Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # 251619
View all STAT5a Primary Antibodies »

Product Specifications

Immunogen

Human STAT5a synthetic peptide
SLDSRLSPPAGLFTSARGSLS
Accession # NP_003143

Specificity

Detects human and mouse STAT5a. Does not cross-react with STAT5b.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Scientific Data Images for Human/Mouse STAT5a Antibody

Detection of Human and Mouse STAT5a antibody by Western Blot.

Detection of Human and Mouse STAT5a by Western Blot.

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and M1 mouse myeloid leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse STAT5a Monoclonal Antibody (Catalog # MAB2174) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for STAT5a at approximately 91 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

STAT5a antibody in K562 Human Cell Line by Immunocytochemistry (ICC).

STAT5a in K562 Human Cell Line.

STAT5a was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Mouse Anti-Human/Mouse STAT5a Monoclonal Antibody (Catalog # MAB2174) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of Mouse STAT5A by Western Blot

Detection of Mouse STAT5A by Western Blot

Effects of inhibition of IGF-IR on IGF-IR, BCR-ABL and downstream target proteins in CML cell lines. (A) PPP induces concentration-dependent decrease in IGF-IR tyrosine kinase activity in K562 and KBM-5 cell lines. In contrast, PPP fails to cause similar effect in BCR-ABL tyrosine kinase activity. The results represent the means ± S.D. of three consistent experiments. *: P < 0.01 and †: P < 0.001 compared with control untreated cells. (B) Western blotting and co-immunoprecipitation studies confirm that PPP decreases the tyrosine phosphorylation of IGF-IR in a concentration-dependent fashion (results shown are representative and were obtained from the KBM-5 cell line). The basal levels of IGF-IR did not change after treatment with PPP. The phosphorylation level of BCR-ABL remains unchanged after treatment with PPP. The decrease in pIGF-IR is associated with down-regulation of pAkt and pSTAT5, two oncogenic proteins in CML. Changes are not seen in Akt and STAT5. Also, PPP induces changes consistent with apoptotic cell death including down-regulation of Bcl-2, Bcl-XL and caspase-3. Moreover, treatment with PPP induces up-regulation of cyclin B1 and down-regulation of cyclin E and pCdc2, whereas the levels of Cdc2 and p16 remain unchanged. Overall, the changes in the cell cycle regulatory proteins are consistent with G2/M-phase cell cycle arrest. beta -Actin shows equal loading of the proteins. (C) IGF-IR siRNA decreases IGF-IR levels in the KBM-5 cell line and this decrease is associated with down-regulation of pAkt and pSTAT5. Basal levels of these two proteins remain unchanged. IGF-IR siRNA did not induce alterations in BCR-ABL or pBCR-ABL protein. beta -Actin confirms equal loading of the proteins. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/19508387), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse STAT5a Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells and K562 human chronic myelogenous leukemia cell line

Western Blot

2 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line and M1 mouse myeloid leukemia cell line

Reviewed Applications

Read 1 review rated 3 using MAB2174 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: STAT5a

STAT family proteins mediate cytokine signaling by acting as signal transducers in the cytoplasm and transcription activators in the nucleus. STAT5a and STAT5b are encoded by separate genes and share 93% amino acid identity.

Long Name

Signal Transducer and Activator of Transcription 5

Alternate Names

MGF, signal transducer and activator of transcription 5A, STAT5

Entrez Gene IDs

6776 (Human); 20850 (Mouse)

Gene Symbol

STAT5A

UniProt

NP_003143

Additional STAT5a Products

Product Documents for Human/Mouse STAT5a Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse STAT5a Antibody

For research use only

Citations for Human/Mouse STAT5a Antibody

Customer Reviews for Human/Mouse STAT5a Antibody (1)

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  • Human/Mouse STAT5a Antibody
    Name: Anonymous
    Application: Simple Western
    Sample Tested: k562 cells
    Species: Human
    Verified Customer | Posted 11/28/2016
    Theoretical pI = 5.98; no peak close to that value Cells used = K562 (human) lysed in Protein Simple bicine/CHAPS buffer (040-764) loaded at 100 ng/µL; Gradient = 5 – 8 nested, ladder# 3 Primary antibody at 1:50 Secondary antibody = Protein Simple biotinylated anti-mouse (041-127) with Protein Simple streptavidin-HRP (041-106) both at 1:100 Predicted m.w. = 90.6 kDa; well defined peak at 95 kDa, good agreement between replicates Cells used = K562 (human) lysed in Protein Simple bicine/CHAPS buffer (040-764) loaded at 500 ng/µL Primary antibody at 1:50
    Human/Mouse STAT5a Antibody MAB2174

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