparing untreated and heavy metal treated cells are included in the graphs. Statistical significance was considered when p-value ≤ 0.05. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31953414), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Vinculin by Western Blot

Detection of Human Vinculin by Western Blot

Cr and Ni exposure induces apoptotic cell death in SH-SY5Y cells. Non-differentiated and RA-differentiated SH-SY5Y cells were treated with Cr (2.5 µM) and Ni (200 µM) for 24 hours. Whole cell lysates were collected in order to analyze by western blot the levels of cleaved caspase-3 protein (a), the ratio of anti-apoptotic protein Bcl2 to pro-apoptotic protein Bax (b) and the cleavage and activation of caspase-9 (c). Vinculin and GAPDH were used as loading controls. (a) Representative immunoblot showing the presence of the 17/19KD fragment of caspase-3 after Cr and Ni exposure. (b) Representative immunoblots showing the levels of Bcl2 and Bax proteins after heavy metal exposure. The activation of the intrinsic/mitochondrial apoptosis pathway was assessed by the decrease of the ratio of Bcl2 to full length Bax and the presence of a cleavage form of Bax protein. The plot represent the average ± SEM of 3 independent experiments. Statistical significance was determined by one-way analysis of variance (anova) followed by Bonferroni’s test for multiple comparisons using GraphPad Prism 6. p-values comparing untreated and heavy metal treated cells are included. Statistical significance was considered when p-value ≤ 0.05. (c) Immunoblot showing the presence of the fragment (43KD) of active caspase-9 after Cr and Ni exposure to confirm the activation of the intrinsic/mitochondrial pathway. All experiments were performed in triplicates. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31953414), licensed under a CC-BY license. Not internally tested by R&D Systems.
Human/Mouse/Rat Vinculin Antibody

Simple Western: Vinculin Antibody (728526) [Unconjugated] [MAB6896] -

Simple Western: Vinculin Antibody (728526) [Unconjugated] [MAB6896] - Western blot analysis for expression levels of mTOR and its downstream factors in the DRG on PID 21. Phosphorylated expressions of mTOR complex 1 and 2 were decreased in the shmTOR group, and its downstream effectors were downregulated as well. Data are expressed as mean +- SEM, * p < 0.05, ** p < 0.01, **** p < 0.0001. One-way ANOVA with Tukey's multiple comparisons test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/37958901), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse/Rat Vinculin Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human uterus

Simple Western

20 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and PC‑3 human prostate cancer cell line

Western Blot

2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line

Reviewed Applications

Read 10 reviews rated 4.1 using MAB6896 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Vinculin

VCL (vinculin) is a 117 kDa actin binding cytoskeletal phosphoprotein found in adherens-type cell-cell and cell-matrix junctions. The 1066 amino acid (aa) human vinculin contains an N-terminal globular head (aa 2-835) with a talin interaction domain (aa 168-208) and three tandem repeats (aa 259-589), a pro-rich linker region (aa 826-878), and a C-terminal tail that facilitates phospholipid membrane insertion (aa 1003-1134). An 1134 aa form termed metavinculin contains 68 aa inserted after aa 915 and mainly occurs in cardiac, skeletal and smooth muscle. Human, mouse and rat vinculin share 100% aa sequence identity within the region used as an immunogen.

Long Name

Metavinculin

Alternate Names

MVCL, VCL

Entrez Gene IDs

7414 (Human); 22330 (Mouse); 305679 (Rat)

Gene Symbol

VCL

UniProt

P18206

Additional Vinculin Products

Product Documents for Human/Mouse/Rat Vinculin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat Vinculin Antibody

For research use only

Citations for Human/Mouse/Rat Vinculin Antibody

Customer Reviews for Human/Mouse/Rat Vinculin Antibody (10)

4.1 out of 5
10 Customer Ratings
5 Stars
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4 Stars
50%
3 Stars
0%
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1 Stars
10%

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Customer Images

Showing  1 - 5 of 10 reviews Showing All
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  • Human/Mouse/Rat Vinculin Antibody
    Name: Lana Jagannathan
    Application: Western Blot
    Sample Tested: Tumor cell lyastes
    Species: Human
    Verified Customer | Posted 10/14/2025
    We used vinculin as a loading control for western blots of treated cell lines. This vinculin antibody demonstrated a significant amount of unanticipated staining, which we believe is nonspecific since we did not see this issue with a vinculin antibody purchased from another supplier.
    Human/Mouse/Rat Vinculin Antibody MAB6896
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction. To reduce extra bands in your Western blot, we recommend adjusting the primary antibody concentration, % milk or BSA in the solution, and NaCl in the blotting buffer. You can find helpful troubleshooting tips here
  • Human/Mouse/Rat Vinculin Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Human fibroblast
    Species: Human
    Verified Customer | Posted 11/23/2023
    Paraformaldehyde-fixed fibroblast stained with vinculin (red), and counterstained with DAPI (blue)
    Human/Mouse/Rat Vinculin Antibody MAB6896
  • Human/Mouse/Rat Vinculin Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Human osteoblast-like cells (MG63)
    Species: Human
    Verified Customer | Posted 08/27/2021
    Human/Mouse/Rat Vinculin Antibody MAB6896
  • Human/Mouse/Rat Vinculin Antibody
    Name: Krishna Yalla
    Application: Simple Western
    Sample Tested: HCT116 cell line lysate
    Species: Human
    Verified Customer | Posted 08/27/2020
    Vinculin Ab titration on HCT116 cell lysate
    Ab titration on HCT116 cell lysate
    Human/Mouse/Rat Vinculin Antibody MAB6896
  • Human/Mouse/Rat Vinculin Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: IPS2 induced pluripotent stem cells
    Species: Mouse
    Verified Customer | Posted 01/25/2018
    Human/Mouse/Rat Vinculin Antibody MAB6896
  • Human/Mouse/Rat Vinculin Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: HMVEC human microvascular endothelial cells
    Species: Human
    Verified Customer | Posted 12/28/2017
    Human/Mouse/Rat Vinculin Antibody MAB6896
  • Human/Mouse/Rat Vinculin Antibody
    Name: Michele Torino
    Application: Western Blot
    Sample Tested: MCF 10A human breast epithelial cell line and U2OS human osteosarcoma cell line
    Species: Human
    Verified Customer | Posted 12/07/2017
    Human/Mouse/Rat Vinculin Antibody MAB6896
  • Human/Mouse/Rat Vinculin Antibody
    Name: Julie Van
    Application: Western Blot
    Sample Tested: Kidney tissue
    Species: Mouse
    Verified Customer | Posted 08/23/2017
    - 1:3000 dilution of sample size stock - used milk for block and Ab dilution - 10ug of total protein loaded in each well
    Human/Mouse/Rat Vinculin Antibody MAB6896
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Hela whole cell lysate
    Species: Human
    Verified Customer | Posted 10/16/2015
    Hela cells lysate 25 ug protein loaded on SDS gel (6%) used Tris Glycine for electrophoresis. Major bands ~124 kDa
    Human/Mouse/Rat Vinculin Antibody MAB6896
  • Name: Asimina Kerimi
    Application: Simple Western
    Sample Tested: CaCo2 whole cell lysate
    Species: Mouse
    Verified Customer | Posted 09/23/2015
    0.5-1.5 mg/ml of total cell lysate (CaCo2 intestinal cells)
    Human/Mouse/Rat Vinculin Antibody MAB6896

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